0.857
IF5
0.900
IF
Q3
JCR
0.92
CiteScore
0.405
SJR
Q2
SJR
20
MNiSW
165.24
ICV
ORIGINAL PAPER
 
CC-BY 4.0
 
 

The Toll-like receptors mRNA expression profile in the pineal gland of sheep during long and short days

J. Bochenek 1,  
J. Skipor 2,  
M. Kowalewska 2,  
A. P. Herman 1  
 
1
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
2
Institute of Animal Reproduction and Food Research, Polish Academy of Sciences,Tuwima 10, 10-748 Olsztyn, Poland
J. Anim. Feed Sci. 2015;24(3):208–215
Publish date: 2015-09-08
KEYWORDS
ABSTRACT
Interaction between the immune system and pineal gland seems to be bidirectional. The feedback effect of an inflammatory response on the pineal gland is poorly understood, however. This study was performed to analyse the pattern of mRNA expression of receptors belonging to the Toll-like receptors (TLR) family in the pineal gland of ewes under different photoperiod conditions. TLRs are involved in the initiation of the innate immune response because they recognize different pathogen-associated molecular patterns (PAMPs). The experiment was carried out on adult Blackface ewes during long-day (LD, 18:6, June; n = 6) and short-day (SD, 6:18, December; n = 6) periods. The animals were euthanized 5 h after sunset (at 21:00 during SD and 2:00 during LD). The mean concentration of circulating melatonin in the last hour of the experiment was higher (P < 0.05) during the SD (270 ± 15 pg· ml–1) compared with the LD period (108 ± 5 pg · ml–1). The expression of genes encoding each TLR was differentiated. TLR3 had the highest mRNA expression, followed by TLRs 1, 2, 5, 7, 9, 8, 4, 10 and 6. Gene expression also varied for each TLR and was influenced by the photoperiod and/or melatonin. The gene whose expression varied the most was TLR10 (coefficient of variation, 42%) during the LD period, but during the SD period, the expression of TLR6 varied most (coefficient of variation, 42%). The study showed that the cells of the pineal gland express transcripts for all ten members of the TLR family, therefore, the functioning of this gland may be affected by number of PAMPs recognized by these receptors
CORRESPONDING AUTHOR
A. P. Herman   
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
 
CITATIONS (5):
1. Central Interleukin-1βSuppresses the Nocturnal Secretion of Melatonin
A. P. Herman, J. Bochenek, K. Król, A. Krawczyńska, H. Antushevich, B. Pawlina, A. Herman, K. Romanowicz, D. Tomaszewska-Zaremba
Mediators of Inflammation
2. Endotoxin-induced inflammation disturbs melatonin secretion in ewe
Andrzej Przemysław Herman, Karolina Wojtulewicz, Joanna Bochenek, Agata Krawczyńska, Hanna Antushevich, Bartosz Pawlina, Marlena Zielińska-Górska, Anna Herman, Katarzyna Romanowicz, Dorota Tomaszewska-Zaremba
Asian-Australasian Journal of Animal Sciences
3. Effect of CD14/TLR4 antagonist on GnRH/LH secretion in ewe during central inflammation induced by intracerebroventricular administration of LPS
Karolina Haziak, Andrzej Przemysław Herman, Karolina Wojtulewicz, Bartosz Pawlina, Kamila Paczesna, Joanna Bochenek, Dorota Tomaszewska-Zaremba
Journal of Animal Science and Biotechnology
4. Inflammation and LPS-Binding Protein Enable the Stimulatory Effect of Endotoxin on Prolactin Secretion in the Ovine Anterior Pituitary: Ex Vivo Study
Dorota Tomaszewska-Zaremba, Karolina Haziak, Monika Tomczyk, Andrzej P. Herman
Mediators of Inflammation
5. Melatonin, a toll-like receptor inhibitor: Current status and future perspectives
Omid Reza Tamtaji, Moein Mobini, Russel J. Reiter, Abolfazl Azami, Mohammad Saeed Gholami, Zatollah Asemi
Journal of Cellular Physiology
ISSN:1230-1388