ORIGINAL PAPER
 
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ABSTRACT
The aim of the present study was to investigate the effects of dietary inclusion of Tongmai granule residue (TGR) on growth performance, nutrient digestibility, blood biochemical parameters and rumen fermentation in sheep. Twenty-four healthy Doper × Small Tail Han hybrid female lambs (6–7 months of age; 29.64 ± 1.80 kg initial body weight) were randomly allocated into 3 groups and fed diets to which 0, 10 and 15% TGR were added for 75 days. It was shown that TGR dietary inclusion at the level of 15% decreased growth performance, neutral detergent fibre apparent digestibility, rumen contents of acetic, propionic, butyric acids and total volatile fatty acids (P < 0.05), but increased crude fat and organic matter (OM) apparent digestibilities, serum high-density lipoprotein cholesterol, immunoglobulin (Ig)A, IgG, IgM, rumen pH, NH3-N concentration and acetic:propionic acid ratio in lambs (P < 0.05). In the 10% TGR group, OM apparent digestibility and IgA also increased (P < 0.05), however, no negative effects on average daily weight gain, rumen pH, NH3-N, volatile fatty acids concentration and blood biochemical parameters were observed. So, it is suggested that TGR could be used as a feed source in sheep production, and 10% TGR inclusion is the most suitable dose.
ACKNOWLEDGEMENTS
We thank Research Square for preprinting this manuscript online according to the following link: https://www.researchsquare.com article/rs-60214/ v1 (Research Square doi:10.21203/rs.3.rs-60214/ v1), so that we could get more comments to improve it.
CONFLICT OF INTEREST
The Authors declare that there is no conflict of interest.
FUNDING
The authors thank the National Key Research and Development Program of China (Grant No. 2016YFD0501209-03) and the National Natural Science Foundation of China (Grant No. 3177260).
 
REFERENCES (30)
1.
Alagawany M., Abd El-Hack M.E., 2015. The effect of rosemary herb as a dietary supplement on performance, egg quality, serum biochemical parameters, and oxidative status in laying hens. J. Anim. Feed Sci. 24, 341−347, https://doi.org/10.22358/jafs/....
 
2.
Chung M.J., Sung N.J., Park C.S., Kweon D.K., Mantovani A., Moon T.W., Lee S.J., Park K.H., 2008. Antioxidative and hypocholesterolemic activities of water-soluble puerarin glycosides in HepG2 cells and in C57 BL/6J mice. Eur. J. Pharmacol. 578, 159−170, https://doi.org/10.1016/j.ejph....
 
3.
Gao H.J., Liu P.F., Li P.W., Huang Z.Y., Yu, F.B., Lei T., Chen Y., Cheng Y., Mu Q.C., Huang, H.Y., 2015. Ligustrazine monomer against cerebral ischemia-reperfusion injury. Neural Regen. Res. 10, 832−840, https://doi.org/10.4103/1673-5....
 
4.
Gong J., Yin F., Hou Y., Yin Y., 2014. Review: Chinese herbs as alternatives to antibiotics in feed for swine and poultry production: potential and challenges in application. Can. J. Anim. Sci. 94, 223−241, https://doi.org/10.4141/cjas20....
 
5.
Guo C., Xu L., He Q., Liang T., Duan X., Li R., 2013. Anti-fibrotic effects of puerarin on CCl4‐induced hepatic fibrosis in rats possibly through the regulation of PPAR‐γ expression and inhibition of PI3K/Akt pathway. Food Chem. Toxicol. 56, 436−442, https://doi.org/10.1016/j.fct.....
 
6.
Guo F.C., Kwakkel R.P., Soede J., Williams B.A., Verstegen M.W.A., 2004. Effect of a Chinese herb medicine formulation, as an alternative for antibiotics, on performance of broilers. Br. Poult. Sci. 45, 793−797, https://doi.org/10.1080/000716....
 
7.
Guo Z.M., Li P., Xia S.L., Wang C., Wang W.J., Liu S.H., Wang J.Q., Liu Y., 2010. Effect of Salvia miltiorrhiza residue on performance of mutton sheep. Tianjin Agric. Sci. 16(5), 45−46.
 
8.
Hao Z.M., Wang J., Zhu L.J., Wang J.B., 2011. Effects of acetate/propionate on in vitro fermentation and microbial protein production. Guangdong Feed 20(8), 20−24.
 
9.
He J., 2008. Effects of Salvia miltiorrhiza on antibody production in mice. Chin. Med. Guide 19, 157−158.
 
10.
Huang K., 2009. Extraction and separation of effective components from radix puerariae. PhD Thesis. Wuhan Institute of Technology, Wuhan (China).
 
11.
Lin H.Z., Li Z.J., Chen Y.Q., Zheng W.H., Yang K., 2006. Effect of dietary traditional Chinese medicines on apparent digestibility coefficients of nutrients for white shrimp Litopenaeus vannamei, Boone. Aquaculture 253, 495−501, https://doi.org/10.1016/j.aqua....
 
12.
Li R., Xu L., Liang T., Li Y., Zhang S., Duan X., 2013. Puerarin mediates hepatoprotection against CCl4‐induced hepatic fibrosis rats through attenuation of inflammation response and amelioration of metabolic function. Food Chem. Toxicol. 52, 69−75, https://doi.org/10.1016/j.fct.....
 
13.
Li X.Y., 2015. Effects of ginger root, star anise and dan-shen root on growth, carcass performance and antioxidant status of Laiwu Black pigs. Master Thesis. Shangdong Agricultural University, Tai an (China).
 
14.
Li Y., Wang Y.Z., Ding X., Zhang Y.G., Xue S.C., Lin C., Xu W.B., Dou X.J., Zhang L.Y., 2016. Effects of Acremonium terricola culture on growth performance, antioxidant status and immune functions in weaned calves. Livest. Sci. 193, 66−70, https://doi.org/10.1016/j.livs....
 
15.
Li Y., Sun Y.K., Li X., Zhang G.N., Xin H.S., Xu H.J., Zhang L.Y., Li X.X., Zhang Y.G., 2018. Effects of Acremonium terricola culture on performance, milk composition, rumen fermentation and immune functions in dairy cows. Anim. Feed Sci. Technol. 240, 40−51, https://doi.org/10.1016/j.anif....
 
16.
Liu B.Y., Du Y.H., Cong L.X., Jia X., Yang G., 2016. Danshen (Salvia miltiorrhiza) compounds improve the biochemical indices of the patients with coronary heart disease. Evid.-Based Complementary Altern. Med. 2016, 9781715, https://doi.org/10.1155/2016/9....
 
17.
Meng F., Yang S., Wang X., Chen T., Wang X., Tang X., Zhang R., Shen L., 2017. Reclamation of Chinese herb residues using probiotics and evaluation of their beneficial effect on pathogen infection. J. Infect. Public Heal. 10, 749−754, https://doi.org/10.1016/j.jiph....
 
18.
Noh B.K., Lee J.K., Jun H.J., Lee J.H., Jia Y., Hoang M.H., Kim J.W., Park K.H., Lee S.J., 2011. Restoration of autophagy by puerarin in ethanol‐treated hepatocytes via the activation of AMP‐activated protein kinase. Biochem. Biophys. Res. Commun. 414, 361−366, https://doi.org/10.1016/j.bbrc....
 
19.
NRC (National Research Council), 2011. Guide for the Care and Use of Laboratory Animals. Eighth Edition. The National Academies Press. Washington, DC (USA), https://doi.org/10.17226/12910.
 
20.
Owens F.N., Bergen W.G., 1983. Nitrogen metabolism of ruminant animals: historical perspective, current understanding and future implications. J. Anim. Sci. 57, 498−518.
 
21.
Shang H.L., Song X.Z., Liu B., Xu Y., Zhang X.Y., Yang S.T., 2018. Effects of puerarin on performance and blood biochemical indices of beef cattle under high temperature in summer. J. Jiangxi Agric. Uni. 40, 803−809.
 
22.
Shi L., Yu H.S., Jin F.X., 2010. Study on Tanshinone extracted from Salvia miltiorrhiza Bge. Dregs. Agric. Sci. Technol. 11, 103−106, https://doi.org/10.16175/j.cnk....
 
23.
Wang C., Liu Q., Guo G., Huo W.J., Ma L., Zhang Y.L., Pei C.X., Zhang S.L., Wang H., 2016. Effects of dietary supplementation of rumen-protected folic acid on rumen fermentation, degradability and excretion of urinary purine derivatives in growing steers. Arch. Anim. Nutr. 70, 441−454, https://doi.org/10.1080/174503....
 
24.
Wang F.R., Zhang Y., Yang X.B., Liu C.X., Yang X.W., Xu W., Liu J.X., 2017. Rapid Determination of 30 polyphenols in Tongmai formula, a combination of Puerariae Lobatae Radix, Salviae Miltiorrhizae Radix et Rhizoma, and Chuanxiong Rhizoma, via liquid chromatography-tandem mass spectrometry. Molecules 22, 545, https://doi.org/10.3390/molecu....
 
25.
Wu S., Zhou Q.L., Yang X.W., 2017. UFLC-DAD-ESI-IT-TOFMSn Analysis on biotransformation of Tongmai formula incubated with human intestinal bacteria. Chin. Herb. Med. 9, 258−266, https://doi.org/10.1016/S1674-....
 
26.
Xia S.L., Li P., Guo Z.M., Wang C., Wang W.J., Liu S.H., Wang J.Q., Liu Y., 2009. Effects of Salvia miltiorrhiza residue and Yangxue residue on performance of beef cattle. Tianjin Agric. Sci. 15(5), 17−18.
 
27.
Xie M.X., 2018. Effect of Sophora alopecuroides L. on rumen fermentation parameters, ruminal microbial population and blood physiological-biochemical indexes of mongolian Lamb. Master Thesis. Inner Mongolia Agricultural University, Hohhot (China).
 
28.
Xu W.Q., 2005. Study on Extraction, Purification and Stability Ligustrazine. Master Thesis. Beijing University of Chemical Technology, Beijing (China).
 
29.
Zhang Q., 2011. The Assessment of Suitability in Cow Feeding with Wild Kudzu Root. Master Thesis. Zhengzhou University Zhengzhou (China).
 
30.
Zhao Y., Liu Y., Chen K., 2016. Mechanisms and clinical application of tetramethylpyrazine (an interesting natural compound isolated from Ligusticum Wallichii): current status and perspective. Oxid. Med. Cell. Longev. 2016, 2124638, https://doi.org/10.1155/2016/2....
 
ISSN:1230-1388