Ejaculates, collected over a 35-month period from 3 Polish Landrace boars from 8 months of
age, were diluted (30 x 106sperm/cm3) with a standard extender, Kortowo 3 (K3) supplemented with
lipoprotein fractions extracted from hen (K3-LPFh) or ostrich egg yolk (K3-LPFo). Thereafter, the
diluted sperm samples were stored for 4 days (D0 to D4) at 5° and 16°C, respectively. Irrespective
of the extender composition and storage temperatures, sperm motility was gradually reduced during
prolonged storage. The decline in sperm motility was more accentuated in K3 extender compared
with LPF-based extenders. Seasonal influence was more apparent in K3-LPFo extender during
storage at 5°C.
The results of this study demonstrate that the inclusion of lipoprotein fractions in semen extender
enhanced cold shock resistance of boar spermatozoa, particularly during storage at 5°C.
CITATIONS(1):
1.
Effect of long-term storage in Safe Cell+ extender on boar sperm DNA integrity and other key sperm parameters Wiesław Bielas, Wojciech Niżański, Agnieszka Partyka, Anna Rząsa, Ryszard Mordak Acta Veterinaria Scandinavica