ORIGINAL PAPER
Effect of FSH stimulation prior to ovum pick-up on follicular
dynamics, oocyte competence, and in vitro embryo production
in Ongole cows (Bos indicus)
1
Sri Venkateswara Veterinary University, Faculty of Veterinary Science, Department of Veterinary Gynaecology and Obstetrics, 517502, Tirupati, India
2
Sri Venkateswara Veterinary University, Faculty of Veterinary Science, LAM Farm, Department of Veterinary Gynaecology and Obstetrics, 522034, Guntur, India
3
Sri Venkateswara Veterinary University, Faculty of Veterinary Science, Department of Veterinary Surgery and Radiology, 517502, Tirupati, India
4
Sri Venkateswara Veterinary University, Department of Veterinary Medicine, 517502, Tirupati, India
Publication date: 2023-06-20
Corresponding author
D. Yasaswini
Sri Venkateswara Veterinary University, Department of Veterinary Medicine, 517502, Tirupati, India
Sri Venkateswara Veterinary University, Department of Veterinary Medicine, 517502, Tirupati, India
J. Anim. Feed Sci. 2023;32(4):354-362
KEYWORDS
TOPICS
ABSTRACT
The current study evaluated the influence of follicle-stimulating
hormone (FSH) administration on the stimulation of ovarian follicles, oocyte
quality, and embryo production rate in Ongole cows with synchronized
follicular wave emergence. Follicular waves were synchronized using 10 μg
of gonadotropin-releasing hormone (GnRH), followed by FSH administration
(250 μg) in three divided doses of 125, 75, and 50 μg at 48, 60, and 72 h post-
GnRH administration, respectively. Although the total number of follicles was
similar in both groups, the hormonal stimulation regimen increased (P < 0.01)
the number of large follicles without affecting small and medium follicles. FSH
stimulation had no effect on the mean count of aspirated follicles; however, the
cows administered FSH showed a higher (P < 0.001) aspiration rate. An analysis
of the proportion of cumulus oocyte complexes revealed that FSH stimulation
did not affect their mean number. Nevertheless, FSH-stimulated cows had
a lower recovery rate (P < 0.001) of cumulus oocyte complexes (COCs) compared
to the control group. In addition, the proportion of good-quality COCs was higher
(P < 0.01), with a simultaneous lower (P < 0.01) percentage of poor-quality
COCs in the FSH group. In vitro fertilization of both good and poor quality
COCs revealed a higher (P < 0.01) blastocyst formation rate within 7 to 8 days
of fertilization. The study concluded that the FSH super-stimulation protocol
improved follicular number, aspiration rate, oocyte competence and blastocyst
percentage in cows synchronized for follicular wave emergence.
ACKNOWLEDGEMENTS
The current research work was performed as
part of the MVSc program of the first author. The
authors are thankful to Sri Venkateswara Veterinary
University (SVVU) for providing facilities for the
research project.
CONFLICT OF INTEREST
The Authors declare that there is no conflict of
interest.
REFERENCES (33)
1.
Baldassarre H., 2021. Laparoscopic Ovum Pick-Up Followed by In Vitro Embryo Production and Transfer in Assisted Breeding Programs for Ruminants. Animals 11, 216, https://doi.org/10.3390/ani110....
2.
Boni R., 2012. Ovum pick-up in cattle: a 25 yr retrospective analysis. Animal Reprod. 9, 362–369.
3.
Bungartz L., Lucas-Hahn A., Rath D., Niemann H., 1995. Collection of oocytes from cattle via follicular aspiration aided by ultrasound with or without gonadotropin pre-treatment and in different reproductive stages. Theriogenology 43, 667–675, https://doi.org/10.1016/0093-6....
4.
Chaubal S.A., Molina J.A., Ohlrichs C.L., Ferre L.B., Faber D.C., Bols P.E.J., Riesen J.W., Tian X., Yang X., 2006. Comparison of different transvaginal ovum pick-up protocols to optimize oocyte retrieval and embryo production over a 10-week period in cows. Theriogenology 65, 1631–1648, https://doi.org/10.1016/j.ther....
5.
Cox E., Takov V., 2022. Embryology, Ovarian Follicle Development. In: Stat Pearls. Treasure Island (FL): StatPearls Publishing.
6.
da Silva J.C.B., Ferreira R.M., Filho M.M., Naves J.R., Santin T., Pugliesi G., Madureira E.H., 2017. Use of FSH in two different regimens for ovarian super stimulation prior to ovum pick up and in vitro embryo production in Holstein cows. Theriogenology 90,65–73, https://doi.org/10.1016/j.ther....
7.
De Roover R., Feugang J.M.N., Bols P.E.J., Genicot G., Hanzen Ch., 2008. Effects of ovum pick-up frequency and FSH stimulation: a retrospective study on seven years of beef cattle in vitro embryo production. Reprod. Dom. Anim. 43, 239–245, https://doi.org/10.1111/j.1439....
8.
De Souza D., Salles L., Camargo R., Gulart L., Costa e Silva S., De Lima B., Torres F.A.G., Rosa e Silva A., 2018. Effects of PI3K and FSH on steroidogenesis, viability and embryo development of the cumulus–oocyte complex after in vitro culture. Zygote, 26, 50–61, https://doi.org/10.1017/S09671....
9.
Dorice A.K., Ferdinand N., Justin K., Augustave K., Linda K.K., 2019. Effects of breed, age, body condition score, and nutritional status on follicular population, oocyte yield, and quality in three Cameroonian Zebus cattle Bos indicus. Adv. Agri., id: 2979740, https://doi.org/10.1155/2019/2....
10.
Ferre L.B., Kjelland M.E., Strøbech L.B., Hyttel P., Mermillod P., Ross P.J., 2020. Review: Recent advances in bovine in vitro embryo production: reproductive biotechnology history and methods. Animal 14, 991–1004, https://doi.org/10.1017/S17517....
11.
Ginther O.J., Kastelic J.P., Knopf L., 1989. Composition and characteristics of follicular waves during the bovine estrous cycle. Anim. Reprod. Sci. 20, 187–200, https://doi.org/10.1016/0378-4....
12.
Goodhand K.L., Staines M.E., Hutchinson J.S.M., Broadbent P.J., 2000. In-vivo oocyte recovery and in-vitro embryo production from bovine oocyte donors treated with progestogen, estradiol and FSH. Anim. Reprod. Sci. 63, 145–158, https://doi.org/10.1016/s0378-....
13.
Hayden C.B., Sala R.V., Absalón-Medina V.A., Motta J.C.L., Pereira D., Moreno J.F., García-Guerra A., 2022. Synchronization of follicle wave emergence before ovarian superstimulation with FSH and ovum pick-up improves in vitro embryo production in pregnant heifers. Theriogenology 188, 71–78, https://doi.org/10.1016/j.ther....
14.
Helmer A., Magaton I., Stalder O., Stute P., Surbek D., von Wolff M., 2022. Optimal timing of ovulation triggering to achieve highest success rates in natural cycles - an analysis based on follicle size and oestradiol concentration in natural cycle IVF. Front. Endocrinol. 13, 855131, https://doi.org/10.3389/fendo.....
15.
Hendriksen P.J., Steenweg W.N.M., Harkema J.C., Merton J.S., Bevers M.M., Vos P.L.A.M., Dieleman S.J., 2004. Effect of different stages of the follicular wave on in vitro developmental competence of bovine oocytes. Theriogenology 61, 909–920, https://doi.org/10.1016/S0093-....
16.
Iwata H., 2017. Age-associated changes in granulosa cells and follicular fluid in cows. J. Reprod. Dev. 63, 339–345, https://doi.org/10.1262/jrd.20....
17.
Jeyakumar S., 2004. Ultrasound guided transvaginal follicular aspiration and in-vitro embryo production in cows. Ph.D. thesis.
18.
Kahraman S., Çetinkaya C.P., Çetinkaya M., Tüfekçi M.A., Ekmekçi C.G., Montag M., 2018. Is there a correlation between follicle size and gene expression in cumulus cells and is gene expression an indicator of embryo development? Reprod. Biol. Endocrinol. 16, 69, https://doi.org/10.1186/s12958....
19.
Livestock Census, 2019. 20th Livestock Census of India-Key Results (Provisional). Ministry of Fisheries, Animal Husbandry and Dairying. Department of Animal Husbandry and Dairying, Krishi Bhavan, New Delhi. 2019, https://pib.gov.in/PressReleas....
20.
Looney C.R., Lindsey B.R., Gonseth C.L., Johnson D.L., 1994. Commercial aspects of oocyte retrieval and in vitro fertilization (IVF) for embryo production in problem cows. Theriogenology 41, 67–72, https://doi.org/10.1016/S0093-....
21.
Majeed A.F., Omar A.A., Alrawi S.T.J., Al-Maadhidy A.H., Ahmed K.D., Khudair M., 2019. Effect of collection methods on oocyte recovery rate in sheep. Res. J. Biotechnol, 14, 262–264.
22.
Manik R.S., Singla S.K., Palta P., 2003. Collection of oocytes through transvaginal ultrasound-guided aspiration of follicles in an Indian breed of cattle. Anim. Reprod. Sci. 76, 155–161, https://doi.org/10.1016/S0378-....
23.
Nagai K., Yanagawa Y., Katagiri S., Nagano M., 2015. Fertilizability of oocytes derived from Holstein cows having different antral follicle counts in ovaries. Anim. Reprod. Sci. 163, 172–178, https://doi.org/10.1016/j.anir....
24.
PETA (People for the Ethical Treatment of Animals)., 1960. Prevention of Cruelty to Animals Act - Overview of Animal Laws in India | Animal Legal & Historical Center. www.animallaw.info. Retrieved 2 December 2022.
25.
Petrovas G., Kosior M.A., Presicce G.A., Russo M., Zullo G., Albero G., Alkan S., Gasparrini B., 2020. FSH stimulation with short withdrawal improves oocyte competence in italian mediterranean buffalo (Bubalus bubalis). Animals 10, 1997, https://doi.org/10.3390/ani101....
26.
Pieterse M.C., Vos P.L.A.M., Kruip Th.A.M., Wurth Y.A., van Beneden Th. H., Willemse A.H., Taverne M.A.M., 1991. Transvaginal ultrasound guided follicular aspiration of bovine oocytes. Theriogenology 35, 857–862, https://doi.org/10.1016/0093-6....
27.
Prevention of Cruelty to Animals Act, 1960 – An Overview. 1960. Corner, Law (25 June 2021), https://lawcorner.in/preventio... (Retrieved on 2 December 2022).
28.
Reddy P.R., Hiremath S., Sudhakar K., Metta M., Vinoo R., Reddy P.R. 2021. Genetic analysis of production and reproduction traits of Ongole cattle in an organized farm of Andhra Pradesh, https://doi.org/10.18805/IJAR.....
29.
Saini N., Singh M.K., Shah S.M., Singh K.P., Kaushik R., Manik R.S., Singla S.K., Palta P., Chauhan M.S., 2015. Developmental competence of different quality bovine oocytes retrieved through ovum pick-up following in vitro maturation and fertilization. Animal 9, 1979–1985, https://doi.org/10.1017/S17517....
30.
Seneda M.M., Esper C.R., Garcia J.M., Oliveira J.A., Vantini R., 2001. Relationship between follicle size and ultrasound-guided transvaginal oocyte recovery. Anim. Reprod. Sci. 67, 37–43, https://doi.org/10.1016/S0378-....
31.
Van Eenennaam A.L., 2019. Application of genome editing in farm animals: cattle. Transgenic Res. 28, 93–100, https://doi.org/10.1007/s11248....
32.
Vennapureddy S., Arunakumari G., Reddy K.C., Reddy K.R., Ambica, G., 2022. Effect of FSH stimulation of ovaries on in vitro maturation of sahiwal oocytes collected by ovum pick-up (OPU) method. Ind. J. Anim. Res. https://doi.org/10.18805/IJAR.....
33.
Walton J.S., Christie K.A., Stubbings R.B., 1993. Evaluation of frequency of ultrasonically guided fo llicle aspiration on bovine ovarian dynamics. Theriogenology 39, 336, https://doi.org/10.1016/0093-6....
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