Lactic acid can be easily and precisely determined by reversed-phase high performance liquid chromatography with pre-column derivatization
More details
Hide details
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
M. Czauderna   

The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
Publication date: 2008-04-08
J. Anim. Feed Sci. 2008;17(2):268–279
Simple, sensitive and rapid methods of lactic acid (LA) quantification in the presence of other short-chain fatty acids in specimens of biological origin were developed to facilitate investigation of metabolism in body tissues and fluids of farm animals, fermentation processes, and ensiled products by reversed-phase high-performance liquid chromatography (RP-HPLC). LA and other acids in assayed samples were converted to their sodium salts (RCOONa). Derivatization of RCOONa was carried out with of a solution of 2,4’-dibromoacetophenone (DBAP) (2.2–2.4 g DBAP/100 ml of acetone or 20 g DBAP/100 ml of chloroform) and triethylamine. The reaction mixture was vigorously mixed and then reacted for 1 h at 45℃. The separation of derivatized acids was performed on a Nova Pak column (4 μm, 300 x 3.9 mm I.D., Waters) using a binary gradient elution program and photodiode detection at 259 nm. Lactic, acetic and propionic acid peaks were eluted at 11.05±0.10, 18.0±0.1 and 19.6±0.1 min, respectively, while the iso-capronic acid peak (an internal standard) at 23.1±0.1 min. The total run time of the HPLC analysis was 59 min. LA detection at 259 nm assures the excellent selectivity and high sensitivity of the proposed method; the limit of detection (LOD) and the limit of quantification (LOQ) were equal to 0.04 and 0.13 ng/ml, respectively. The use of chloroform as a solvent for DBAP in the derivatization of LA in samples followed by RP-HPLC offers the best sensitivity of LA determination in biological samples. The utility of the method was demonstrated by LA analysis in different materials such as silage, fermented dairy and vegetable products, intestinal digesta, blood and etc. The presented methods based on an inexpensive HPLC column, simple and rapid processing of samples provide accurate and sensitive analytical tools for routine determination of LA, particularly in specimens of biological origin.
Determination of fatty acid derivatives in fuel and diesel oil
Andreas Ranz, Eveline Maier, Ernst Lankmayr
Lactic acid quantitation in hand dishwashing liquid using an HILIC-UV methodology
Mark Storton, James Exarchakis, Tracy Waters, Zhigang Hao, Barry Parker, Michael Knapp
Journal of Separation Science
Evaluating Quality Indices of Pickled Garlic Based on Electrical Properties
Qian Ma, Na Yang, Yamei Jin, Juanjuan Zhao, Zhengyu Jin, Xueming Xu
Journal of Food Process Engineering
Simple on-line pretreatment of column-switching coupled with ion chromatography for the determination of lactic acid in lobaplatin
Zhendong Zhao, Jianzhong Zhu, Yanli Xie
Journal of Pharmaceutical and Biomedical Analysis
Development of a HPLC–UV method for the quantitative determination of four short-chain fatty acids and lactic acid produced by intestinal bacteria during in vitro fermentation
S. De Baere, V. Eeckhaut, M. Steppe, C. De Maesschalck, P. De Backer, F. Van Immerseel, S. Croubels
Journal of Pharmaceutical and Biomedical Analysis
Validation and application of an ultrahigh-performance liquid chromatographic-Orbitrap mass spectrometric method for the simultaneous detection and quantification of volatile and non-volatile organic acids in human faecal samples
Claudio Gardana, Cristian Del Bo’, Paolo Simonetti
Journal of Pharmaceutical and Biomedical Analysis
Development and validation of a novel derivatization method for the determination of lactate in urine and saliva by liquid chromatography with UV and fluorescence detection
Davide Pellegrini, Massimo Onor, Ilaria Degano, Emilia Bramanti
Evaluation of potential prebiotics: a review
Dayang Marshitah Bajury, Siti Maisarah Nashri, Patricia King Jie Hung, Shahrul Razid Sarbini
Food Reviews International
Organic Analysis of Environmental Samples Using Liquid Chromatography with Diode Array and Fluorescence Detectors: An Overview
Susana Garcia-Alonso, Rosa María Perez-Pastor
Critical Reviews in Analytical Chemistry
Multicommutation flow analysis system for non-enzymatic lactate determination based on light-driven photometric assay
Justyna Głowacka, Robert Koncki, Kamil Strzelak
Analytica Chimica Acta