Effects of methionine and its ratio to lysine on expression of α s1 casein gene in cultured bovine mammary epithelial cells

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Policies General information Open Access, Licensing terms, Commercial use and Copyright terms policies Self-archiving policy and Archive policies Article Correction and Withdrawal policy Manuscript Submission policy Authorship policy Conflict of Interest policy Language considerations policy Plagiarism and Duplicate publications policy Ethics policy Review process policy Acceptance of manuscripts policy Online First Articles and Special Issues policies Generative artificial intelligence (AI) policy Advertising policy

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Suppl. 2/2007 vol. 16

SHORT COMMUNICATION

Effects of methionine and its ratio to lysine on expression of α_s1 casein gene in cultured bovine mammary epithelial cells

H. Y. Liu ¹

,

J. Y. Yang ¹

,

H. H. Wu ¹

,

Y. M. Wu ¹

,

J. X. Liu ¹

1

Institute of Dairy Science, Ministry of Education Key Laboratory of Molecular Animal Nutrition, Zhejiang University, Hangzhou 310029, P. R. China

Publication date: 2007-09-17

Corresponding author

Y. M. Wu

Institute of Dairy Science, Ministry of Education Key Laboratory of Molecular Animal Nutrition, Zhejiang University, Hangzhou 310029, P. R. China

J. X. Liu

Institute of Dairy Science, Ministry of Education Key Laboratory of Molecular Animal Nutrition, Zhejiang University, Hangzhou 310029, P. R. China

J. Anim. Feed Sci. 2007;16(Suppl. 2):330-334

DOI: https://doi.org/10.22358/jafs/74520/2007

KEYWORDS

mammary epithelial cell

αs1 casein gene expression

ABSTRACT

Effects of methionine dose and its ratio to lysine on α_s1 casein gene expression were evaluated in cultured bovine mammary epithelial cells. Methionine was added at levels of 0-100 μg/ml and ratio of lysine to methionine was set at 1-4.5:1. Expression of α_s1 casein gene was enhanced by addition of methionine up to 60 μg/ml, and then leveled off. There was an increasing trend in α_s1 casein expression with the increasing ratio of lysine to methionine and the highest level was at 3.5:1. These results indicated that adequate quantities and ratio of amino acids are required to promote milk protein synthesis.

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Stem Cells in Veterinary Science
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Min Yang, Xinyu Zhang, Yu Ding, Lewen Xie, Yu Gao, Kangyu Yao, Wanping Ren, Liang Yang, Yankun Zhao, Wei Shao
Frontiers in Veterinary Science

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