0.857
IF5
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IF
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JCR
0.92
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MNiSW
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ICV
ORIGINAL PAPER
 
CC-BY 4.0
 
 

Molecular characterization, sequence variation and association with fat deposition traits of ACOX1 gene in pigs

B. Zuo 1,  
H. Yang 1,  
J. Wang 1,  
M. G. Lei 1,  
Y. Z. Xiong 1  
 
1
Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture & Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, P.R. China
J. Anim. Feed Sci. 2007;16(3):433–444
Publish date: 2007-09-06
KEYWORDS
ABSTRACT
Preoxisomal acyl-CoA oxidase 1 (ACOX1) catalyses the beta-oxidation of very long chain fatty acids and thus plays an essential role in fatty acid degradation. The objective of this study was to clone a gene encoding ACOX1 and to characterize its association with fat deposition in pigs. The complete coding sequence was 1986bp and encoded a protein of 661 amino acids including the carboxyl-terminal sequence (Ser-Lys-Leu) known as a minimal peroxisome-targeting signal. The predicted porcine ACOX1 amino acid sequence shared 88, 84, 81, 86 and 78% identity to that of human, mouse, rat, bovine and chicken counterparts, respectively. The A/C polymorphism in intron 9 was detected by PCR-PstI-RFLP and the allele frequencies of this polymorphism were significantly different between Chinese fatty type pig breeds (Meishan, Erhualian, Tongcheng and Qinping) and western commercial pig breeds (Landrace, Large White and Duroc). The association analysis between the A/C polymorphism and carcass traits in 298 Large White × Meishan F2 offspring showed that the single genotype polymorphism (SNP) genotype was significantly associated with traits related to fat deposition such as backfat thickness and carcass fat percentage. The ACOX1 gene was widely expressed in the tissues examined by semi-quantitative reverse transcription (RT) PCR, with the highly abundant in liver, kidney and fat. The present study offered preliminary evidence to further determine the role of the ACOX1 gene in pig fat deposition.
CORRESPONDING AUTHOR
Y. Z. Xiong   
Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture & Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, P.R. China
 
CITATIONS (2):
1. Molecular characterization, polymorphism of the ACOX1 gene and association with ultrasound traits in Bos taurus
Y. Jiao, L.S. Zan, Y.F. Liu, H.B. Wang
Genetics and Molecular Research
2. Detection of novel SNPs and mapping of the fatness QTL on pig chromosome 7q1.1-1.4 region
W.H. Huang, Z.X. Ma, Z.Y. Xu, Y.Z. Xiong, B. Zuo
Genetics and Molecular Research
ISSN:1230-1388