ORIGINAL PAPER
 
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ABSTRACT
The in vitro ruminal degradation kinetics of dry matter (DM) and crude protein (CP) were determined in alfalfa silages treated with different doses of dry ice (0, 0.5, 1, and 2 g 750 g−1 fresh matter (FM)) and varying wilting time (0, 12, and 24 h). Ruminal fluid was collected from five rumen-cannulated sheep. Feed samples were placed in labelled filter bags and incubated for 0, 2, 4, 8, 24, and 48 h. The lowest ruminal DM degradation was observed in silages subjected to a 12-h wilt with the addition of 0.5 and/or 2 g of dry ice (66 and 65%, respectively). The final CP degradability was the lowest in silages prepared after 12-h and 24-h wilts compared to silages made from unwilted alfalfa. After 48 h of incubation, CP degradability in unwilted silages treated with 0, 0.5, 1, and 2 g of dry ice reached 77, 79, 76, and 70%, respectively. The corresponding CP degradability values in silages subjected to a 12-h wilt were 73, 76, 69, and 67%, and 73, 73, 71, and 68% in silages wilted for 24 h. In vitro degradability and degradation kinetics of the analysed nutrients were the highest in silages produced after a 24-h wilt with the addition of 1 and/or 2 g of dry ice. These experimental treatments may potentially improve the efficiency of CP utilisation from alfalfa silage and increase CP availability in subsequent segments of the digestive tract.
FUNDING
This research was funded in whole or in part by the National Science Centre, Poland grant No. 2021/41/N/NZ9/01881. To ensure Open Access, the author has applied a CC-BY public copyright licence to each version of Author Accepted Manuscript (AAM) resulting from this submission.
CONFLICT OF INTEREST
The Authors declare that there is no conflict of interest.
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