CC-BY 4.0

The effects of type of protein and fibre fermented in vitro with different pig inocula on short-chain fatty acids and amines concentrations

M. Taciak 1  ,  
M. Barszcz 1,  
A. Tuśnio 1,  
I. Bachanek 1,  
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
J. Anim. Feed Sci. 2015;24(3):235–243
Publish date: 2015-09-08
In an in vitro experiment with pig inoculum, the main and interactive effects of type of protein and fibre on the concentrations of short-chain fatty acids (SCFA) and amines as the end products of fermentation and proteolysis were determined. The effects of inoculation with colonic digesta (C) sampled post mortem or with freshly voided faeces (F) as the sources of microflora, and of 24- vs 48-h periods of incubation, were also estimated. Potato protein and casein were fermented each with cellulose, pectin, or raw potato (resistant) starch. Each set of fermentation was repeated six times. The total SCFA concentration was greater after C than F fermentation, regardless of the substrates. It was the greatest after fermentation of both proteins with potato starch and greater after fermentation of all carbohydrates with casein than with potato protein. The largest concentrations of isobutyric and isovaleric acids were found after fermentation of casein with cellulose, but only with C (P = 0.040 and P = 0.030 for interaction, respectively). Also, a butyrogenic effect of potato starch was found only after C fermentation and was greater after 48 than 24 h. The total amines concentration after C and F fermentation of potato protein with all fibres was the same, while after C fermentation of casein it was affected by the type of fibre (P = 0.001 and P = 0.000 after 24 and 48 h, respectively). It was very high with cellulose, lower with starch and the lowest with pectin. It may be concluded that in vitro bacterial proteolysis is greatly affected by the interaction of type of protein and fibre. Forty-eight-hour fermentation with colonic digesta seems to be more discriminative than 24-h fermentation and colonic digesta is a more discriminative pig inoculum than faeces.
M. Taciak   
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
1. Effect of Replacing Soybean Meal by Raw or Extruded Pea Seeds on Growth Performance and Selected Physiological Parameters of the Ileum and Distal Colon of Pigs
Anna Tuśnio, Marcin Taciak, Marcin Barszcz, Ewa Święch, Ilona Bachanek, Jacek Skomiał, Gunnar Loh
2. Chemopreventive Potential of Powdered Red Wine Pomace Seasonings against Colorectal Cancer in HT-29 Cells
Raquel Del Pino-García, María D. Rivero-Pérez, María L. González-SanJosé, Miriam Ortega-Heras, Javier García Lomillo, Pilar Muñiz
Journal of Agricultural and Food Chemistry
3. Interactive effects of protein and carbohydrates on production of microbial metabolites in the large intestine of growing pigs
Marcin Taciak, Marcin Barszcz, Ewa Święch, Anna Tuśnio, Ilona Bachanek
Archives of Animal Nutrition
4. Glycation of fish protein impacts its fermentation metabolites and gut microbiota during in vitro human colonic fermentation
Yuhong Yang, Haohao Wu, Shiyuan Dong, Weiya Jin, Kaining Han, Yanmei Ren, Mingyong Zeng
Food Research International
5. The effect of dietary level of two inulin types differing in chain length on biogenic amine concentration, oxidant-antioxidant balance and DNA repair in the colon of piglets
Marcin Barszcz, Marcin Taciak, Anna Tuśnio, Ewa Święch, Ilona Bachanek, Paweł Kowalczyk, Andrzej Borkowski, Jacek Skomiał, Juan J. Loor
6. The potential of pectin to impact pig nutrition and health: feeding the animal and its microbiome
Maria Wiese
FEMS Microbiology Letters