The objective of the experiment was to fnd a quantitative test to determine the lysine or methionine bioavailability of rumen-protected amino acids. For these purposes we elaborated a blood
test including an in vivo calibration phase and we used this test on two commercial products whose
bioavailability is known (SmartamineM™ and SmartamineML™). Three ruminally and duodenally
fistulated low-yielding (10 kg/d) Holstein cows were used. The calibration phase consisted of relating blood plasma methionine and lysine concentrations to duodenal infusion of graded amounts of
methionine and lysine. The blood plasma responses of methionine and lysine to graded amounts
infused duodenally were linear. The methionine response was the same for all cows, but the lysine
response varied with the cows. Quantification of the bioavailability of commercial products is achieved
by relating the blood level variations, as induced by product supplementation, to those obtained by
duodenal infusion during the calibration phase. Bioavailability of methionine supplied by SmartamineM™ and SmartamineML™ was 75 and 84%, respectively. The reliability of the test is related
to the amount of amino acid provided by the product supplementation: providing a low quantity of
amino acids resulted in an overestimation of the bioavailability. This tendency is particularly clear
for lysine bioavailability estimation. The proposed blood test is valuable for the determination of the
bioavailability of methionine, but not lysine, of rumen-protected products.
Towards non-invasive methods to determine the effect of treatment of soya-bean meal on lysine availability in dairy cows S. I. Borucki Castro, H. Lapierre, L. E. Phillip, P. W. Jardon, R. Berthiaume animal
Effects of ruminally protected methionine and/or phenylalanine on performance of high producing Holstein cows fed rations with very high levels of canola meal N. Swanepoel, P.H. Robinson, L.J. Erasmus Animal Feed Science and Technology
Hydroxymethyl lysine is a source of bioavailable lysine for ruminants1 E. A. Elwakeel, E. C. Titgemeyer, B. R. Faris, D. W. Brake, A. M. Nour, M. E. A. Nasser Journal of Animal Science
Effects of providing two forms of supplemental methionine to periparturient Holstein dairy cows on feed intake and lactational performance R.S. Ordway, S.E. Boucher, N.L. Whitehouse, C.G. Schwab, B.K. Sloan Journal of Dairy Science
Effect of Different Forms of Methionine on Lactational Performance of Dairy Cows H. Rulquin, B. Graulet, L. Delaby, J.C. Robert Journal of Dairy Science
Methionine Availability in Plasma of Dairy Cows Supplemented with Methionine Hydroxy Analog Isopropyl Ester B. Graulet, C. Richard, J.C. Robert Journal of Dairy Science
Lysine bioavailability among 2 lipid-coated lysine products after exposure to silage1 J. Reiners, J. Held, C. Wright, Q. Qiao, G. Djira, B. Brunsvig, K. Reza, D. Brake Translational Animal Science
Bioavailability of rumen-protected methionine, lysine and histidine assessed by fecal amino acid excretion S.E. Räisänen, C.M.M.R. Martins, K. Nedelkov, J. Oh, M.T. Harper, A. Melgar, X. Chen, C. Parys, R.A. Patton, M. Miura, A.N. Hristov Animal Feed Science and Technology
Relative availability of metabolizable methionine from 2 ruminally protected sources of methionine fed to lactating dairy cattle M. Ardalan, C.F. Vargas-Rodriguez, G.I. Zanton, M. Vázquez-Añón, B.J. Bradford, E.C. Titgemeyer Journal of Dairy Science
Effects of rumen-protected methionine supplementation on the performance of high production dairy cows in the tropics Valdir Junior, Fernanda Lopes, Charles Schwab, Mateus Toledo, Edgar Collao-Saenz
Effects of polymerization of casein and sources of lysine on amino acid bioavailability among calves fed liquid-based diets E.A. Petzel, S. Acharya, E.A. Bailey, D.W. Brake Journal of Dairy Science
Relative bioavailability of 3 rumen-undegradable methionine sources in dairy cows using the area under the curve technique A. Kihal, M. Rodriguez-Prado, S. Calsamiglia JDS Communications
Effects of rumen-protected methionine supplementation on the performance of high production dairy cows in the tropics Valdir Junior, Fernanda Lopes, Charles Schwab, Mateus Toledo, Edgar Collao-Saenz, Juan Loor PLOS ONE
In situ and in vitro evaluation of the bioavailability of rumen-protected methionine with coating prototypes Yu Zhang, Chenxue Zhang, Mengmeng Zhang, Huan Yang, Fangfang Zhao, Ning Jiang, Aizhong Zhang Journal of the Mechanical Behavior of Biomedical Materials
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