Fatty acid (FA) composition of adipose tissue and breast muscle was determined in broiler chickens fed from 8 day of life till slaughter on wheat-soyabean meal diets supplemented with different fats. The following fats and fat sources were used: beef tallow, lard, soya oil, rape seed oil or mixtures of tallow and soya oil, linseed seed and lard or rape seed and lard. The dietary fatty acids (FA) profile affected FA profile of lipids and the proportion of n-6/n-3 PUFA in broilers. The supplementation of diet with beef tallow caused the highest surplus n-6 over n-3 PUFA in adipose and breast muscle (11.9 and 10.3, respectively). The proportion of n-6/n-3 PUFA in adipose tissue and breast muscle of birds fed on diet supplemented with the mixture of full-fat linseed and lard (1.2 and 1.6, respectively) was the closest to the proportion of these fatty acids recommended for humans.