Pesticide residues in milk and their relationship with pesticide contamination of feedstuffs supplied to dairy cattle in Punjab (India)
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Guru Angad Dev Veterinary and Animal Sciences University, School of Public Health and Zoonoses, Ludhiana, Punnjab-141001, India
J. S. Bedi   

Guru Angad Dev Veterinary and Animal Sciences University, School of Public Health and Zoonoses, Ludhiana, Punnjab-141001, India
Publication date: 2018-01-23
J. Anim. Feed Sci. 2018;27(1):18–25
The aim of the present study was to investigate the correlation between pesticides contamination of feedstuffs supplied to dairy cattle and pesticide residues in bovine milk. The animal concentrate feed, fodder, water and milk samples were collected from 55 dairy farms in Punjab (India) representing intensive production and typical feeding management. The concentrate feed, fodder/silage, water and milk samples were extracted by multiple residue methodology and pesticide residues concentration was estimated by gas chromatography. It was shown that chlorpyrifos pesticide was the main contaminant detected at mean level of 6.01, 4.05 and 2.58 ng · g−1 in concentrate feed, fodder and milk samples, respectively. Other pesticide residues observed in feed and milk samples were: endosulphan sulphate, cypermethrin, ethion, p,p’-DDE, lindane, malathion and fenvalerate. Chlorpyrifos was the only pesticide detected in one water sample. Though the mean residue levels of pesticides were substantially below the maximum residue limits (MRL), individually two milk samples exceeded the MRL value for lindane (1.0 ng · g−1) and three for DDT (20.0 ng · g−1) and chlorpyrifos (20.0 ng · g−1). Two milk samples also violated the MRL value (10.0 ng · g−1) for endosulphan sulphate residues. However, none of the samples was found above the MRL (50.0 ng · g−1) for cypermethrin. The relationship between occurrence of pesticide residues in feedstuff and milk might suggest that animal feed is the main source for appearance of pesticides in milk. However, environmental contamination with pesticides, lack of good management practices while using pesticides and violation of withdrawal period for ectoparasiticides may also result in the occurrence of pesticide residues in milk.
Battu R.S., Singh B., Kang B.K., 2004. Contamination of liquid milk and butter with pesticide residues in the Ludhiana district of Punjab state, India. Ecotox. Environ. Safe. 59, 324–331, https://doi.org/10.1016/j.ecoe....
Bedi J.S., Gill J.P.S., Aulakh R.S., Kaur P., Sharma A., Pooni P.A., 2013. Pesticide residues in human breast milk: Risk assessment for infants in the Punjab, India. Sci. Total Environ. 463–464, 720–726, https://doi.org/10.1016/j.scit....
Bedi J.S., Gill J.P.S., Aulakh R.S., Kaur P., 2015. Pesticide residues in bovine milk in Punjab, India: Spatial variation and risk assessment to human health. Arch. Environ. Contam. Toxicol. 69, 230–240, https://doi.org/10.1007/s00244....
Cheema H.K., Kang B.K., Singh B., 2004. Monitoring of HCH residues in bovine milk in Punjab, India. Indian J. Environ. Toxicol. 14, 54–58.
Chakraborty P., Zhang G., Li J., Xu Y., Liu X., Tanabe S., Jones K.C., 2010. Selected organochlorine pesticides in the atmosphere of major Indian cities: levels, regional versus local variations, and sources. Environ. Sci. Technol. 44, 8038–8043, https://doi.org/10.1021/es1020....
Devi N.L., Qi S., Chakraborty P., Zhang G., Yadav I.C., 2011. Passive air sampling of organochlorine pesticides in a northeastern state of India, Manipur. J. Environ. Sci. 23, 808–815, https://doi.org/10.1016/S1001-....
Fajgelj A., Ambrus A., 2000. Guidelines for single-laboratory validation of analytical methods for trace level concentrations of organic chemicals. In: A. Fajgelj., A. Ambrus (Editors). Principles and Practices of Method Validation. Royal Society of Chemistry. Cambridge (UK), https://doi.org/10.1039/978184....
FAO/WHO, 2016. Codex Pesticides Residues in Food Online Database. Codex Alimentarius Commission, FAO and WHO. Rome (Italy), available at: http://www.fao.org/fao-who-cod....
Feo M.L., Eljarrat E., Manaca M.N., Dobaño C., Barcelo D., Sunyer J., Alonso P.L., Menendez C., Grimalt J.O., 2012. Pyrethroid use-malaria control and individual applications by households for other pests and home garden use. Environ. Int. 38, 67–72, https://doi.org/10.1016/j.envi....
FSSAI (Food Safety and Standard Authority of India), 2011. Food Safety and Standards (Contaminants, Toxins And Residues) Regulations, 2011, available at: http://fssai.gov.in/dam/jcr:75....
García de Llasera M.P., Bernal-González M., 2001. Presence of carbamate pesticides in environmental waters from the northwest of Mexico: determination by liquid chromatography. Water Res. 35, 1933–1940, https://doi.org/10.1016/S0043-....
Goulart S.M., de Queiroz M.E.L.R., Neves A.A., de Queiroz J.H., 2008. Low-temperature clean-up method for the determination of pyrethroids in milk using gas chromatography with electron capture detection. Talanta 75, 1320–1323, https://doi.org/10.1016/j.tala....
Government of India, Ministry of Agriculture & Farmers Welfare, 2015. Statistical Database: Production of Key Pesticides during 2010-11 to 2015-16. Available at: http://ppqs.gov.in/statistical....
Hernandez F., Beltran J., Sancho J.V., 1993. Study of multi-residue methods for the determination of selected pesticides in groundwater. Sci. Total. Environ. 132, 297–312, https://doi.org/10.1016/0048-9....
International Dairy Federation (IDF), 1997. Residues and Contaminants in Milk and Milk Products. Ref. S.I. 9701. IDF. Brussels (Belgium).
Kan C.A., Meijer G.A.L., 2007. The risk of contamination of food with toxic substances present in animal feed. Anim. Feed. Sci. Technol. 133, 84–108, https://doi.org/10.1016/j.anif....
Kang B.K., Singh B., Chahal K.K., Battu R.S., 2002. Contamination of feed concentrate and green fodder with pesticide residues. Pest. Res. J. 14, 308–312.
Mills P.A., Bong B.A., Kamps L.R., Burke J.A., 1972. Elution solvent system for Florisil column cleanup in organochlorine pesticide residue analyses. J. Assoc. Off. Anal. Chem. 55, 39–43.
Nag S.K., Raikwar M.K., 2011. Persistent organochlorine pesticide residues in animal feed. Environ. Monit. Assess. 174, 327–335, https://doi.org/10.1007/s10661....
Pagliuca G., Serraino A., Gazzotti T., Zironi E., Borsari A., Rosmini R., 2006. Organophosphorus pesticides residues in Italian raw milk. J. Dairy. Res. 73, 340–344, https://doi.org/10.1017/S00220....
Rothwell J.T., Burnett T.J., Hacket K., Chevis R., Lowe L.B., 2001. Residues of zeta-cypermethrin in bovine tissues and milk following pour-on and spray application. Pest Manag. Sci. 57, 993–999, https://doi.org/10.1002/ps.372.
Sankararamakrishnan N., Sharma A.K., Sanghi R., 2005. Organochlorine and organophosphorous pesticide residues in ground water and surface waters of Kanpur, Uttar Pradesh, India. Environ. Int. 31, 113–120, https://doi.org/10.1016/j.envi....
Sharma V., Wadhwa B.K., Stan H.J., 2005. Multiresidue analysis of pesticides in animal feed concentrate. Bull. Environ. Contam. Toxicol. 74, 342–349, https://doi.org/10.1007/s00128....
Sharma H.R., Kaushik A., Kaushik C.P., 2007. Pesticide residues in bovine milk from a predominantly agricultural state of Haryan, India. Environ. Monit. Assess. 129, 349–357, https://doi.org/10.1007/s10661....
Singh N.K., Jyoti, Haque M., Singh H., Rath S.S., Ghosh S., 2014. A comparative study on cypermethrin resistance in Rhipicephalus (Boophilus) microplus and Hyalomma anatolicum from Punjab (India). Ticks Tick-Borne Dis. 5, 90–94, https://doi.org/10.1016/j.ttbd....
Srimurali S., Govindaraj S., Kumar S.K., Rajendran R.B., 2015. Distribution of organochlorine pesticides in atmospheric air of Tamilnadu, southern India. Int. J. Environ. Sci. Technol. 12, 1957–1964, https://doi.org/10.1007/s13762....
Tsiplakou E., Anagnostopoulos C.J., Liapis K., Haroutounian S.A., Zervas G., 2010. Pesticides residues in milks and feedstuff of farm animals drawn from Greece. Chemosphere 80, 504–512, https://doi.org/10.1016/j.chem....
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