Effect of extender composition and storage temperatures on motility of preserved boar semen
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Warmia and Mazury University in Olsztyn, Faculty of Animal Bioengineering, Department of Animal Biochemistry, Oczapowskiego 5, 10-718 Olsztyn, Poland
Publication date: 2002-10-11
J. Anim. Feed Sci. 2002;11(4):661–669
Ejaculates, collected over a 35-month period from 3 Polish Landrace boars from 8 months of age, were diluted (30 x 106sperm/cm3) with a standard extender, Kortowo 3 (K3) supplemented with lipoprotein fractions extracted from hen (K3-LPFh) or ostrich egg yolk (K3-LPFo). Thereafter, the diluted sperm samples were stored for 4 days (D0 to D4) at 5° and 16°C, respectively. Irrespective of the extender composition and storage temperatures, sperm motility was gradually reduced during prolonged storage. The decline in sperm motility was more accentuated in K3 extender compared with LPF-based extenders. Seasonal influence was more apparent in K3-LPFo extender during storage at 5°C. The results of this study demonstrate that the inclusion of lipoprotein fractions in semen extender enhanced cold shock resistance of boar spermatozoa, particularly during storage at 5°C.
Effect of long-term storage in Safe Cell+ extender on boar sperm DNA integrity and other key sperm parameters
Wiesław Bielas, Wojciech Niżański, Agnieszka Partyka, Anna Rząsa, Ryszard Mordak
Acta Veterinaria Scandinavica