ORIGINAL PAPER
Development and optimization of an indirect enzyme-linked immunosorbent assay for 19-nortestosterone
Zh. Tian 1,   D. H. Yu 1,   Y. Y. Zhang 2,   J. H. Guo 1,   Ch. F. Peng 1,   Zh. X. Chen 1  
,   Ch. L. Xu 1  
 
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1
Department of Food Science and Technology, Southern Yangtze University, 170 Huihe Road, WuXi 214036, Jiangsu Province, P.R. China
2
Department of Biological Engineering, Southwest Jiaotong University, Chengdu 610031, Sichuan Province, P.R. China
CORRESPONDING AUTHOR
Zh. X. Chen   

Department of Food Science and Technology, Southern Yangtze University, 170 Huihe Road, WuXi 214036, Jiangsu Province, P.R. China
Ch. L. Xu   

Department of Food Science and Technology, Southern Yangtze University, 170 Huihe Road, WuXi 214036, Jiangsu Province, P.R. China
Publication date: 2008-04-08
 
J. Anim. Feed Sci. 2008;17(2):253–267
 
KEYWORDS
ABSTRACT
A polyclonal antibody-based enzyme-linked immunosorbent assay (ELISA) has been developed, optimized and validated to measure this anabolic steroid. Influence of several physicochemical parameters, such as incubation time, ionic strength, detergent concentration and pH were selected to provide a highest sensitivity on the ELISA format. The regression equation of the final inhibition curve was: y = -0.3194x + 1.6316, R2 = 0.9927. The linear range was between 0.1 and 25 ng/ml and the IC50 was 3.5 ng/ml. The specificity was evaluated by five structurally related anabolic steroids, and none of them had significant cross-reactivity. Finally, the accuracy and precision of this assay were evaluated by means of spiked samples. The recovery was between 76.9 and 104.7%, and the variation coefficient was between 5.2 and 13.4%.
 
CITATIONS (2):
1.
Development of determination of di-n-octyl phthalate (DOP) residue by an indirect enzyme-linked immunosorbent assay
Hua Kuang, Liguang Xu, Gang Cui, Wei Ma, Chuanlai Xu
Food and Agricultural Immunology
 
2.
Development of a broad-spectrum monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay for screening of androgens in animal edible tissues
Haijiao Gao, Guyue Cheng, Hui Wang, Ting Chen, Chao Xu, Houquan Lv, Heying Zhang, Ren Hou, Yulian Wang, Dapeng Peng, Zhonghui Yuan
Microchemical Journal
 
ISSN:1230-1388