REVIEW PAPER
Estimation of protein and amino acid metabolism using [15N] and [14C] labeled tracer amino acids
 
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1
Institute for Ecological Compartible Animal Husbandry, Justus-von-Liebig-Weg 8, 18051 Rostock, Germany
2
Research Institute for the Biology of Farm Animals, Department of Nutritional Physiology "Oskar Kellner", 18059 Rostock, Germany
3
Department of Human and Animal Physiology, Agricultural University, Haarweg 10, N-6709 PJ Wageningen, The Netherlands
Publication date: 1996-07-09
 
J. Anim. Feed Sci. 1996;5(3):187–199
 
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ABSTRACT
The possibilities of estimating parameters for whole body protein metabolism (synthesis and breakdown) by the help of the end product method and a compartment model using 15N- and/or 14C labeled amino acids (AA) as tracers are demonstrated. First, the fundamentals of the method, the choice of tracer AA and the determinable parameters are described. The influence of endogenous and exogenous factors on protein synthesis and breakdown is demonstrated using selected results of tracer experiments on rats. Differences relating to animal species and the effect of age, body weight, protein intake, β-agonist administration and training, are shown in detail. The main conclusions are: - the tracer method in combination with modeling provides parameters of protein metabolism on intact animals; - the estimated data are relative values and they reflect the main processes of protein and/or AA metabolism with sufficient accuracy; - the method makes it possible to assess the influence of different endogenous and exogenous factors on the protein synthesis rate.
 
CITATIONS (3):
1.
Absorption and secretion of exogenous and endogenous N along the digestive tract and kinetic parameters of protein metabolism in growing pigs
K. Krawielitzki, F. Kreienbring, J. Kowalczyk, T. Zebrowska, R. Schadereit
Journal of Animal Physiology and Animal Nutrition
 
2.
The Influence of Feeding Frequency on Nitrogen Turnover and Whole Body Protein Synthesis in Adult Rats (Estimation using [14C]- and [15N]- Labelled Leucine)
K. Krawielitzki, V. V. A. M. Schreurs, F. Kreienbring, R. E. Koopmanschap, J. Voigt, M. Gabel
Isotopes in Environmental and Health Studies
 
3.
Body Fat Deposition Does Not Originate from Carbohydrates in Milk-Fed Calves
Joost J. G. C. van den Borne, Gerald E. Lobley, Martin W. A. Verstegen, Jane-Martine Muijlaert, Sven J. J. Alferink, Walter J. J. Gerrits
The Journal of Nutrition
 
ISSN:1230-1388