ORIGINAL PAPER
Detection of transgenic maize Cry1Ab protein subjected to ruminal digestion
 
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1
Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN 55108, USA
2
Department of Food Science and Nutrition, University of Minnesota, St. Paul, MN 55108, USA
3
Department of Animal Science, University of Minnesota, St. Paul, MN 55108, USA
CORRESPONDING AUTHOR
F. Diez-Gonzalez   

Department of Food Science and Nutrition, University of Minnesota, St. Paul, MN 55108, USA
Publication date: 2005-10-17
 
J. Anim. Feed Sci. 2005;14(4):655–664
 
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ABSTRACT
This research determined the extent of ruminal degradation of a transgenic protein (Cry1Ab) expressed in kernels of maize (Zea mays) cultivar DKC53-32. Cracked kernels of transgenic maize (Bt+) were subjected to rumen digestion using in situ experiments with two ruminally cannulated Holstein cows. A non-transgenic maize cultivar (Bt-) was used as a control for crude protein (CP) degradation. CP and Cry1A concentrations from dacron polyester bags inserted in the rumen were determined for 48 h. The Bt- cultivar had greater CP content (P<0.05) than the Bt+ maize, but no difference (P>0.05) was found in ruminal degradable protein and the CP rate of degradation. The Cry1Ab protein was detected throughout ruminal digestion. The amount of Cry1Ab protein remaining after 16 and 24 h of ruminal degradation was 85 and 60%, respectively. These results indicated that a significant fraction of Cry1Ab protein was not digested in the rumen. This is the first report of the rumen degradation rate of a transgenic protein.
 
CITATIONS (1):
1.
Pepsin Degradation of Cry1A(b) Protein Purified from Genetically Modified Maize (Zea mays)
Luis de, María Lavilla, Lourdes Sánchez, Miguel Calvo, María Pérez
Journal of Agricultural and Food Chemistry
 
ISSN:1230-1388