ORIGINAL PAPER
Detection of transgenic maize Cry1Ab protein
subjected to ruminal digestion
| 1 | Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN 55108, USA |
| 2 | Department of Food Science and Nutrition, University of Minnesota, St. Paul, MN 55108, USA |
| 3 | Department of Animal Science, University of Minnesota, St. Paul, MN 55108, USA |
CORRESPONDING AUTHOR
F. Diez-Gonzalez
Department of Food Science and Nutrition, University of Minnesota, St. Paul, MN 55108, USA
Department of Food Science and Nutrition, University of Minnesota, St. Paul, MN 55108, USA
Publication date: 2005-10-17
J. Anim. Feed Sci. 2005;14(4):655–664
KEYWORDS
ABSTRACT
This research determined the extent of ruminal degradation of a transgenic protein (Cry1Ab)
expressed in kernels of maize (Zea mays) cultivar DKC53-32. Cracked kernels of transgenic maize
(Bt+) were subjected to rumen digestion using in situ experiments with two ruminally cannulated
Holstein cows. A non-transgenic maize cultivar (Bt-) was used as a control for crude protein (CP)
degradation. CP and Cry1A concentrations from dacron polyester bags inserted in the rumen were
determined for 48 h. The Bt- cultivar had greater CP content (P<0.05) than the Bt+ maize, but
no difference (P>0.05) was found in ruminal degradable protein and the CP rate of degradation.
The Cry1Ab protein was detected throughout ruminal digestion. The amount of Cry1Ab protein
remaining after 16 and 24 h of ruminal degradation was 85 and 60%, respectively. These results
indicated that a significant fraction of Cry1Ab protein was not digested in the rumen. This is the first
report of the rumen degradation rate of a transgenic protein.
CITATIONS (1):
1.
Pepsin Degradation of Cry1A(b) Protein Purified from Genetically Modified Maize (Zea mays)
Luis de, María Lavilla, Lourdes Sánchez, Miguel Calvo, María Pérez
Journal of Agricultural and Food Chemistry
Luis de, María Lavilla, Lourdes Sánchez, Miguel Calvo, María Pérez
Journal of Agricultural and Food Chemistry
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