ORIGINAL PAPER
The influence of inflammatory process on prostaglandin F2α contractile activity in porcine uterus
J. Kucharski 1  
,   J. Jaroszewski 2,   B. Jana 1,   J. Górska 1,   A. Kozłowska 1,   W. Markiewicz 2
 
More details
Hide details
1
Division of Reproductive Endocrinology and Pathophysiology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland
2
Division of Pharmacology, Department of Pathology and Pharmacology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 5, 10-718 Olsztyn, Poland
CORRESPONDING AUTHOR
J. Kucharski   

Division of Reproductive Endocrinology and Pathophysiology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland
Publication date: 2007-11-21
 
J. Anim. Feed Sci. 2007;16(4):654–667
 
KEYWORDS
ABSTRACT
The aim of this study was to determine the influence of prostaglandin F2α (PGF2α) on the contractile activity of intact and inflamed porcine uteri. On day 3 of the oestrous cycle, gilts were randomly assigned to group I (control; n = 6) or II (treated with E. coli; n = 6). In all gilts median laparotomy was performed and 50 ml of saline or 50 ml of E. coli containing 109 colony-forming units/ml were injected into each uterine horn in groups I and II, respectively. After seven days (on day 10 of the oestrous cycle) the gilts were slaughtered and uterine horns collected. After macroscopic examination, contractile activity of uterine strips: endometrium+myometrium (ENDO/MYO) and myometrium (MYO) was examined using noradrenaline (NA), acetylocholine (ACh), PGF2α alone or together with EP2,EP4, EP1, EP3 receptor blockers (BEP2, BEP4, BEP1 and BEP3, respectively). NA decreased (P<0.01, P<0.001) the intensity of contractions in ENDO/MYO and MYO of the control group but increased (P<0.05, P<0.01) in the inflamed uterus. ACh at doses 10-5, 10-4 and 10-3 M increased (P<0.05-0.001) the intensity of contractions in ENDO/MYO and MYO from the bacteria group and intact group (P<0.05) at doses10-4 and 10-3 M. ACh at all of the tested doses increased (P<0.05-0.001) the frequency of contractions in the control and decreased (P<0.05, P<0.001) in the experimental group. NA and ACh treatment revealed that uterine strips were living and suitable for further investigations. PGF2α at a dose of 10-7 M increased (P<0.05, P<0.01) the intensity of contractions in ENDO/MYO and MYO in both control and experimental groups. PGF2α at doses 10-8 and 10-7 M used in the presence of BEP2 or BEP4 increased (P<0.05-0.001) the intensity of contrac- tions in both the control and bacterial groups. However, PGF2α did not significantly influence the frequency of contractions with the exception of the stimulatory effect in ENDO/MYO (P<0.001) strips of the control groups. PGF2α at doses of 10-8 and 10-7 M administered after BEP1 and BEP3 treatment decreased (P<0.05-0.001) or did not significantly influence the intensity and frequency of contractions in uterine tissues in both examined groups. The presented findings revealed that PGF2α, besides affecting the contractile activity of uterine tissues, may also act through the PGE2 receptors. However, thorough interpretation of these results requires determination of the role of other prostanoids in this process.
 
CITATIONS (11):
1.
The influence of leukotrienes C4 and D4 on the contractility of an inflamed porcine uterus
B. Jana, J.J. Jaroszewski, J. Czarzasta, W. Markiewicz
Theriogenology
 
2.
Synthesis of prostacyclin and its effect on the contractile activity of the inflamed porcine uterus
B. Jana, J.J. Jaroszewski, J. Czarzasta, M. Włodarczyk, W. Markiewicz
Theriogenology
 
3.
Comparison of the porcine uterine smooth muscle contractility on days 12–14 of the estrous cycle and pregnancy
Włodzimierz Markiewicz, Marek Bogacki, Michał Blitek, Jerzy Jan Jaroszewski
Acta Veterinaria Scandinavica
 
4.
β2– and β3–adrenergic receptors stimulation relaxes porcine myometrium in the peri-implantation period1,2
W. Markiewicz, J. J. Jaroszewski
Journal of Animal Science
 
5.
Influence of β2- and β3-adrenoceptor agonists on contractile activity of the porcine myometrium in the luteal phase and the first days of pregnancy
W. Markiewicz, J.J. Jaroszewski
Polish Journal of Veterinary Sciences
 
6.
Participation of analogues of lysophosphatidic acid (LPA): oleoyl-sn-glycero-3-phosphate (L-α-LPA) and 1-oleoyl-2-O-methyl-rac-glycerophosphothionate (OMPT) in uterine smooth muscle contractility of the pregnant pigs
W. Markiewicz, K. Kamińska, M. Bogacki, T. Maślanka, J. Jaroszewski
Polish Journal of Veterinary Sciences
 
7.
Participation of Prostaglandin E2 in Contractile Activity of Inflamed Porcine Uterus
Barbara Jana, Jerzy Jaroszewski, Jan Kucharski, Marlena Koszykowska, Jolanta Górska, Włodzimierz Markiewicz
Acta Veterinaria Brno
 
8.
Participation of acetylcholine and its receptors in the contractility of inflamed porcine uterus
B. Jana, J. Całka, M. Bulc, K.K. Piotrowska-Tomala
Theriogenology
 
9.
Inflammation changes the expression of neuropeptide Y receptors in the pig myometrium and their role in the uterine contractility
Barbara Jana, Jarosław Całka, Katarzyna Palus, Rudolf Kirchmair
PLOS ONE
 
10.
The role of somatostatin and its receptors (sstr2, sstr5) in the contractility of gilt inflamed uterus
Barbara Jana, Jarosław Całka, Marta Czajkowska
Research in Veterinary Science
 
11.
Alterations in the relative abundance of the vasoactive intestinal peptide receptors (VPAC1 and VPAC2) and functions in uterine contractility during inflammation
Katarzyna Palus, Jarosław Całka, Barbara Jana
Animal Reproduction Science
 
ISSN:1230-1388