The experiment evaluated the impact of graded levels (0, 2.5, 5%) of dried stinging nettle (SN) leaves on dietary apparent metabolizable energy (AME), n-corrected AME (AMEn), dry matter (DMR), nitrogen (NR), fat (FR) retentions and neutral detergent fibres digestibility (NDFD), without and with exogenous carbohydrase enzymes (XYL). Six experimental diets were formulated and fed to seventy-two Hy-Line Brown hens from 50 to 52 weeks of age. Each diet was fed to six enriched laying hen cages, two birds in a cage, following randomisation. The SN contained 351 g/kg neutral detergent fibre (NDF), and its inclusion linearly decreased (P < 0.05) the AMEn and FR (P < 0.001) and tended (P < 0.01) to reduce AME and DMR in diets. Exogenous XYL supplementation increased AME, AMEn, DMR, NR and NDFD (P < 0.05) and FR (P < 0.001) in diets. Each g of SN was predicted to reduce dietary AME and AMEn by 6.7 and 6.5 J, respectively, although XYL supplementation increased them by 0.36 and 0.30 MJ (P < 0.05), respectively. Therefore, supplementing SN enriched diets (especially over 2.5%) with XYL may be a strategy to mitigate the reduction in available energy due to higher dietary fibre content, and to increase nutrient availability in laying hen diets.