Effects of single nucleotide polymorphism markers on the carcass and fattening traits in different pig populations
More details
Hide details
Lithuanian University of Health Sciences, Kaunas, 44307, Lithuania
Publication date: 2018-09-21
Corresponding author
R. Bižienė   

Lithuanian University of Health Sciences, Kaunas, 44307, Lithuania
J. Anim. Feed Sci. 2018;27(3):255–262
The objective of this study was to investigate the effect of single nucleotide polymorphisms in 4 different genes: growth hormone (GH), leptin (LEP), growth hormone releasing hormone (GHRH) and myogenic factor 5 (MYF5) on fattening and carcass traits in pigs. The polymorphisms of the porcine genes and their relationships with performance traits were analysed in 143 unrelated pigs, belonging to 5 different breeds (Lithuanian White, old-type Lithuanian White, Large White, Landrace and Yorkshire) and 3 groups of crossbreeds (Large White × Landrace, Yorkshire × Large White, Yorkshire × Pietrain). It was found, that MYF5 polymorphism (Y17154.1: g2200G>C) influenced fattening traits with the highest daily weight gain stated in CC genotype. The most preferable LEP polymorphism (TaqI) (U66254.1: g.1112G>A) was AG genotype with lower age to achieve 100 kg of body weight and average backfat thickness, and higher meatness and weight of ham. It was found that pigs with TT genotype in LEP polymorphism (HinfI) (U66254.1: g.3469T>C) had better carcass properties in comparison to other genotypes. These pigs had also the highest meatness and the lowest average backfat thickness. The preferable GHRH polymorphism (JX435113.1: g.405A>C) genotype was CC with the highest daily weight gain. The most desirable TT genotype of LEP polymorphism (HinfI) (U66254.1: g.3469T>C) was found with the highest frequency in Landrace breed. The highest frequency of the most desirable CC genotype of GHRH gene was found in Yorkshire pig breed. So, from all examined genes LEP and GHRH genes polymorphisms seem to be the most preferable biomarkers of pig selection process.
Blicharski T., Kurył J., Pierzchała M., 2004. Relationship between polymorphism at loci colipase and leptin and most important fattening and slaughter traits in pigs with special reference to intramuscular fat – a review (in Polish). Pr. Mater. Zootech. Zesz. Spec. 15, 41–46.
Caraballo C., Muñoz M., Rodríguez C., Silió L., García-Casco J.M., 2018. Racial verification of Iberian ham and shoulders commercialized in Spanish supermarkets. Arch. Zootec. 68 (Supp. 1), 5–8, ref. 7.
Chikuni K., Tanabe R., Muroya S., Fukumoto Y., Ozawa S., 1997. A simple method for genotyping the bovine growth hormone gene. Anim. Genet. 28, 230–232, https://doi.org/10.1111/j.1365....
Curi R.A., de Oliveira H.N., Silveira A.C., Lopes C.R., 2005. Effects of polymorphic microsatellites in the regulatory region of IGF1 and GHR on growth and carcass traits in beef cattle. Anim. Genet. 36, 58–62, https://doi.org/10.1111/j.1365....
De Oliveira Peixoto J., Facioni Guimarães S.E, Sávio Lopes P., Menck Soares M.A., Vieira Pires A., Gualberto Barbosa M.V., De Almeida Torres R., De Almeida e Silva M., 2006. Associations of leptin gene polymorphisms with production traits in pigs. J. Anim. Breed. Genet. 123, 378–383, https://doi.org/10.1111/j.1439....
Franco M.M., Antunes R.C., Silva H.D., Goulart L.H., 2005. Association of PIT1, GH and GHRH polymorphisms with performance and carcass traits in Landrace pigs. J. Appl. Genet. 46, 195–200.
Georgescu S.E., Manea M.A., Dinescu S., Costache M., 2014. Comparative study of leptin and leptin receptor gene expression in different swine breeds. Genet. Mol. Res. 13, 7140–7148, https://doi.org/10.4238/2014.F....
Grochowska R., Zwierzchowski L., Snochowski M., Reklewski Z., 1999. Stimulated growth hormone (GH) release in Friesian cattle with respect to GH genotypes. Reprod. Nutr. Dev. 39, 171–180, https://doi.org/10.1051/rnd:19....
Jiang Z.-H., Gibson J.P., 1999. Genetic polymorphisms in the leptin gene and their association with fatness in four pig breeds. Mamm. Genome 10, 191–193, https://doi.org/10.1007/s00335....
Kennes Y.M., Murphy B.D., Pothier F., Palin M.-F., 2001. Characterization of swine leptin (LEP) polymorphisms and their association with production traits. Anim. Genet. 32, 215–218, https://doi.org/10.1046/j.1365....
Kim J., Chung H., Kim S., Park H., Kewon D.-H., 2009. Discovery of single nucleotide polymorphisms in FABP3 and leptin gene in pig. Arch. Anim. Breed. 52, 106–107, https://doi.org/10.5194/aab-52....
Knorr C., Moser G., Müller E., Geldermann H., 1997. Associations of GH gene variants with performance traits in F2 generations of European wild boar, Piétrain and Meishan pigs. Anim. Genet. 28, 124–128, https://doi.org/10.1111/j.1365....
Křenková L., Kuciel J., Urban T., 1999. Association of the RYR1, GH, LEP and TF genes with carcass and meat quality traits in pigs. Czech J. Anim. Sci. 44, 481–486.
Kulig H., Grzesiak W., Szatkowska I., 2001. Effect of leptin gene polymorphism on growth and carcass traits in pigs. Arch. Anim. Breed. 2001. 44, 291–296, https://doi.org/10.5194/aab-44....
Lee T., Shin D.-H., Cho S., Kang H.S., Kim S.H., Lee H.-K., Kim H., Seo K.-S., 2014. Genome-wide association study of integrated meat quality-related traits of Duroc pig breed. AsianAustralas. J. Anim. Sci. 27, 303–309, https://doi.org/10.5713/ajas.2....
Li J., Ran X.-Q., Wang J.F., 2006. Identification and function of the growth hormone gene in Rongjiang pig of China. Acta Physiol. Sinica 58, 217–224.
Pierzchała M., Blicharski T., Kurył J., 2003. Growth rate and carcass quality in pigs as related to genotype at loci POU1F1/RsaI (Pit1/RsaI) and GHRH/AluI. Anim. Sci. Pap. Rep. 21, 159–166.
Pierzchała M., Blicharski T., Kurył J., 2004. Growth rate and carcass quality in relation to GH/MspI and GH/HaeII PCR-RFLP polymorphism in pigs. Anim. Sci. Pap. Rep. 22, 57–64.
Rejduch B., 2008. Genes associated with production and health in farm animals. J. Cent. European Agric. 9, 829–836.
Song C.Y., Gao B., Jing R.B., Tao Y., Mao J.D., 2003. Study on pig growth hormone gene polymorphisms in western meat-type breeds and Chinese local breeds. J. Zhejiang Univ. Sci. 4, 734–739, https://doi.org/10.1631/jzus.2....
Stępień-Poleszak D., Pietruszka A., Kawęcka M., 2009. Effect of leptin gene polymorphism on fattening and slaughter value of Line 990 gilts. Acta Vet. Brno 78, 267–272, https://doi.org/10.2754/avb200....
te Pas M.FW., Soumillion A., Harders F.L., Verburg F., van den Bosch T.J., Galesloot P., Meuwissen T.H.E., 1999. Influences of myogenin genotypes on birth weight, growth rate, carcass weight, backfat thickness, and lean weight of pigs. J. Anim. Sci. 77, 2352–2356, https://doi.org/10.2527/1999.7....
Urban T., Mikolášová R., 2006. Genetic variability in the leptin receptor and heart fatty acid binding protein genes in pigs. Acta Fytotechn. Zootechn. 9, 29–31.
Urbański P., Pierzchała M., Kamyczek M., Różycki M., Kurył J., 2006. Relations between the polymorphism in the coding and 5’-flanking regions of the porcine MYOD1 and MYF5 genes and productive traits in pigs. J. Anim. Feed Sci. 15, 225–235, https://doi.org/10.22358/jafs/....
Wang W., Huang L., Gao J., Ding J., Chen K., Ren J., Luo M., 2003. Polymorphism of growth hormone gene in 12 pig breeds and its relationship with pig growth and carcass traits. AsianAustralas. J. Anim. Sci. 16, 161–164, https://doi.org/10.5713/ajas.2....
Wang W., Xue W., Zhou X., Zhang L., Wu J., Qu L., Jin B., Zhang X., Ma F., Xu X., 2012. Effects of candidate genes’ polymorphisms on meat quality traits in pigs. Acta Agric. Scand. A Anim. Sci. 62, 120–126, https://doi.org/10.1080/090647....
Zhu C., Gi G., Tao Z., Song C., Zhu W., Song W, Li H., 2014. Development of skeletal muscle and expression of myogenic regulatory factors during embryonic development in Jinding ducks (Anas platyrhynchos domestica). Poult. Sci. 93, 1211–1216, https://doi.org/10.3382/ps.201....
Leptin Gene Polymorphism in Goats Fed with Diet at Different Energy Level: Effects on Feed Intake, Milk Traits, Milk Fatty Acids Composition, and Metabolic State
Marcella Avondo, Trana Di, Bernardo Valenti, Andrea Criscione, Salvatore Bordonaro, Angelis De, Daniela Giorgio, Gregorio Di
Asociación de polimorfismos del gen Leptina con calidad seminal en toros raza Carora
Belkys Vásquez-Marín, Saúl Salazar-Sequea, La De, Omar Verde, Alexis Marques-Urdaneta, Lourdes Vilanova-Fernández
Revista Científica de la Facultad de Ciencias Veterinarias
Low- and high-protein diets supplemented up to the fourth limiting amino acid for genetic lines of grower-finisher pigs
Fábio Souza, Jansller Genova, Cristine Gregory, Oliveira de, Leandro Castilha, Cinthia Eyng, Ricardo Nunes, Silvana Carvalho, Cláudio Ribeiro, Paulo Carvalho
Livestock Science
Associations of Polymorphisms in Leptin and Leptin Receptor Genes with Meat Quality in Pigs of the Ukrainian Large White Breed
V. Balatsky, Y. Oliinychenko, A. Saienko, T. Buslyk, I. Bankovska, M. Peka, O. Doran
Cytology and Genetics