In-ovo and dietary supplementation of selenium nano-particles influence physiological responses, immunological status and performance of broiler chicks
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Egyptian Atomic Energy Authority, Nuclear Research Center, Biological Application Department, Abou-Zabael 13759, Egypt
Ain Shams University, Faculty of Agriculture, Poultry Production Department, Cairo, 11241, Egypt
N.S. Ibrahim   

Egyptian Atomic Energy Authority, Nuclear Research Center, Biological Application Department, Abou-Zabael 13759, Egypt
Publication date: 2020-03-31
J. Anim. Feed Sci. 2020;29(1):46–58
This study aimed to explore impacts of in ovo injection and dietary supplementation of selenium nanoparticles (SeNPs) on physiological responses, immunological status and performance of broiler chicks. A total of 210 broiler eggs were divided into 3 in ovo groups: not in ovo injected and in ovo injected with 5 or 10 ppb SeNPs. The hatched chicks from each in ovo group were divided into 2 dietary groups, with or without 10 ppb SeNPs/kg ration for 5 weeks. Live body weight and body weight gain were significantly increased in all SeNPs injected or supplemented groups. Feed conversion ratio for the whole feeding period was improved by SeNPs feed supplementation. Serum triglycerides and malondialdehyde contents were significantly decreased, while high-density lipoprotein cholesterol, immunoglobulin status, reduced glutathione content and glutathione reductase activity were increased by both examined routes of SeNPs delivery. It can be concluded that SeNPs in ovo injection with a dose up to 10 ppb/egg along with SeNPs diet supplementation at a dose of 10 ppb/kg had a positive effect on performance and physiological, antioxidant and immunological status of broiler chicks.
Allan W.H., Gough R.E., 1974. A standard haemagglutination inhibition test for Newcastle disease. (1). A comparison of macro and micro methods. Vet. Rec. 95, 120–123, https://doi.org/10.1136/vr.95.....
Bhanja S.K., Mandal A.B., Johri T.S., 2004. Standardization of injection site, needle length, embryonic age and concentration of amino acids for in ovo injection in broiler breeder eggs. Indian J. Poult. Sci. 39, 105–111.
Biswas A., Mohan J., Sastry K.V.H., 2006. Effect of higher levels of dietary selenium on production performance and immune responses in growing Japanese quail. Br. Poult. Sci. 47, 511–515, https://doi.org/10.1080/000716....
Boostani A., Sadeghi A.A., Mousavi S.N., Chamani M., Kashan N., 2015. The effects of organic, inorganic, and nano-selenium on blood attributes in broiler chickens exposed to oxidative stress. Acta Sci. Vet. 43, 1264.
Cai S.J., Wu C.X., Gong L.M., Song T., Wu H., Zhang L.Y., 2012. Effects of nano-selenium on performance, meat quality, immune function, oxidation resistance, and tissue selenium content in broilers. Poult. Sci. 91, 2532–2539, https://doi.org/10.3382/ps.201....
Cantor A.H., Moorhead P.D., Musser M.A., 1982. Comparative effects of sodium selenite and selenomethionine upon nutritional muscular dystrophy, selenium-dependent glutathione peroxidase, and tissue selenium concentrations of turkey poults. Poult. Sci. 61, 478–484, https://doi.org/10.3382/ps.061....
Chen G., Wu J., Li C., 2013.The effect of different selenium levels on production performance and biochemical parameters of broilers. Ital. J. Anim. Sci. 12, e79, https://doi.org/10.4081/ijas.2....
Choupani M., Moghadam P.Z., Kelidari H.R., Ghaz S., 2014. Influence of dietary selenium sources on thyroid hormone activation, tissue selenium distribution and antioxidant enzymes status in broiler chickens. Trends Life Sci. 3, 281–297.
Duncan D.B., 1955. Multiple rang and multiple F-tests. Biometrics 11, 1–42, https://doi.org/10.2307/300147....
Ebeid T.A., Zeweil H.S., Basyony M.M., Dosoky W.M., Badry H., 2013. Fortification of rabbit diets with vitamin E or selenium affects growth performance, lipid peroxidation, oxidative status and immune response in growing rabbits. Livest. Sci. 155, 323–331, https://doi.org/10.1016/j.livs....
Elsaid A.E., 2015. Influence of in-ovo injection with nanoparticles selenium (SeNPs) on growth performance, carcass yield and immune status of broiler chicks PhD Thesis. Damietta University, Faculty of Agriculture. Damietta (Egypt).
Heindl J., Ledvinka Z., Englmaierová M., Zita L., Tůmová E., 2010. The effect of dietary selenium sources and levels on performance, selenium content in muscle and glutathione peroxidase activity in broiler chickens. Czech J. Anim. Sci. 55, 572–578, https://doi.org/10.17221/2487-....
Jamil Z., 2013. Effects of inorganic and nano form of selenium on growth performance and biochemical indices of mahseer (Tor Putitora). MPhil. Thesis. Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad (Pakistan).
Jiang Z., Lin Y., Zhou G., Luo L., Jiang S., Chen F., 2009. Effects of dietary selenomethionine supplementation on growth performance, meat quality and antioxidant property in yellow broilers. J. Agric. Food Chem. 57, 9769–9772, https://doi.org/10.1021/jf9024....
Jianhua H., Ohtsuka A., Hayashi K., 2000. Selenium influences growth via thyroid hormone status in broiler chickens. Br. J. Nutr. 84, 727–732, https://doi.org/10.1017/S00071....
Keen C.L., Uriu-Adams J.Y., Ensunsa J.L., Gershwin M.E., 2004. Trace elements/minerals and immunity. In: M.E. Gershwin, P. Nestel, C.L. Keen (Editors). Handbook of Nutrition and Immunity. Humana Press. Totowa, NJ (USA), 117–140, https://doi.org/10.1007/978-1-....
Khan K.U., Zuberi A., Nazir S., Fernandes J.B.K., Jamil Z., Sarwar H., 2016. Effects of dietary selenium nanoparticles on physiological and biochemical aspects of juvenile Tor putitora. Turk. J. Zool. 40, 704–712, https://doi.org/10.3906/zoo-15....
Khan K.U., Zuberi A., Ullah I., Sajjad, 2015. Effects of graded level of dietary L-ascorbyl-2-polyphosphate on growth performance and some haematological indices of juvenile mahseer (Tor putitora). Int. J. Agric. Biol. 17, 821–827, https://doi.org/10.17957/IJAB/....
Khazraie S.K., Ghazanfarpoor R., 2015. Effect of nano-selenium particles and sodium selenite on performance, glutathione peroxidase and superoxide dismutase of quail under heat stress. Int. J. Rev. Life. Sci. 5, 875–882.
Levkut M., Revajová V., Levkutová M., Ševčíková Z., Herich R., Borutová R., Leng L., 2009. Leukocytic responses of broilers following dietary contamination with deoxynivalenol and/or treatment by dietary selenium supplementation. Br. Poult. Sci. 50, 181–187, https://doi.org/10.1080/000716....
Mohamed H.S., Risk Y.S., Elslamony A.E., Soliman A.A., Ebrahim A.F., 2016. Study the relationship between selenium and heat shock proteins under heat stress for local Sinai chickens strain. Egypt. Poult. Sci. J. 36, 337–354, https://doi.org/10.21608/epsj.....
Mohapatra P., Swain R.K., Mishra S.K., Behera T., Swain P., Behura N.C., Sahoo G., Sethy K., Bhol B.P., Dhama K., 2014. Effects of dietary nano-selenium supplementation on the performance of layer grower birds. Asian J. Anim. Vet. Adv. 9, 641–652, https://doi.org/10.3923/ajava.....
Mountzouris K.C., Tsitrsikos P., Palamidi I., Arvaniti A., Mohnl M., Schatzmayr G., Fegeros K., 2010. Effects of probiotic inclusion levels in broiler nutrition on growth performance, nutrient digestibility, plasma immunoglobulins, and cecal microflora composition. Poult. Sci. 89, 58–67, https://doi.org/10.3382/ps.200....
NRC (National Research Council), 1994. Nutrient Requirements of Poultry. 9th Revised Edition. The National Academies Press. Washington, DC (USA), https://doi.org/10.17226/2114
Peng D., Zhang J., Liu Q., Taylor E.W., 2007. Size effect of elemental selenium nanoparticles (Nano-Se) at supranutritional levels on selenium accumulation and glutathione S-transferase activity. J. Inorg. Biochem. 101, 1457–1463, https://doi.org/10.1016/j.jino....
Pilarczyk B., Jankowiak D., Tomza-Marciniak A., Pilarczyk R., Sablik P., Drozd R., Tylkowska A.,Skólmowska M., 2012. Selenium concentration and glutathione peroxidase (GSHPx) activity in serum of cows at different stages of lactation. Biol. Trace Elem. Res. 147, 91–96, https://doi.org/10.1007/s12011....
Radwan N.L., Salah Eldin T.A., EL-Zaiat A.A., Mostafa A.S.A., 2015. Effect of dietary nano-selenium supplementation on selenium content and oxidant stability in table eggs and productive performance of laying hens. Int. J. Poult. Sci. 14, 161–176, https://doi.org/10.3923/ijps.2....
Raza A., 2012. Effects of graded levels of dietary selenium supplementation on the growth of juvenile mahseer (Tor Putitora). MPhil. Thesis. Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad (Pakistan).
Rozbicka-Wieczorek A.J., Szarpak E., Brzóska F., Śliwiński B., Kowalczyk J., Czauderna M., 2012. Dietary lycopenes, selenium compounds and fish oil affect the profile of fatty acids and oxidative stress in chicken breast muscle. J. Anim. Feed Sci. 21, 705–724, https://doi.org/10.22358/jafs/....
Saleh A.A., 2014. Effect of dietary mixture of Aspergillus probiotic and selenium nano-particles on growth, nutrient digestibilities, selected blood parameters and muscle fatty acid profile in broiler chickens. Anim. Sci. Pap. Rep. 32, 65–79.
Selim N.A., Radwan N.L., Youssef S.F., Salah Eldin T.A., Abo Elwafa S., 2015. Effect of inclusion inorganic, organic or nano selenium forms in broiler diets on: 2-Pysiological, immunological and toxicity statuses of broiler chicks. Int. J. Poult. Sci. 14, 144–155, https://doi.org/10.3923/ijps.2....
Suraï P.F., Dvorska J.E., 2002. Effect of selenium and vitamin E content of the diet on lipid peroxidation in breast muscle tissue of broiler breeder hens during storage. In: Proceeding of the Australian Poultry Science Symposium. Sydney (Australia), 14, 187–192.
Swain B.K., Johri T.S., Majumdar S., 2000. Effect of supplementation of vitamin E, selenium and their different combinations on the performance and immune response of broilers. Br. Poult. Sci. 41, 287–292, https://doi.org/10.1080/713654....
Wang H., Zhang J., Yu H., 2007. Elemental selenium at nano size possesses lower toxicity without compromising the fundamental effect on selenoenzymes: Comparison with selenomethionine in mice. Free Radical Biol. Med. 42, 1524–1533, https://doi.org/10.1016/j.free....
Yang Y.R., Meng F.C., Wang P., Jiang Y.B., Yin Q.Q., Chang J., Zuo R.Y., Zheng Q.H., Liu J.X., 2012. Effect of organic and inorganic selenium supplementation on growth performance, meat quality and antioxidant property of broilers. Afr. J. Biotechnol. 11, 3031–3036, https://doi.org/10.5897/AJB11.....
Zhang J., Wang X., Xu T., 2008. Elemental selenium at nano size (Nano-Se) as a potential chemopreventive agent with reduced risk of selenium toxicity: comparison with Semethylselenocysteine in mice. Toxicol. Sci. 101, 22–31, https://doi.org/10.1093/toxsci....
Zhou X., Wang Y., 2011. Influence of dietary nano elemental selenium on growth performance, tissue selenium distribution, meat quality, and glutathione peroxidase activity in Guangxi Yellow chicken. Poult. Sci. 90, 680–686, https://doi.org/10.3382/ps.201....
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