0.857
IF5
0.900
IF
Q3
JCR
0.92
CiteScore
0.405
SJR
Q2
SJR
20
MNiSW
165.24
ICV
ORIGINAL PAPER
 
CC-BY 4.0
 
 

Determination of endogenous nitrogen associated with bacteria in ileal digesta of pigs receiving cereal-based diets with or without fish meal and various fibre supplements by using a simple 15N-dilution technique

J. Bartelt 1,  
W. Drochner 2,  
K.-P. Götz 3,  
J. Szakacz 4,  
 
1
Department of Animal Nutrition, Free University of Berlin, Brümmerstr. 34, 14195 Berlin, Germany
2
Department of Animal Nutrition, Hohenheim University, Emil-Wolff-Str. 10, 70599 Stuttgart, Germany
3
Department of Crop Science, Humboldt University Berlin, Albrecht-Thaer-Weg 5, 14195 Berlin, Germany
4
Research Institute of Animal Production, Hlohovská 2, 94992 Nitra, Slovakia
J. Anim. Feed Sci. 1999;8(3):425–440
Publish date: 1999-07-05
KEYWORDS
ABSTRACT
Four Göttinger miniature pigs and five domestic pigs (Landrace) of similar body weight fitted with an ileocaecal re-entrant cannula were given each a cereal-based diets with or without fish meal (Treatments 1 and 2). The diets contained increasing levels of partially hydrolysed straw meal and pectin (2:1 w/w). On day 3, 5 and 7 after the last administration of 15NH4Cl given with the diets during five days the ileal flow of endogenous nitrogen (N) was measured using the atom %15N excess in urinary N as indicator for that in endogenous N. The 15N-enrichments in urinary N, in trichloroacetic acid (TCA)-soluble N of blood plasma as well as in TCA-soluble and -preciptable N in the pancreas and small intestine were nearly the same. Furthermore, the contribution of endogenous N to total, TCA-preciptable and bacterial N of ileal digesta was not affected by the three collection periods. Urinary N seems to be the easiest accessible and valid indicator for determination of the endogenous N under these experimental conditions. Neither the fibre supplements nor the N intake affected the daily ileal flow of endogenous N. Contrary to total bacterial N , the ileal flow of bacterial N of endogenous origin was not affected by the level of protein intake. The contribution of endogenous N associated with bacteria to total endogenous N ranged between 0.43 to 0.56 (Treatment 1) and 0.40 to 0.53 (Treatment 2). On the other side, the proportion of endogenous N in bacterial N increased from 0.48 to 0.57 (treatment 1) up to 0.56 to 0.79 (Treatment 2). It is concluded that endogenous N represents an easily available N source for bacterial protein synthesis at both levels of N intake.
 
CITATIONS (8):
1. Effect of dietary carbadox or formic acid and fibre level on ileal and faecal nutrient digestibility and microbial metabolite concentrations in ileal digesta of the pig
K Partanen, T Jalava, J Valaja, S Perttilä, H Siljander-Rasi, H Lindeberg
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W. Drochner, A. Kerler, B. Zacharias
Journal of Animal Physiology and Animal Nutrition
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U. Hennig, C. C. Metges, A. Berk, A. Tuchscherer, M. Kwella
Journal of Animal Science
4. A Casein Hydrolysate Does Not Enhance Gut Endogenous Protein Flows Compared with Intact Casein When Fed to Growing Rats
Amélie Deglaire, Paul J. Moughan, Cécile Bos, Klaus Petzke, Shane M. Rutherfurd, Daniel Tomé
The Journal of Nutrition
5. Apparent Precaecal Digestibility of Nutrients and Level of Endogenous Nitrogen in Digesta of the Small Intestine of Growing Pigs as Affected by Various Digesta Viscosities
J. Bartelt, Anke Jadamus, Frigga Wiese, Ewa Swiech, Lucyna Buraczewska, O. Simon
Archiv für Tierernaehrung
6. Effect of Dietary Fiber on Endogenous Nitrogen Flows in Lactating Dairy Cows
D.R. Ouellet, M. Demers, G. Zuur, G.E. Lobley, J.R. Seoane, J.V. Nolan, H. Lapierre
Journal of Dairy Science
7. Effects of a dietary organic acid mixture and of dietary fibre levels on ileal and faecal nutrient apparent digestibility, bacterial nitrogen flow, microbial metabolite concentrations and rate of passage in the digestive tract of pigs
K. Partanen, T. Jalava, J. Valaja
animal
8. Nutritional and Physiological Functions of Amino Acids in Pigs
Paul Moughan, Warren Miner-Williams
ISSN:1230-1388