ORIGINAL PAPER
Is royal jelly a sustainable alternative lipid source
in aquaculture? Influence of dietary royal jelly levels
on fatty acid composition in zebrafish
1
Akdeniz University, Faculty of Agriculture, Department of Agricultural Biotechnology, Division of Animal Biotechnology,
07058 Antalya, Türkiye
2
Akdeniz University, Kumluca Vocational School, Department of Plant and Animal Production, 07350 Antalya, Türkiye
3
Bingöl University, Faculty of Health Sciences, Department of Occupational Health and Safety, 12000 Bingöl, Türkiye
4
Warsaw University of Life Science, Institute of Animal Sciences, Department of Ichthyology and Biotechnology in Aquaculture,
Ciszewskiego 8, 02-786 Warsaw, Poland
Publication date: 2023-05-15
J. Anim. Feed Sci. 2023;32(4):459-467
KEYWORDS
TOPICS
ABSTRACT
Royal jelly (RJ) is a bee-derived product widely used as
a dietary supplement due to its high potential health benefits. The present study
was designed to investigate the effect of feeding RJ on zebrafish fatty acid
composition. Zebrafish were fed five distinct diets (D1, D2, D3, D4, and D5)
for 56 days supplemented with 0.0, 0.1, 0.4, 1.6, and 6.4% RJ, respectively.
Gas chromatography-mass spectrometry and fatty acid methyl ester (FAME)
analyses were used to determine FA content in the whole body of zebrafish.
The results showed that a feeding regimen that included incrementing RJ doses
resulted in statistically significant increases in 16:0, 18:2n-6, and the ratio of
eicosapentaenoic acid (EPA) to docosahexaenoic acid (DHA) (P < 0.05). In
particular, the content of EPA/DHA increased up to 1.3-fold compared to control
group D1. Conversely, increasing RJ levels led to significant decreases in DHA,
HUFA, and n-3/n-6 PUFA levels in relation to group D1 (P < 0.05). In conclusion,
considering the EPA/DHA ratio among the analysed fatty acids, the diet with a
1.6% RJ addition was the optimal choice. Therefore, RJ can be recommended
as a sustainable alternative lipid source in aquaculture.
ACKNOWLEDGEMENTS
This study was supported by TUBİTAK (The
Scientific and Technological Research Institution
of Turkey) under the code TUBİTAK-COST (European
Cooperation in Science and Technology)
[114O755].
CONFLICT OF INTEREST
The Authors declare that there is no conflict of
interest.
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