REVIEW PAPER
Development of pig cloning studies: past, present and future
M. Samiec 1  
 
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National Research Institute of Animal Production, Department of Animal Reproduction, 32-083 Balice, Poland
CORRESPONDING AUTHOR
M. Samiec   

National Research Institute of Animal Production, Department of Animal Reproduction, 32-083 Balice, Poland
Publication date: 2004-04-09
 
J. Anim. Feed Sci. 2004;13(2):211–238
 
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ABSTRACT
The birth of the frst nuclear transfer-derived cloned piglet resulted from the transfer of a nucleus from a 4-cell stage embryo to an enucleated oocyte. Although biotechnological procedures in swine have recently undergone tremendous progress, the efficiency of pig somatic cloning is still lower than in other species of farm animals and, when expressed as a proportion of reconstructed oocytes, it does not exceed an average of 5 to 10% blastocysts and 1.5% born piglets. The basic prerequisite for practical application of this method is to improve efficiency. This requires further detailed studies. Development of pig somatic cell cloning was inspired not only by the necessity for quick improvement of the efficiency of the nuclear transfer technique in this species, but above all by its possible practical application for multiplication of transgenic piglets, for use in transplantation medicine and immunology, also pharmacy and animal breeding. A deficit of organs for human allotransplantation became a stimulus to the search for new, alternative sources of grafts. Therefore, a particularly attractive aspect of pig somatic cloning is the possibility of generating large numbers of transgenic pig organs for xenotransplantation. Compatibility with the human ones in size, anatomy, and physiology makes this an attractive proposition.
 
CITATIONS (6):
1.
Trichostatin A-Mediated Epigenetic Transformation of Adult Bone Marrow-Derived Mesenchymal Stem Cells Biases theIn VitroDevelopmental Capability, Quality, and Pluripotency Extent of Porcine Cloned Embryos
Marcin Samiec, Jolanta Opiela, Daniel Lipiński, Joanna Romanek
BioMed Research International
 
2.
Effect of histone deacetylase inhibitor romidepsin on the in vitro growth of foetal fibroblast cells and early development of porcine-cloned embryos
Zhangfan Wang, Chengxue Liu, Renyun Hong, Jie Yang, Hui Li, Yunsheng Li, Hongguo Cao, Yunhai Zhang, Zubing Cao
Italian Journal of Animal Science
 
3.
Pseudophysiological transcomplementary activation of reconstructed oocytes as a highly efficient method used for producing nuclear-transferred pig embryos originating from transgenic foetal fibroblast cells
M. Samiec, M. Skrzyszowska, D. Lipiński
Polish Journal of Veterinary Sciences
 
4.
The possibilities of practical application of transgenic mammalian species generated by somatic cell cloning in pharmacology, veterinary medicine and xenotransplantology
M. Samiec, M. Skrzyszowska
Polish Journal of Veterinary Sciences
 
5.
CRISPR/Cas9-Mediated Biallelic Knockout of IRX3 Reduces the Production and Survival of Somatic Cell-Cloned Bama Minipigs
Xiangxing Zhu, Yanyan Wei, Qunmei Zhan, Aifen Yan, Juan Feng, Lian Liu, Dongsheng Tang
Animals
 
6.
Technical, biological and molecular aspects of somatic cell nuclear transfer – a review
Patrycja Mrowiec, Monika Bugno-Poniewierska
Annals of Animal Science
 
ISSN:1230-1388