ORIGINAL PAPER
Figure from article: Effect of dietary...
 
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ABSTRACT
Determining the dietary requirements of sulphur containing amino acids (SAA), particularly methionine (Met), is critical for formulating nutritionally balanced poultry diets. The objective of this study was to evaluate the effects of different dietary Met levels on growth, production and egg quality of laying hens from 65 to 72 weeks of age. A total of 480 Hy-Line Brown laying hens were randomly assigned to six dietary groups for eight weeks. The treatments consisted of diets formulated to provide 90, 95, 100, 105, 110, and 120% o of the SAA levels recommended by the National Research Council (NRC), with 100% SAA serving as the control (CON). Growth was recorded at the beginning and at weeks 2, 4, 6, and 8, while egg production and feed intake data were measured weekly. Egg quality parameters were recorded at weeks 2, 4, 6, and 8. Supplementation with 90, 95, and 120% SAA significantly reduced egg production, egg weight, and feed conversion ratio (FCR), while 105 and 110% SAA levels were comparable to the CON. Egg quality traits, including Haugh unit, eggshell strength, and eggshell thickness were unaffected by the dietary treatments. These findings demonstrate that decreasing Met to levels below NRC recommendations, or increasing it by more than 10%, negatively affects production performance. The NRC-recommended dose is therefore appropriate for Hy-Line Brown laying hens at 64 weeks of age.
FUNDING
The present research was supported by the research fund of Dankook University in 2025.
CONFLICT OF INTEREST
The Authors declare that there is no conflict of interest.
REFERENCES (32)
1.
Austic R.E. (Editor), 1986. Nutrient Requirements of Poultry and Nutritional Research. Fisher, and Boorman. Ed Butterworths. London (UK).
 
2.
Brede A., Wecke C., Liebert F., 2018. Does the optimal dietary methionine to cysteine ratio in diets for growing chickens respond to high inclusion rates of insect meal from Hermetia illucens? Animals. 8, 187, https://doi.org/10.3390/ani811....
 
3.
Bregendahl K., Roberts S.A., Kerr B., Hoehler D., 2008. Ideal ratios of isoleucine, methionine, methionine plus cystine, threonine, tryptophan, and valine relative to lysine for white leghorn-type laying hens of twenty-eight to thirty-four weeks of age. Poult. Sci. 87, 744–758, https://doi.org/10.3382/ps.200....
 
4.
Brumano G., Gomes P.C., Donzele J.L., Santiago H., da Rostagno T.C.R., de Almeida R.L., 2010. Digestible methionine + cystine level in meals for light-weight laying hens from 24 to 40 weeks of age. Rev. Bras. Zootec. 39, 1228–1236, https://doi.org/10.1590/S1516-....
 
5.
Bunchasak C., Silapsorn T., 2005. Effects of adding methionine in low-protein diet on production performance, reproductive organs and chemical liver composition of laying hens under tropical conditions. Inter. J. Poult. Sci. 4, 301–308, https://doi.org/10.3923/ijps.2....
 
6.
Carvalho T.S.M, Sousa L.S., Nogueira F.A., Vaz D.P., Saldanha M.M., Triginelli M.V., Pinto M.F.V.S, Baiao N.C., Lara L.J.C., 2018. Digestible methionine + cysteine in the diet of commercial layers and its influence on the performance, quality, and amino acid profile of eggs and economic evaluation. Poult. Sci. 97, 2044–2052, https://doi.org/10.3382/ps/pey....
 
7.
Castro F.L.S., Kim H.Y., Hong Y.G., Kim W.K., 2019. The effect of total sulfur amino acid levels on growth performance, egg quality, and bone metabolism in laying hens subjected to high environmental temperature. Poult. Sci. 98, 4982–4993, https://doi.org/10.3382/ps/pez....
 
8.
Dersjant-Li Y., Peisker M., 2011. A review on recent findings on amino acids requirements in poultry studies. Iran. J. Appl. Anim. Sci. 1, 73–79.
 
9.
Dozier W.A., Kidd M.T., Corzo A., 2008. Dietary amino acid responses of broiler chickens. J. Appl. Poult. Res. 17, 157–167, https://doi.org/10.3382/japr.2....
 
10.
Gunawardana P., Roland D., Bryant M., 2008. Effect of energy and protein on performance, egg components, egg solids, egg quality, and profits in molted Hy-Line W-36 hens. J. Appl. Poult. Res. 17, 432–439, https://doi.org/10.3382/japr.2....
 
11.
Harms R., Hinton K., Russell G., 1999. Energy: methio nine ratio and formulating feed for commercial layers. J. Appl. Poult. Res. 8, 272–279, https://doi.org/10.1093/japr/8....
 
12.
Harms R.H., Russell G.B., 1996. A re-evaluation of the methionine requirement of the commercial layer. J. Appl. Anim. Res. 9, 141–151, https://doi.org/10.1080/097121....
 
13.
Harper A.E., Benevenga N.J., Wohlhueter R.M., 1970. Effects of ingestion of disproportionate amounts of amino acids. Physiol. Rev. 50, 428–558, https://doi.org/10.1152/physre....
 
14.
Ji F., Fu S.Y., Ren B., Wu S.G., Zhang H.J., Yue H.Y., Gao J., Helmbrecht A., Qi G.H., 2014. Evaluation of amino-acid supplemented diets varying in protein levels for laying hens. Appl. Poult. Sci. 23, 384–392, https://doi.org/10.3382/japr.2....
 
15.
Jordao F.J., Silva J.H.V., Silva E.L., Ribeiro G.M.L., Martins T.D.D., Rabello C.B., 2006. Methionine + cystine requirements of semi-heavy laying hens from the starter to peak of egg production. Rev. Bras. de Zootec. 35, 1063–1069, https://doi.org/10.1590/S1516-....
 
16.
Kakhki R.A.M., Golian A., Zarghi H., 2016. Effect of digestible methionine + cystine concentration of performance, egg quality and blood metabolites in laying hens. British Poult. Sci. 57, 403–414, https://doi.org/10.1080/000716....
 
17.
Leeson S., Summers J.D. (Editors), 2001. Nutrition of the Chicken. University Books. Guelph (Canada).
 
18.
Liu G., Sharma M.K., Tompkins Y.H., Teng P.Y., Kim W.K., 2024. Impacts of varying methionine to cysteine supplementation ratios on growth performance, oxidative status, intestinal health, and gene expression of immune response and methionine metabolism in broilers under Eimeria spp. challenge. Poult. Sci. 130, 103300, https://doi.org/10.1016/j.psj.....
 
19.
Liu Z., Wu G., Bryant M., Roland D., 2005. Influence of added synthetic lysine in low-protein diets with the methionine plus cysteine to lysine ratio maintained at 0.75. J. Appl. Poult. Res. 14, 174–182, https://doi.org/10.1093/japr/1....
 
20.
Narvaez-Solarte W.N., Rostagno H.S., Soares P.R., Silva M.A., Velasquez L.F.U., 2005. Nutritional requirements in methionine + cystine for white-egg laying hens during the first cycle of production. Int. J. Poult. Sci. 18, 965–968, https://doi.org/10.3923/ijps.2....
 
21.
Novak C., Yakout H., Scheideler S., 2004. The combined effects of dietary lysine and total sulfur amino acid level on egg production parameters and egg components in DEKALB delta laying hens. Poult. Sci. 83, 977–984, https://doi.org/10.1093/ps/83.....
 
22.
NRC., 1994. Nutrient Requirements of Poultry. National Academy of Sciences. Washington, DC.
 
23.
Rao S.V.R., Ravindran V., Srilatha T., Panda A.K., Raju A.M.V.L.N., 2011. Effect of dietary concentrations of energy, crude protein, lysine, and methionine on the performance of White Leghorn layers in the tropics. Appl. Poult. Sci. 20, 528–541, https://doi.org/10.3382/japr.2....
 
24.
Safaa H.M., Serrano M.P., Valencia D.G., Arbe X., Jimenez M.E., Lazaro R., Mateos G.G., 2008. Effects of the levels of methionine, linoleic acid, and added fat in the diet on productive performance and egg quality of brown laying hens in the late phase of production. Poult. Sci. 87, 1595–1602, https://doi.org/10.3382/ps.200....
 
25.
Santana M.H.M., Costa F.G.P., Guerra R.R., Givisiez P.E.N., Junior J.P.F., Lima M.R., 2021. Methionine plus cystine levels for light laying hens on production performance. Res. Soc. Develop. 10, 57910212817, https://doi.org/10.33448/rsd-v....
 
26.
Schutte J., Jong J.D., Bertram H.L., 1994. Requirement of the laying hen for sulfur amino acids. Poult. Sci. 73, 274–280, https://doi.org/10.3382/ps.073....
 
27.
Schutte J., Weerden V.E., Bertram H., 1983. Sulphur amino acid requirement of laying hens and the effects of excess dietary methionine on laying performance. Brit. Poult. Sci. 24, 319–326, https://doi.org/10.1080/000716....
 
28.
Schutte J.B., Weerden V.E., 1978. Requirement of the hen for sulphur-containing amino acids. Brit. Poult. Sci. 19, 573–581, https://doi.org/10.1080/000716....
 
29.
Shafer D.J., Carey J.B., Prochaska J.F., 1996. Effect of dietary methionine intake on egg component yield and composition. Poult. Sci. 75, 1080–1085, https://doi.org/10.3382/ps.075....
 
30.
Shafer D.J., Carey J.B., Prochaska J.F., Sams A.R., 1998. Dietary methionine intake effects on egg component yield, com position, functionality, and texture profile analysis. Poult. Sci. 77, 1056–1062, https://doi.org/10.1093/ps/77.....
 
31.
Sohail S.S., Bryant M.M., Roland D.A., 2002. Influence of supplemental lysine, isoleucine, threonine, tryptophan and total sulfur amino acids on egg weight of Hy-Line W-36 hens. Poult. Sci. 81, 1038–1044, https://doi.org/10.1093/ps/81.....
 
32.
Waldroup P., Hellwig H., 1995. Methionine and total sulfur amino acid requirements influenced by stage of production J. Appl. Poult. Res. 4, 283–292, https://doi.org/10.1093/japr/4....
 
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