ORIGINAL PAPER
Rumen and total intestinal tract digestibility of protein and amino acids from different roughages, determined in situ
 
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1
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
2
The National Institute of Animal Science, Research Centre Foulum, P.O. Box 39, DK-8830 Tjele, Denmark
Publication date: 1996-09-13
 
J. Anim. Feed Sci. 1996;5(4):347–363
 
KEYWORDS
ABSTRACT
Eight different roughages (clover grass silage, 2 samples of fresh clover grass, fresh ryegrass, winter wheat whole crop silage, pea whole crop silage, dried grass and goats rue) were examined for effective degradability in the rumen and total tract digestibility of individual amino acids (AA), total AA and crude protein using in situ method. Effective degradabilities calculated at a rumen fractional outflow rate of 5%/h, were higher for crude protein than for total AA (the differences ranging from 1.9 to 4.1 percentage points). Arginine was the least degradable amino acid, particularly in silage and in short rumen incubations, although cysteine and phenylalanine also tended to be less degraded than total AA. The most degradable amino acids were alanine and proline in silages, and cysteine, aspartic acid and glutamic acid in the other roughages. Disappearance of total AA from mobile bags containing samples of feeds after 16 h preincubation in the rumen varied from 79.8% in dried grass to 95.9% in fresh clover grass, and was generally similar to the disappearance of crude protein, 79.7-96.3%, respectively. In most of the examined roughages, cystine and glycine disappeared the least, and glutamic acid, aspartic acid and alanine the most from mobile bags, as compared with total AA.
 
CITATIONS (11):
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Ideal amino acid profiles as a basis for feed protein evaluation
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Profile of digested feed amino acids from untreated and expander treated feeds estimated using in situ methods in dairy cows
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Amino acid profile of metabolisable protein in lactating dairy cows is affected by dry matter concentration in grass-clover silage
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Utilisation by ruminants of nitrogen compounds in silage-based diets
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Ruminal degradability of15N labelled ribonucleic acid in grass
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In vitro techniques to replace in vivo methods for estimating amino acid supply
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11.
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ISSN:1230-1388