0.857
IF5
0.900
IF
Q3
JCR
0.92
CiteScore
0.405
SJR
Q2
SJR
20
MNiSW
165.24
ICV
SHORT COMMUNICATION
 
CC-BY 4.0
 
 

Establishment of mammary tissue culture: an in vitro model for bovine lactation

H. H. Wu 1,  
X. J. Zhao 1,  
C. Q. Zhang 1,  
J. X. Liu 1  
 
1
Zhejiang University, College of Animal Sciences, Hangzhou 310029, P.R.China
J. Anim. Feed Sci. 2004;13(Suppl. 1):575–578
Publish date: 2004-08-30
KEYWORDS
ABSTRACT
A ruminant mammary tissue culture that accurately reproduces mammary function in vitro would be a valuable tool in studies of ruminant lactation. This study was conducted to establish an in vitro model for culture of lactating bovine mammary tissue. The tissue still maintained the activity at 72 h, which was indicated by the value of MTT and oligonucleosomal fraction of cellular DNA. Under the conditions used in this study, the gene expression of αs1 casein was highest at 8 h, and then declined at 12 and 24 h. The results suggest that this primary tissue culture system may be useful in studying the effects of different combinations of amino acids on milk protein in terms of αs1 casein in vitro.
CORRESPONDING AUTHOR
J. X. Liu   
Zhejiang University, College of Animal Sciences, Hangzhou 310029, P.R.China
 
CITATIONS (2):
1. Methionine protects against hyperthermia-induced cell injury in cultured bovine mammary epithelial cells
Zhao-Yu Han, Tian Mu, Zhen Yang
Cell Stress and Chaperones
2. Establishment and characterization of a lactating bovine mammary epithelial cell model for the study of milk synthesis
Ke Zhao, Hong‑Yun Liu, Miao‑Miao Zhou, Jian‑Xin Liu
Cell Biology International
ISSN:1230-1388