CC-BY 4.0

Effect of an essential oils blend on growth performance, and selected parameters of oxidative stress and antioxidant defence of Escherichia coli challenged piglets

X.R. Jiang 1, 2  ,  
X.L. Li 2,  
A. Awati 3,  
H. Bento 3,  
H.J. Zhang 2,  
V. Bontempo 1  
Università degli Studi di Milano, Department of Health, Animal Science and Food Safety, 20133 Milan, Italy
Chinese Academy of Agricultural Sciences, Feed Research Institute, 100081 Beijing, China
Danisco Animal Nutrition, Dupont Industrial Biosciences, SN8 1XN Marlborough, United Kingdom
J. Anim. Feed Sci. 2017;26(1):38–43
Publish date: 2017-03-21
This study evaluated the effect of diet supplementation with an essential oil blend (13.5% thymol and 4.5% cinnamaldehyde, EO) on growth performance and selected parameters of oxidative stress and antioxidant defence in Escherichia coli challenged piglets. Ninety-six weaned piglets were allocated into 4 experimental treatments (6 replicates per treatment with 4 piglets per replicate) for 35 days arranged in a 2×2 factorial design: diet [a basal diet without additive (CT) or with 50 mg · kg−1 EO] and E. coli challenge [sham (−) or infected (+)]. On day 8, half of the piglets from each dietary group was orally inoculated with 4 ml of E. coli O149:F4 (K88). The E. coli challenge increased the level of malondialdehyde (MDA) in piglet serum on day 9 (P < 0.01), and tended to reduce average daily gain (P = 0.08) and average daily feed intake (P = 0.08) from day 7 to day 35. Dietary EO addition did not affect growth performance but tended to reduce MDA content in piglet serum in comparison with CT group on day 9 (P = 0.07). Neither EO addition nor E. coli challenge influenced antioxidative enzymes (superoxide dismutase and glutathione peroxidase) activity. The obtained results suggest that EO rich in thymol and cinnamaldehyde had tendency to reduce serum lipid peroxidation level by EO addition, however no such effect was observed in piglets challenged by E. coli. So, further studies are needed to confirm the EO influence on systemic antioxidant defence of weaned piglets.
X.R. Jiang   
Chinese Academy of Agricultural Sciences, Feed Research Institute, 100081 Beijing, China
V. Bontempo   
Università degli Studi di Milano, Department of Health, Animal Science and Food Safety, 20133 Milan, Italy
1. Amorati R., Foti M.C., Valgimigli L., 2013. Antioxidant activity of essential oils. J. Agric. Food Chem. 61, 10835–10847, https://doi.org/10.1021/jf403496k
2. Bontempo V., Jiang X.R., Cheli F., Lo Verso L., Mantovani G., Vitari F., Domeneghini C., Agazzi A., 2014. Administration of a novel plant extract product via drinking water to post-weaning piglets: effects on performance and gut health. Animal 8, 721–730, https://doi.org/10.1017/S175173111400041X
3. Bosi P., Casini L., Finamore A., Cremokolini C., Merialdi G., Trevisi P., Nobili F., Mengheri E., 2004. Spray-dried plasma improves growth performance and reduces inflammatory status of weaned pigs challenged with enterotoxigenic Escherichia coli K88. J. Anim. Sci. 82, 1764–1772, https://doi.org/10.2527/2004.8261764x
4. Boudry G., Perrier C., 2008. Thyme and cinnamon extracts induce anion secretion in piglet small intestine via cholinergic pathways. J. Physiol. Pharmacol. 59, 545–552
5. Efe H., Değer O., Kirci D., Karahan S.C., Örem A., Calapoğlu M., 1999. Decreased neutrophil antioxidative enzyme activities and increased lipid peroxidation in hyperlipoproteinemic human subjects. Clin. Chim. Acta 279, 155–165, https://doi.org/10.1016/S0009-8981(98)00178-8
6. Fairbrother J.M., Nadeau E., Gyles C.L., 2005. Escherichia coli in postweaning diarrhea in pigs: an update on bacterial types, pathogenesis, and prevention strategies. Anim. Health Res. Rev. 6, 17–39, https://doi.org/10.1079/AHR2005105
7. Francis D.H., 2002. Enterotoxigenic Escherichia coli infection in pigs and its diagnosis. J. Swine Health Prod. 10, 171–175
8. Han J., Shuvaev V.V., Muzykantov V.R., 2011. Catalase and superoxide dismutase conjugated with platelet endothelial cell adhesion molecule antibody distinctly alleviate abnormal endothelial permeability caused by exogenous reactive oxygen species and vascular endothelial growth factor. J. Pharmacol. Exp. Ther. 338, 82–91, https://doi.org/10.1124/jpet.111.180620
9. Jaeschke H., 1995. Mechanisms of oxidant stress-induced acute tissue injury. Exp. Biol. Med. 209, 104–111, https://doi.org/10.3181/00379727-209-43885b
10. Jiang X.R., Agazzi A., Awati A., Vitari F., Bento H., Ferrari A., Alborali G.L., Crestani M., Domeneghini C., Bontempo V., 2015a. Influence of a blend of essential oils and an enzyme combination on growth performance, microbial counts, ileum microscopic anatomy and the expression of inflammatory mediators in weaned piglets following an Escherichia coli infection. Anim. Feed Sci. Technol. 209, 219–229, https://doi.org/10.1016/j.anifeedsci.2015.08.010
11. Jiang X.R., Awati A., Agazzi A., Vitari F., Ferrari A., Bento H., Crestani M., Domeneghini C., Bontempo V., 2015b. Effects of a blend of essential oils and an enzyme combination on nutrient digestibility, ileum histology and expression of inflammatory mediators in weaned piglets. Animal 9, 417–426, https://doi.org/10.1017/S1751731114002444
12. Jiang X.R., Zhang H.J., Mantovani G., Alborali G.L., Caputo J.M., Savoini G., Dell’Orto V., Bontempo V., 2014. The effect of plant polyphenols on the antioxidant defence system of weaned piglets subjected to an Escherichia coli challenge. J. Anim. Feed Sci. 23, 324–330, https://doi.org/10.22358/jafs/65668/2014
13. Lagouri V., Blekas G., Tsimidou M., Kokkini S., Boskou D., 1993. Composition and antioxidant activity of essential oils from Oregano plants grown wild in Greece. Z. Lebensm. Unters. Forsch. 197, 20–23, https://doi.org/10.1007/BF01202694
14. Liu Y., Song M., Che T.M., Almeida J.A.S., Lee J.J., Bravo D., Maddox C.W., Pettigrew J.E., 2013. Dietary plant extracts alleviate diarrhea and alter immune responses of weaned pigs experimentally infected with a pathogenic Escherichia coli. J. Anim. Sci. 91, 5294–5306, https://doi.org/10.2527/jas.2012-6194
15. Loos M., Geens M., Schauvliege S., Gasthuys F., van der Meulen J., Dubreuil J.D., Goddeeris B.M., Niewold T., Cox E., 2012. Role of heat-stable enterotoxins in the induction of early immune responses in piglets after infection with enterotoxigenic Escherichia coli. PloS ONE 7, e41041, https://doi.org/10.1371/journal.pone.0041041
16. Lykkesfeldt J., Svendsen O., 2007. Oxidants and antioxidants in disease: oxidative stress in farm animals. Vet. J. 173, 502–511, https://doi.org/10.1016/j.tvjl.2006.06.005
17. Maenner K., Vahjen W., Simon O., 2011. Studies on the effects of essential-oil-based feed additives on performance, ileal nutrient digestibility, and selected bacterial groups in the gastrointestinal tract of piglets. J. Anim. Sci. 89, 2106–2112, https://doi.org/10.2527/jas.2010-2950
18. Mancini-Filho J., Van-Koiij A., Mancini D.A., Cozzolino F.F., Torres R.P., 1998. Antioxidant activity of cinnamon (Cinnamomum zeylanicum, Breyne) extracts. Boll. Chim. Farm. 137, 443–447
19. NRC, 2012. Nutrient Requirements of Swine. 11th Revised Edition. National Academy Press. Washington, DC (USA)
20. Si W., Gong J., Chanas C., Cui S., Yu H., Caballero C., Friendship R.M., 2006. In vitro assessment of antimicrobial activity of carvacrol, thymol and cinnamaldehyde towards Salmonella serotype Typhimurium DT104: effects of pig diets and emulsification in hydrocolloids. J. Appl. Microbiol. 100, 1282–1291, https://doi.org/10.1111/j.1365-2672.2006.03045.x
21. Trevisi P., Merialdi G., Mazzoni M., Casini L., Tittarelli C., De Filippi S., Minieri L., Lalatta-Costerbosa G., Bosi P., 2007. Effect of dietary addition of thymol on growth, salivary and gastric function, immune response, and excretion of Salmonella enterica serovar Typhimurium, in weaning pigs challenged with this microbe strain. Ital. J. Anim. Sci. 6, (Suppl. 1), 374-376
22. Ünlü A., Türközkan N., Cimen B., Karabicak U., Yaman H., 2001. The effect of Escherichia coli-derived lipopolysaccharides on plasma levels of malondialdehyde and 3-nitrotyrosine. Clin. Chem. Lab. Med. 39, 491–493, https://doi.org/10.1515/CCLM.2001.081
23. Valko M., Leibfritz D., Moncol J., Cronin M.T.D., Mazur M., Telser J., 2007. Free radicals and antioxidants in normal physiological functions and human disease. Int. J. Biochem. Cell Biol. 39, 44–84, https://doi.org/10.1016/j.biocel.2006.07.001
24. Wang X., Zhao X., 2009. Contribution of oxidative damage to antimicrobial lethality. Antimicrob. Agents Chemother. 53, 1395–1402, https://doi.org/10.1128/AAC.01087-08
25. Windisch W., Schedle K., Plitzner C., Kroismayr A., 2008. Use of phytogenic products as feed additives for swine and poultry. J. Anim. Sci. 86, (Suppl. E), E140–E148, https://doi.org/10.2527/jas.2007-0459
26. Zeng Z., Xu X., Zhang Q., Li P., Zhao P., Li Q., Liu J., Piao X., 2015. Effects of essential oil supplementation of a low-energy diet on performance, intestinal morphology and microflora, immune properties and antioxidant activities in weaned pigs. Anim. Sci. J. 86, 279–285, https://doi.org/10.1111/asj.12277