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Effect of different storage temperatures on the metabolic activity of spermatozoa following liquid storage of boar semen

L. Fraser 1,  
J. Strzeżek 1  
Warmia and Mazury University in Olsztyn, Department of Animal Biochemistry and Biotechnology, Oczapowskiego 5, 10-718 Olsztyn-Kortowo, Poland
J. Anim. Feed Sci. 2009;18(4):638–649
Publication date: 2009-11-06
This study investigated the effects of different temperatures (5º and 16ºC) on the metabolic activity of boar spermatozoa preserved in a standard semen extender, Kortowo 3 (K3), supplemented with or without egg yolk lipoprotein fractions (LPF) isolated from hen or ostrich egg yolk. Besides motility and plasma membrane integrity (PMI) assessments of spermatozoa, analysis of the metabolic activity of spermatozoa included mitochondrial energy status, oxygen uptake, ATP content and L-lactate production. Total motility and PMI of spermatozoa decreased over time. The metabolic activity of spermatozoa was significantly higher in the extenders containing LPF than in the K3 extender. Marked changes in the sperm metabolic activity observed during storage of semen samples at 5ºC indicated that glycolysis (fructolysis) was the major metabolic pathway. It seemed likely that mitochondrial respiration was the predominant metabolic activity exhibited by spermatozoa during storage at 16ºC. The findings of this study indicate that different storage temperatures can modulate the metabolic pathways of boar spermatozoa during liquid semen storage.
J. Strzeżek   
Warmia and Mazury University in Olsztyn, Department of Animal Biochemistry and Biotechnology, Oczapowskiego 5, 10-718 Olsztyn-Kortowo, Poland
1. Boar variability affects sperm metabolism activity in liquid stored semen at 5°C
A. Dziekońska, J. Strzeżek
Polish Journal of Veterinary Sciences
2. The benefits of cooling boar semen in long-term extenders prior to cryopreservation on sperm quality characteristics
K Wasilewska, Ł Zasiadczyk, L Fraser, M Mogielnicka-Brzozowska, W Kordan
Reproduction in Domestic Animals
3. Boar variability in sperm cryo-tolerance after cooling of semen in different long-term extenders at various temperatures
K. Wasilewska, L. Fraser
Animal Reproduction Science
4. Missense mutation in glutathione-S-transferase M1 gene is associated with sperm motility and ATP content in frozen-thawed semen of Holstein-Friesian bulls
D.M. Hering, M. Lecewicz, W. Kordan, A. Majewska, S. Kaminski
Animal Reproduction Science
5. Metabolic activity of boar semen stored in different extenders supplemented with ostrich egg yolk lipoproteins
Anna Dziekońska, Marek Kinder, Leyland Fraser, Jerzy Strzeżek, Władysław Kordan
Journal of Veterinary Research
6. Association Between ETFA Genotype and Activity of Superoxide Dismutase, Catalase and Glutathione Peroxidase in Cryopreserved Sperm of Holstein-Friesian Bulls
DM Hering, M Lecewicz, W Kordan, S Kamiński
Reproduction in Domestic Animals
7. Effect of platelet activating factor (PAF) supplementation in semen extender on viability and ATP content of cryopreserved canine spermatozoa
W. Kordan, M. Lecewicz, R. Strzeżek, A. Dziekońska, L. Fraser
Polish Journal of Veterinary Sciences
8. Individual and seasonal variations in the quality of fractionated boar ejaculates
L. Zasiadczyk, L. Fraser, W. Kordan, K. Wasilewska
9. Genome-wide association study for sperm membrane integrity in frozen-thawed semen of Holstein-Friesian bulls
Stanisław Kamiński, Dorota M. Hering, Kamil Oleński, Marek Lecewicz, Władysław Kordan
Animal Reproduction Science
10. Effects of cryopreservation on sperm viability, synthesis of reactive oxygen species, and DNA damage of bovine sperm
H. Gürler, E. Malama, M. Heppelmann, O. Calisici, C. Leiding, J.P. Kastelic, H. Bollwein
11. Effect of ostrich egg lipoproteins and hen egg yolk on the quality of dog sperm during liquid storage at 5 °C
Anna Dziekońska, Dorota Behrendt, Rafał Strzeżek, Władysław Kordan
Annals of Animal Science
12. Mitochondria-targeted antioxidant MitoTEMPO improves the post-thaw sperm quality
Xilan Lu, Yingchun Zhang, Hongwei Bai, Jingjing Liu, Juntao Li, Bin Wu
13. Identification of genome regions determining semen quality in Holstein-Friesian bulls using information theory
Alicja Borowska, Tomasz Szwaczkowski, Stanisław Kamiński, Dorota M. Hering, Władysław Kordan, Marek Lecewicz
Animal Reproduction Science
14. Effects of the platelet-activating factor (PAF) on selected quality parameters of cryopreserved bull semen (AI) with reduced sperm motility
M. Lecewicz, W. Kordan, A. Majewska, S. Kamiński, A. Dziekońska, K. Mietelska
Polish Journal of Veterinary Sciences
15. Effects of storage in different semen extenders on the pre-freezing and post-thawing quality of boar spermatozoa
A. Dziekońska, Ł. Zasiadczyk, M. Lecewicz, R. Strzeżek, M. Koziorowska-Gilun, L. Fraser, M. Mogielnicka-Brzozowska, W. Kordan
Polish Journal of Veterinary Sciences
16. Seasonal-dependent variations in metabolic status of spermatozoa and antioxidant enzyme activity in the reproductive tract fluids of wild boar/domestic pig hybrids
A. Dziekońska, L. Fraser, M. Koziorowska-Gilun, J. Strzeżek, M. Koziorowski, W. Kordan
Polish Journal of Veterinary Sciences
17. Semen quality assessments and their significance in reproductive technology
W. Kordan, L. Fraser, P. Wysocki, R. Strzeżek, M. Lecewicz, M. Mogielnicka-Brzozowska, A. Dziekońska, D. Soliwoda, M. Koziorowska-Gilun
Polish Journal of Veterinary Sciences
18. Effect of commercial long-term extenders on metabolic activity and membrane integrity of boar spermatozoa stored at 17°C
A. Dziekońska, L. Fraser, A. Majewska, M. Lecewicz, Ł. Zasiadczyk, W. Kordan
Polish Journal of Veterinary Sciences
19. Interactions of egg yolk lipoprotein fraction with boar spermatozoa assessed with a fluorescent membrane probe.
Leyland Fraser, Łukasz Zasiadczyk, Jerzy Strzezek
Folia Histochemica et Cytobiologica
20. Effect of extender supplementation with low-molecular-weight antioxidants on selected quality parameters of cryopreserved canine spermatozoa
Marek Lecewicz, Rafał Strzeżek, Władysław Kordan, Anna Majewska
Journal of Veterinary Research
21. Photostimulation and thermotaxis of sperm: overview and practical implications in porcine reproduction
Joan Rodríguez-Gil
22. Use of the ACP® and BTS extenders for cooling at 15°C white-lipped peccary (Tayassu pecari) semen
Celso Barros, William Machado, Renan Vieira, Ivan Allaman, Sérgio Nogueira-Filho, Rodrigo Bittencourt, Paola Snoeck
Pesquisa Veterinária Brasileira
23. Effect of seminal plasma zinc–binding proteins on motility and membrane integrity of canine spermatozoa stored at 5°C
Marzena Mogielnicka-Brzozowska, Anna Dziekońska, Rafał Strzeżek, Michał Załęcki, Anna Majewska, Karolina Tołścik, Władysław Kordan
Bulletin of the Veterinary Institute in Pulawy
24. Transcriptome analysis of boar spermatozoa with different freezability using RNA-Seq
L. Fraser, P. Brym, C.S. Pareek, M. Mogielnicka-Brzozowska, Ł. Paukszto, J.P. Jastrzębski, K. Wasilewska-Sakowska, A. Mańkowska, P. Sobiech, K. Żukowski
25. Effect of boar seminal dose type (cervical compared with post-cervical insemination) on cooling curve, sperm quality and storage time
C. Luongo, G Garrappa, PJ. Llamas-López, E. Rodríguez-Tobón, R. López-Úbeda, S. Abril-Sánchez, FA. García-Vázquez
Animal Reproduction Science
26. Gene Polymorphisms in Boar Spermatozoa and Their Associations with Post-Thaw Semen Quality
Anna Mańkowska, Paweł Brym, Łukasz Paukszto, Jan Jastrzębski, Leyland Fraser
International Journal of Molecular Sciences
27. The role of environmental optimization for storing bulls’ sperm cells
Alicja Kowalczyk, Marian Kuczaj, Ewa Czerniawska-Piątkowska
Systems Biology in Reproductive Medicine
28. Regulatory Potential of Long Non-Coding RNAs (lncRNAs) in Boar Spermatozoa with Good and Poor Freezability
Leyland Fraser, Łukasz Paukszto, Anna Mańkowska, Paweł Brym, Przemysław Gilun, Jan Jastrzębski, Chandra Pareek, Dibyendu Kumar, Mariusz Pierzchała