0.857
IF5
0.900
IF
Q3
JCR
0.92
CiteScore
0.405
SJR
Q2
SJR
20
MNiSW
142.18
ICV
SHORT COMMUNICATION
 
CC-BY 4.0
 
 

Effects of methionine and its ratio to lysine on expression of αs1 casein gene in cultured bovine mammary epithelial cells

H. Y. Liu 1,  
J. Y. Yang 1,  
H. H. Wu 1,  
Y. M. Wu 1  ,  
J. X. Liu 1  
 
1
Institute of Dairy Science, Ministry of Education Key Laboratory of Molecular Animal Nutrition, Zhejiang University, Hangzhou 310029, P. R. China
J. Anim. Feed Sci. 2007;16(Suppl. 2):330–334
Publish date: 2007-09-17
KEYWORDS:
ABSTRACT:
Effects of methionine dose and its ratio to lysine on αs1 casein gene expression were evaluated in cultured bovine mammary epithelial cells. Methionine was added at levels of 0-100 μg/ml and ratio of lysine to methionine was set at 1-4.5:1. Expression of αs1 casein gene was enhanced by addition of methionine up to 60 μg/ml, and then leveled off. There was an increasing trend in αs1 casein expression with the increasing ratio of lysine to methionine and the highest level was at 3.5:1. These results indicated that adequate quantities and ratio of amino acids are required to promote milk protein synthesis.
CORRESPONDING AUTHOR:
Y. M. Wu   
Institute of Dairy Science, Ministry of Education Key Laboratory of Molecular Animal Nutrition, Zhejiang University, Hangzhou 310029, P. R. China
J. X. Liu   
Institute of Dairy Science, Ministry of Education Key Laboratory of Molecular Animal Nutrition, Zhejiang University, Hangzhou 310029, P. R. China
 
CITATIONS (7):
1. Effects of dietary supplementation of methionine and lysine on milk production and nitrogen utilization in dairy cows1
C. Wang, H.Y. Liu, Y.M. Wang, Z.Q. Yang, J.X. Liu, Y.M. Wu, T. Yan, H.W. Ye
Journal of Dairy Science
2. Establishment and characterization of a lactating bovine mammary epithelial cell model for the study of milk synthesis
Ke Zhao, Hong‑Yun Liu, Miao‑Miao Zhou, Jian‑Xin Liu
Cell Biology International
3. Effects of phenylalanine and threonine oligopeptides on milk protein synthesis in cultured bovine mammary epithelial cells
M. M. Zhou, Y. M. Wu, H. Y. Liu, J. X. Liu
Journal of Animal Physiology and Animal Nutrition
4. Methionine protects against hyperthermia-induced cell injury in cultured bovine mammary epithelial cells
Zhao-Yu Han, Tian Mu, Zhen Yang
Cell Stress and Chaperones
5. Impact of Heat Stress on Cellular and Transcriptional Adaptation of Mammary Epithelial Cells in Riverine Buffalo (Bubalus Bubalis)
Neha Kapila, Ankita Sharma, Amit Kishore, Monika Sodhi, Pawan K. Tripathi, Ashok K. Mohanty, Manishi Mukesh, Yiqun G. Shellman
PLOS ONE
6. Effect of dietary N-carbamoylglutamate on milk production and nitrogen utilization in high-yielding dairy cows
B. Chacher, W. Zhu, J.A. Ye, D.M. Wang, J.X. Liu
Journal of Dairy Science
7. Lysine Stimulates Protein Synthesis by Promoting the Expression of ATB0,+ and Activating the mTOR Pathway in Bovine Mammary Epithelial Cells
Xiujuan Lin, Shanshan Li, Yixuan Zou, Feng-Qi Zhao, Jianxin Liu, Hongyun Liu
The Journal of Nutrition
ISSN:1230-1388