ORIGINAL PAPER
Comparison of high-performance ion chromatography technique with microbiological assay of myo-inositol in plant components
of poultry feeds
1 |
University of Agriculture in Krakow, Faculty of Food Technology,
Department of Food Biotechnology,
Balicka 122, 30-149 Kraków, Poland |
J. Anim. Feed Sci. 2011;20(1):143–156
Publication date: 2011-03-12
KEYWORDS
ABSTRACT
An ion chromatography technique (HPAEC-PAD) was employed for determination of inositol in plant components of feeds as an alternative to a microbiological assay. The study included analysis of total, free and dialysable inositol released from samples by an in vitro procedure simulating digestion in the gastrointestinal tract of birds. Inositol was separated on the high-performance anion-exchange CarboPack MA1 column and monitored by the pulsed amperometric detection (PAD). As compared to the HPAEC-PAD technique, microbiological assay produced 2-3 fold lower values of total inositol, probably due to myo-inositol stereoselectivity of Saccharomyces cerevisiae ATCC 9080 analytical strain. The overall correlation coefficient was 0.989, 0.941 and 0.736 for total, free and dialysable inositol, respectively. Microbiological method of analysis rather than the HPAEC-PAD technique may be recommended for determination of dialysable myo-inositol released from feed samples during in vitro digestions that simulate gastrointestinal tract of birds. The HPAEC-PAD technique was more sensitive, convenient and suitable for total inositol determination.
CORRESPONDING AUTHOR
K. Żyła
University of Agriculture in Krakow, Faculty of Food Technology,
Department of Food Biotechnology,
Balicka 122, 30-149 Kraków, Poland
CITATIONS (10):
1. |
Myo-inositol phosphates profile of buckwheat and quinoa seeds: Effects of hydrothermal processing and solid-state fermentation with Rhizopus oligosporus Robert Duliński, Anna Starzyńska-Janiszewska, Łukasz Byczyński, Urszula Błaszczyk International Journal of Food Properties
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2. |
Profile and bioavailability analysis ofmyo-inositol phosphates in rye bread supplemented with phytases: a study using anin vitromethod and Caco-2 monolayers R. Duliński, E. K. Cielecka, M. Pierzchalska, Ł. Byczyński, K. Żyła International Journal of Food Sciences and Nutrition
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3. |
A window on cyclitols: Characterization and analytics of inositols Hossam Al-Suod, Magdalena Ligor, Ileana-Andreea Rațiu, Katarzyna Rafińska, Ryszard Górecki, Bogusław Buszewski Phytochemistry Letters
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4. |
Fermentation of Colored Quinoa Seeds with Neurospora intermedia to Obtain Oncom-Type Products of Favorable Nutritional and Bioactive Characteristics Anna Starzyńska-Janiszewska, Bożena Stodolak, Robert Duliński, Barbara Mickowska, Renata Sabat Cereal Chemistry Journal
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5. |
The free and total myo-inositol contents of early lactation and seasonal bovine milk Harvey E. Indyk, Sheila C. Saldo, Peter M. White, Mumtaz N. Dole, Brendon D. Gill, David C. Woollard International Dairy Journal
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Inositol - An effective growth promotor? S.A. LEE, M.R. BEDFORD World's Poultry Science Journal
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7. |
Impact of Two Commercial Enzymes on the Release of Inositols, Fermentable Sugars, and Peptides in the Technology of Buckwheat Beer Robert Duliński, Marek Zdaniewicz, Aneta Pater, Krzysztof Żyła Journal of the American Society of Brewing Chemists
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8. |
Effects of inositol, inositol-generating phytase B applied alone, and in combination with 6-phytase A to phosphorus-deficient diets on laying performance, eggshell quality, yolk cholesterol, and fatty acid deposition in laying hens K. Żyła, M. Mika, R. Duliński, S. Świątkiewicz, J. Koreleski, H. Pustkowiak, J. Piironen Poultry Science
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9. |
Effect of inositol and phytases on hematological indices and α-1 acid glycoprotein levels in laying hens fed phosphorus-deficient corn-soybean meal-based diets K. Żyła, M. Grabacka, M. Pierzchalska, R. Duliński, A. Starzyńska-Janiszewska Poultry Science
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10. |
The Impact of Phytases on the Release of Bioactive Inositols, the Profile of Inositol Phosphates, and the Release of Selected Minerals in the Technology of Buckwheat Beer Production Robert Duliński, Marek Zdaniewicz, Aneta Pater, Dagmara Poniewska, Krzysztof Żyła Biomolecules
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