0.857
IF5
0.900
IF
Q3
JCR
0.92
CiteScore
0.405
SJR
Q2
SJR
20
MNiSW
165.24
ICV
SHORT COMMUNICATION
 
CC-BY 4.0
 
 

The profile of gene expression during bovine adipogenesis: Cloning and expression of type XII collagen isoforms

H. Aso 1  ,  
K. Tahara 2,  
T. Yamasaki 2,  
M. T. Rose 3,  
T. Kido 1,  
T. Minashima 1,  
K. Miyazawa 1,  
S. Hayashi 1,  
M. Sanosaka 1,  
K. Watanabe 1,  
S. Ohwada 1,  
 
1
Cellular Biology Laboratory, Tohoku University, Sendai, 981-8555, Japan
2
Department of Advanced Medicine and Development, BML Inc., Saitama, 350-1101, Japan
3
Institute of Rural Sciences, University of Walesm Aberystwyth, Ceredigion, SY23 3AL, UK
J. Anim. Feed Sci. 2004;13(Suppl. 1):369–372
Publish date: 2004-08-30
KEYWORDS
ABSTRACT
To isolate candidate genes involved in bovine adipocyte differentiation, we have constructed a subtraction library from a clonal bovine intramuscular preadipocyte (BIP) cell line using PCRsubtraction. We have isolated a set of subtracted cDNA fragments whose respective mRNA levels are up regulated during adipogenesis. Two cDNA clones were determined to be type XII collagen alpha-1. From the expression analysis of type XII collagen, the XIIA-2 isoform was mainly expressed in differentiated BIP cells and adipose tissues. These results suggest that type XII collagen may be associated with adipocyte differentiation and adipose formation in cattle and is a potentially useful marker for adipogenesis.
CORRESPONDING AUTHOR
H. Aso   
Cellular Biology Laboratory, Tohoku University, Sendai, 981-8555, Japan
ISSN:1230-1388