Integrated proteomics and biological assays for
characterization of non-nutritional effects
of butyric acid on intestinal cells

The non-nutritional effects of butyric acid on human intestinal epithelial cells were characterized by integration of biological assays and 2-dimensional gel-based proteomics for characterization of cellular protein expression. Human colonic adenocarcinoma (Caco-2) cells were cultured with sodium butyrate (NaB), foetal calf serum (fast-growing cells) or basal medium alone (control cells). Biological assays showed that butyric acid decreased cell proliferation and induced apoptosis. Principal component analysis of proteomic results showed that protein expression in NaB-treated cells was different from both control and fast-growing cells. Significantly different expressed proteins were characterized by mass spectrometry.

CITATIONS (2):

Creatine-induced activation of antioxidative defence in myotube cultures revealed by explorative NMR-based metabonomics and proteomics
Jette F Young, Lotte B Larsen, Anders Malmendal, Niels Chr Nielsen, Ida K Straadt, Niels Oksbjerg, Hanne C Bertram
Journal of the International Society of Sports Nutrition

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Effect of growth factors, estradiol 17-β, and short chain fatty acids on the intestinal HT29-MTX cells
Carlotta Giromini, Antonella Baldi, Eleonora Fusi, Raffaella Rebucci, Stig Purup
Cell Biology and Toxicology

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1230-1388

Assigning DOI numbers, introducing articles from supplements and recent publications to POL-index database, maintaining anti-plagiarism detection, electronic system to proceed manuscripts and webpage of the Journal with interactive pdf files, and language correction of manuscripts published in Journal of Animal and Feed Sciences are financed in the years 2018–2019 by the Ministry of Science and Higher Education from the funds for science popularization activities, Agreement No. 631/P-DUN/2018.