ORIGINAL PAPER
The influence of lipoprotein lipase gene polymorphism in Czech East Friesian sheep on the fatty acid profile in milk and yoghurt drinks – a preliminary study
1
Institute of Animal Science, Přátelství 815, 10400 Prague Uhříněves, Czech Republic
2
Dairy Research Institute, Ke Dvoru 12a, 160 00 Prague, Czech Republic
Publication date: 2021-03-12
Corresponding author
J. Anim. Feed Sci. 2021;30(1):52-57
KEYWORDS
TOPICS
ABSTRACT
The aim of the study was to evaluate the influence of single nucleotide polymorphism (SNP) in lipoprotein lipase gene (LPL) on the basic sheep milk parameters and the fatty acid profile in milk and yoghurt drinks made from it. The Primer Extension Analysis was used for genotyping 3 SNPs at positions: 74 (T/C), 130 (T/C), and 133 (T/A) in the 3´UTR region of the ovine LPL gene in 139 sheep of the East Friesian breed. According to the LPL genetic marker (CCCCAA and CTCTAT) 40 sheep were selected and divided into groups. Pooled milk was collected from May to June and was used to make yoghurt drinks with probiotics and prebiotics according to the genetic combination. In the pooled samples used for yoghurt production, the contents of fat, protein, lactose and total solids have been determined. The profile of fatty acid in fat from milk pooled samples as well as from yoghurt drinks has been analysed. Differences in the composition of milk and fatty acid profile were shown. The content of fat and total solids was significantly higher in the milk with the CTCTAT genotype combination in comparison to that with CCCCAA genotype. Moreover, these samples of milk and yoghurt products had a statistically lower content of palmitic acid and a significantly lower content of hypocholesterolemic acids. Targeted production of sheep milk from animals with different genotypes of the LPL may positively influence the fatty acid profile in milk and products made from it.
FUNDING
This research was supported by the Ministry of Agriculture of the Czech Republic, institutional support MZE-RO0718 (V001) and MZE-RO1420.
CONFLICT OF INTEREST
The authors declare that there is no conflict of interest.
REFERENCES (26)
1.
Barber M.C., Clegg R.A., Travers M.T., Vernon R.G., 1997. Lipid metabolism in the lactating mammary gland. Biochim. Biophys. Acta 1347, 101–126, https://doi.org/10.1016/S0005-....
2.
Bauman D.E., Griinari J.M., 2003. Nutritional regulation of milk fat synthesis. Ann. Rev. Nutr. 23, 203–227, https://doi.org/10.1146/annure....
3.
Bohačenko I., Pinkrová J., 2014. Fructan content determination by HPLC method with refractomeric detection. Listy Cukrov. Repar. 130, 28–32.
4.
Bonnet M., Leroux C., Chilliard Y., Martin P., 2000. Rapid communication: Nucleotide sequence of the ovine lipoprotein lipase cDNA. J. Anim. Sci. 78, 2994–2995, https://doi.org/10.2527/2000.7....
5.
Borková M., Šulc M., Novotná K., Smolová J., Hyršlová I., Fantová M., Elich O., 2018. The influence of feed supplementation with linseed oil and linseed extrudate on fatty acid profile in goat yoghurt drinks. Mljekarstvo 68, 30–36, https://doi.org/10.15567/mljek....
6.
Bouwman A.C., Bovenhuis H., Visker M.H.P.W., van Arendonk J.A.M., 2011. Genome-wide association of milk fatty acids in Dutch dairy cattle. BMC Genet. 12, 43, https://doi.org/10.1186/1471-2....
7.
Buccioni A., Serra A., Minieri S., Mannelli F., Cappucci A., Benvenuti D., Rapaccini S., Conte G., Mele M., 2015. Milk production, composition, and milk fatty acid profile from grazing sheep fed diets supplemented with chestnut tannin extract and extruded linseed. Small Rumin. Res. 130, 200–207, https://doi.org/10.1016/j.smal....
8.
Chilliard Y., Ferlay A., Rouel J., Lamberet G., 2003. A review of nutritional and physiological factors affecting goat milk lipid synthesis and lipolysis. J. Dairy Sci. 86, 1751–1770, https://doi.org/10.3168/jds.S0....
9.
Crisà A., Marchitelli C., Pariset L., Contarini G., Signorelli F., Napolitano F., Catillo G., Valentini A., Moioli B., 2010. Exploring polymorphisms and effects of candidate genes on milk fat quality in dairy sheep. J. Dairy Sci. 93, 3834–3845, https://doi.org/10.3168/jds.20....
10.
ČSN EN ISO 1211, 2011. Milk – Determination of fat content – Gravimetric method (Reference method). Czech Office for Standards, Metrology and Testing. Prague (Czech Republic).
11.
ČSN ISO 20128, 2007. Milk products – Enumeration of presumptive Lactobacillus acidophilus on a selective medium – Colony-count technique at 37 °C. Czech Office for Standards, Metrology and Testing. Prague (Czech Republic).
12.
ČSN ISO 29981, 2010. Milk products – Enumeration of presumptive bifidobacteria – Colony-count technique at 37 °C. Czech Office for Standards, Metrology and Testing. Prague (Czech Republic).
13.
ČSN ISO 7889, 2004. Yoghurt – Enumeration of characteristic microorganisms – Colony-count technique at 37 °C. Czech Office for Standards, Metrology and Testing. Prague (Czech Republic).
14.
Hidayati H., Sumanti C., Noor R.R., Priyanto R., Rahayu S., 2015. Single nucleotide polymorphisms of lipoprotein lipase gene and its association with marbling quality in local sheeps. J. Indonesian Trop. Anim. Agric. 40, 1–10, https://doi.org/10.14710/jitaa....
15.
Korbie D.J., Mattick J.S., 2008. Touchdown PCR for increased specificity and sensitivity in PCR amplification. Nat. Protoc. 3, 1452–1456, https://doi.org/10.1038/nprot.....
16.
Markiewicz-Kęszycka M., Czyżak-Runowska G., Lipińska P., Wójtowski J., 2013. Fatty acid profile of milk – a review. Bull. Vet. Inst. Pulawy 57, 135–139, https://doi.org/10.2478/bvip-2....
17.
Marchitelli C., Contarini G., De Matteis G., Crisà A., Pariset L., Scatà M. C., Catillo G., Napolitano F., Moioli B., 2013. Milk fatty acid variability: effect of some candidate genes involved in lipid synthesis. J. Dairy Res. 80, 165–173, https://doi.org/10.1017/S00220....
18.
Moioli B., Contarini G., Pariset L., Marchitelli C., Crisà A., Catillo G., Napolitano F., 2012. Genetic variation of C18:1 and C18:2 isomers in sheep milk fat. Small Rumin. Res. 103, 187–193, https://doi.org/10.1016/j.smal....
19.
Ogorevc J., Kunej T., Razpet A., Dovc P., 2009. Database of cattle candidate genes and genetic markers for milk production and mastitis. Anim. Genet. 40, 832–851, https://doi.org/10.1111/j.1365....
20.
Palmquist D.L., 2006. Milk fat: origin of fatty acids and influence of nutritional factors thereon. In: P.F. Fox, P.L.H. McSweeney (Editors). Advanced Dairy Chemistry Volume 2 Lipids. Springer. Boston, MA (USA), pp 43–92, https://doi.org/10.1007/0-387-....
21.
Regulation No. 397/2016 Coll. on requirements for milk and milk products, frozen creams and edible fats and oils (Vyhláška č. 397/2016 Sb. o požadavcích na mléko a mléčné výrobky, mražené krémy a jedlé tuky a oleje).
22.
Reynolds C.K., Cannon V.L., Loerch S.C., 2006. Effects of forage source and supplementation with soybean and marine algal oil on milk fatty acid composition of ewes. Anim. Feed Sci. Technol. 131, 333–357, https://doi.org/10.1016/j.anif....
23.
Sánchez J.P., San Primitivo F., Barbosa E., Varona L., de la Fuente L.F., 2010. Genetic determination of fatty acid composition in Spanish Churra sheep milk. J. Dairy Sci. 93, 330–339, https://doi.org/10.3168/jds.20....
24.
Shingfield K.J., Bonnet M., Scollan N.D., 2013. Recent developments in altering the fatty acid composition of ruminant-derived foods. Animal 7, 132–162, https://doi.org/10.1017/S17517....
25.
Ulbricht T.L.V., Southgate D.A.T., 1991. Coronary heart disease: seven dietary factors. Lancet 38, 985–992, https://doi.org/10.1016/0140-6....
26.
Untergasser A., Cutcutache I., Koressaar T., Ye J., Faircloth B.C., Remm M., Rozen S.G., 2012. Primer3 - new capabilities and interfaces. Nucleic Acids Res. 40, e115, http://doi.org/10.1093/nar/gks....
Share
RELATED ARTICLE
| ISSN: | 1230-1388 |
Assigning DOI numbers, introducing articles from supplements and recent publications to POL-index database, maintaining anti-plagiarism detection, electronic system to proceed manuscripts and webpage of the Journal with interactive pdf files, and language correction of manuscripts published in Journal of Animal and Feed Sciences are financed in the years 2018–2019 by the Ministry of Science and Higher Education from the funds for science popularization activities, Agreement No. 631/P-DUN/2018.
© 2006-2024 Journal hosting platform by Bentus
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
