0.857
IF5
0.900
IF
Q3
JCR
0.92
CiteScore
0.405
SJR
Q2
SJR
20
MNiSW
142.18
ICV
ORIGINAL PAPER
 
CC-BY 4.0
 
 

Effects of dietary protein level and rumen-protected pantothenate on nutrient digestibility, nitrogen balance, blood metabolites and growth performance in beef calves

Q. Liu 1, 2  ,  
C. Wang 1,  
H. Q. Li 1,  
G. Guo 1,  
W. J. Huo 1,  
S. L. Zhang 1,  
Y. L. Zhang 1,  
C. A. Pei 1,  
 
1
Shanxi Agricultural University, College of Animal Sciences and Veterinary Medicines, Taigu, 030801, Shanxi Province, P.R. China
2
Animal Husbandry and Veterinary Bureau of Yuci County, Yuci, 030600, Shanxi Province, P.R. China
J. Anim. Feed Sci. 2018;27(3):202–210
Publish date: 2018-08-03
KEYWORDS:
TOPICS:
ABSTRACT:
The aim of the study was to evaluate the effects of dietary different levels of crude protein (CP) and rumen-protected pantothenate (RPP) supplementation on nutrient digestibility, nitrogen balance, blood metabolites and growth performance in beef calves. Sixty Blonde d’Aquitaine × Simmental bull calves (in average 12 months of age and 354 ± 2.4 kg of body weight) were randomly assigned to four groups with a 2 × 2 factorial arrangement. Low CP (113,7 g/kg dry matter (DM)) or high CP (133,9 g/kg DM) diets were fed without or with 72 mg RPP per kg DM. The feeding experiment lasted 100 days (10 days of adaptation and 90 days of target feeding and data collection). After feeding experiment, 4 calves per treatment (still fed the same diet) were randomly selected for collection of faeces and urine from day 111 to 120. CP × RPP interactions were not observed. DM intake, average daily gain, digestibility of DM, organic matter, CP, neutral detergent fibre and acid detergent fibre, digestible N (DN), retained N (RN), DN:N intake ratio and RN:DN ratio increased, and feed conversion ratio decreased with increasing dietary CP level or RPP supplementation. Serum total protein and albumin contents increased with increasing dietary CP level or RPP supplementation. Serum urea nitrogen increased with increasing dietary CP level, but decreased with RPP supplementation. Serum concentrations of 3-hydroxy-3-methylglutaryl- CoA synthetase, pantothenic acid, acyl carrier protein and acetyl-CoA as well as activities of pantothenate kinase and succinyl-CoA were not affected by dietary CP level, but increased with RPP supplementation. So, nutrient utilization and growth performance were improved with increasing dietary CP level or RPP supplementation in beef calves.
CORRESPONDING AUTHOR:
Q. Liu   
Shanxi Agricultural University, College of Animal Sciences and Veterinary Medicines, Taigu, 030801, Shanxi Province, P.R. China
 
REFERENCES (31):
1. AOAC International, 1995. Official Methods of Analysis of AOAC International. 16th Edition. Arlington, VA (USA).
2. Atasoglu C., Newbold C.J., Wallace R.J., 2001. Incorporation of [15N] ammonia by the cellulolytic ruminal bacteria Fibrobacter succinogenes BL2, Ruminococcus albus SY3, and Ruminococcus flavefaciens 17. Appl. Environ. Microbiol. 67, 2819–2822, https://doi.org/10.1128/AEM.67....
3. Ball G.F.M., 2006. Pantothenic acid. In: G.F.M. Ball (Editor). Vitamins in Foods: Analysis, Bioavailability and Stability. CRC Press. Boca Raton, FL (USA), pp. 211–219.
4. Bonomi A., 2000. Dairy cattle ration integration with rumen-protected pantothenic acid: effects on milk production and reproductive efficiency. Riv. Sci. dell’Aliment. 29, 321–338.
5. Chanthakhoun V., Wanapat M., Berg J., 2012. Level of crude protein in concentrate supplements influenced rumen characteristics, microbial protein synthesis and digestibility in swamp buffaloes (Bubalus bubalis). Livest. Sci. 144, 197–204, https://doi.org/10.1016/j.livs....
6. Chen S., Paengkoum P., Xia X., Na-Lumpang P., 2010. Effects of dietary protein on ruminal fermentation, nitrogen utilization and crude protein maintenance in growing Thai-indigenous beef cattle fed rice straw as roughage. J. Anim. Vet. Adv. 9, 2396–2400, https://doi.org/10.3923/javaa.....
7. Connor E.E., Li R.W., Baldwin R.L., Li C., 2010. Gene expression in the digestive tissues of ruminants and their relationships with feeding and digestive processes. Animal 4, 993–1007, https://doi.org/10.1017/S17517....
8. da Silva L.D., Pereira O.G., da Silva T.C., Valadares Filho S.C., Ribeiro K.G., 2016. Effects of silage crop and dietary crude protein levels on digestibility, ruminal fermentation, nitrogen use efficiency, and performance of finishing beef cattle. Anim. Feed Sci. Technol. 220, 22–33, https://doi.org/10.1016/j.anif....
9. Detmann E., Valente E.E.L., Batista E.D., Huhtanen P., 2014. An evaluation of the performance and efficiency of nitrogen utilization in cattle fed tropical grass pastures with supplementation. Livest. Sci. 162, 141–153, https://doi.org/10.1016/j.livs....
10. Ford J.E., Perry K.D., Briggs C.A., 1958. Nutrition of lactic acid bacteria isolated from the rumen. J. Gen. Microbiol. 18, 273–284, https://doi.org/10.1099/002212....
11. Galles K., Ham J., Westover E., Stratton J., Wagner J., Engle T., Bryant T.C., 2011. Influence of reduced nitrogen diets on ammonia emissions from cattle feedlot pens. Atmosphere 2, 655–670, https://doi.org/10.3390/atmos2....
12. Jackowski S., Rock C.O., 1981. Regulation of coenzyme A biosynthesis. J. Bacteriol. 148, 926–932.
13. Li H.Q., Liu Q., Wang C., Guo G., Huo W.J., Zhang S.L., Zhang Y.L., Pei C.X., Yang W.Z., Wang H., 2017. Effects of rumenprotected pantothenate on ruminal fermentation, microbial enzyme activity, cellulolytic bacteria and urinary excretion of purine derivatives in growing beef steers. Livest. Sci 202, 159–165, https://doi.org/10.1016/j.livs....
14. Liu Q., Wang C., Li H.Q., Guo G., Huo W.J., Pei C.X., Zhang S.L., Wang H., 2017. Effects of dietary protein levels and rumenprotected pantothenate on ruminal fermentation, microbial enzyme activity and bacteria population in Blonde d’Aquitaine × Simmental beef steers. Anim. Feed Sci. Technol. 232, 31–39, https://doi.org/10.1016/j.anif....
15. Liu Q., Wang C., Pei C.X. et al., 2014. Effects of isovalerate supplementation on microbial status and rumen enzyme profile in steers fed on corn stover based diet. Livest. Sci. 161, 60–68, https://doi.org/10.1016/j.livs....
16. Liu Q., Wang C., Zhang Y.L., Pei C.X., Zhang S.L., Li H.Q., Guo G., Huo Y.J., Yang W.Z., Wang H., 2016. Effects of 2-methylbutyrate supplementation on growth performance and ruminal development in pre- and post-weaned dairy calves. Anim. Feed Sci. Technol. 216, 129–137, https://doi.org/10.1016/j.anif....
17. Lohakare J.D., Pattanaik A.K., Khan S.A., 2006. Effect of dietary protein levels on the performance, nutrient balances, metabolic profile and thyroid hormones of crossbred calves. Asian-Australas. J. Anim. Sci. 19, 1588–1596, https://doi.org/10.5713/ajas.2....
18. Majee D.N., Schwab E.C., Bertics S.J., Seymour W.M., Shaver R.D., 2003. Lactation performance by dairy cows fed supplemental biotin and a B-vitamin blend. J. Dairy Sci. 86, 2106–2112,https://doi.org/10.3168/jds.S0....
19. Melendez P., Pinedo P., Bastias J., Marin M.P., Rios C., Bustamante C., Adaro N., Duchens M., 2016. The association between serum β-hydroxybutyrate and milk fatty acid profile with special emphasis on conjugated linoleic acid in postpartum Holstein cows. BMC Vet. Res. 12, 50, https://doi.org/10.1186/s12917....
20. National Academies of Sciences, Engineering, and Medicine, 2016. Nutrient Requirements of Beef Cattle. 8th Revised Edition. National Academies Press. Washington, DC (USA), https://doi.org/10.17226/19014.
21. Oh Y.-K., Kim J.-H., Kim K.-H., Choi C.-W., Kang S.-W., Nam I.-S., Kim D.-H., Song M.-K., Kim C.-W., Park K.-K., 2008. Effects of level and degradability of dietary protein on ruminal fermentation and concentrations of soluble non-ammonia nitrogen in ruminal and omasal digesta of Hanwoo steers. Asian-Australas. J. Anim. Sci. 21, 392–403, https://doi.org/10.5713/ajas.2....
22. Ragaller V., Lebzien P., Bigalke W., Südekum K.H., Hüther L., Flachowsky G., 2011. Effects of a pantothenic acid supplementation to different rations on ruminal fermentation, nutrient flow at the duodenum, and on blood and milk variables of dairy cows. J. Anim. Physiol. Anim. Nutr. 95, 730–743,https://doi.org/10.1111/j.1439....
23. Romera J.M., Ramirez M., Gil A., 1996. Determination of pantothenic acid in infant milk formulas by high performance liquid chromatography. J. Dairy Sci. 79, 523–526, https://doi.org/10.3168/jds.S0....
24. Sacadura F.C., Robinson P.H., Evans E., Lordelo M., 2008. Effects of a ruminally protected B-vitamin supplement on milk yield and composition of lactating dairy cows. Anim. Feed Sci. Technol. 144, 111–124, https://doi.org/10.1016/j.anif....
25. Seronde J. Jr., 1963. The pathogenesis of duodenal ulcer disease in the pantothenate-deficient rat. Yale J. Biol. Med. 36, 141–156.
26. Van Soest P.J., Robertson J.B., Lewis B.A., 1991. Methods for dietary fiber, neutral detergent fiber, and non-starch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74, 3583–3597, https://doi.org/10.3168/jds.S0....
27. Völker D., Hüther L., Daş G., Abel H., 2011. Pantothenic acid supplementation to support rumen microbes? Arch. Anim. Nutr. 65, 163–173, https://doi.org/10.1080/174503....
28. Wang C., Liu Q., Guo G., Huo W.J., Ma L., Zhanga Y.L., Pei C.X., Zhang S.L., Wang H., 2016. Effects of rumen-protected folic acid on ruminal fermentation, microbial enzyme activity, cellulolytic bacteria and urinary excretion of purine derivatives in growing beef steers. Anim. Feed Sci. Technol. 221, 185–194, https://doi.org/10.1016/j.anif....
29. Wang C., Liu Q., Li H.Q., Wu X.X., Guo G., Huo W.J., Pei C.X., Zhang Y.L., Zhang S.L., 2018. Effects of rumen-protected pantothenate supplementation on lactation performance, ruminal fermentation, nutrient digestion and blood metabolites in dairy cows. J. Sci. Food Agric. 98, 2098–2104, https://doi.org/10.1002/jsfa.8....
30. Wolin M.J., Miller T.L., Stewart C.S., 1997. Microbe-microbe interactions. In: P.N. Hobson, C.S. Stewart (Editors). The Rumen Microbial Ecosystem. Blackie Academic & Professional (Chapman & Hall). London (UK), pp. 467–491.
31. Zinn R.A., Owens F.N., Stuart R.L., Dunbar J.R., Norman B.B., 1987. B-vitamin supplementation of diets for feedlot calves. J. Anim. Sci. 65, 267–277, https://doi.org/10.2527/jas198....
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