REVIEW PAPER
Comparison of two buffers on in vitro gas production, pH and NH3-N concentration of feedstuffs for ruminants
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State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100094, P.R. China
CORRESPONDING AUTHOR
G.-Y. Zhao   

State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100094, P.R. China
Publication date: 2007-09-17
 
J. Anim. Feed Sci. 2007;16(Suppl. 2):512–516
 
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ABSTRACT
The objective of the study was to compare Menke et al.’s buffer (1979) with Zhao and Lebzien’s buffer (2000) on gas production, pH and NH3-N of 16 feedstuffs for ruminants in in vitro incubation. The gas production was recorded after incubation at 12 and 24 h. The pH and NH3-N concentration of incubation residues were determined after incubation for 24 h. The results showed that there was no significant difference in in vitro gas production, pH and NH3-N concentration between the two buffers (P>0.05). It was found that in vitro gas production, pH or NH3-N concentration of the two buffers were significantly correlated (P<0.05). It was concluded that the two buffers had similar results on in vitro gas production, pH and NH3-N concentration in in vitro incubation. It is suggested to compare these two buffers on volatile fatty acids (VFA) concentration and rumen microorganism activities in the future.
ISSN:1230-1388