The study was conducted on 90 Isa Brown hens aged 28 weeks, randomly allocated to 6 groups of 15 birds each and maintained in individual laying cages throughout 12 weeks of the experiment. Hens of control group were fed a standard diet containing 19 g crude fat per kg, diets for the experimental groups contained 38 g crude fat per kg due to enrichment of the formula with 50 g of regular linseed cv. Opal or Omega (HA), low α-linolenic linseed cv. Linola (LA), 20 g of LA linseed oil, or 20 g of soyabean oil. The increase in the dietary fat content in experimental groups decreased feed intake by 5% (P < 0.05), increased egg production by 4% (P < 0.05) except in the group with LA oil, and decreased egg weight by 2% on average (P < 0.05), in comparison with the control group. The total cholesterol content averaged 284 mg per egg in groups fed LA linseed or soyabean oil and in the control group, while in the groups fed LA linseed and LA oil, it averaged 291 mg per egg (P < 0.05). In conclusion, linseed cultivars with a high content of linoleic acid cannot be considered useful in production of functional eggs.