ORIGINAL PAPER
The effect of intracerebroventricular infusion of leptin on the secretory activity of the somtotropic axis in fasted prepubertal lambs
 
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1
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
2
Poznań University of Life and Sciences, Department of Animal Physiology and Biochemistry Wojska Polskiego 28, 60-637 Poznań, Poland
CORRESPONDING AUTHOR
A. Wójcik-Gładysz   

The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
Publication date: 2010-08-16
 
J. Anim. Feed Sci. 2010;19(3):379–397
 
KEYWORDS
ABSTRACT
The aim of the present study was to investigate the effect of exogenous leptin on somatotrophic axis regulation in prepubertal female lambs under conditions of acute undernutrition (72 h fasting). The analyses in fasted sheep revealed enhancement of immunoreactive (ir) somatostatin stores in perikarya of the periventricular (PEV) nucleus and in terminals of the median eminence (ME) (P<0.001), enhancement of growth hormone (GH) mRNA expression in the adenohypophysis, diminishing of ir GH stores in somatotrophs in the adenohypophysis (P<0.001), and a rise in GH pulsatility (P<0.05) in the blood in comparison with standard-fed sheep. In fasted sheep infused with leptin, additional enhancement of ir somatostatin stores in the PEV nucleus and in the ME (P<0.001), an increase in GH mRNA expression in the adenohypophysis (P<0.001), and augmentation of GH pulsatility (P<0.05) in the blood plasma were noted. In conclusion, acute undernutrition affects somatotrophic axis by enhancement of GH secretion via restraining of somatostatin output. Exogenous leptin additionally amplifies this effect by suppressing somatostatin release and increasing of GH secretion. The results provide evidence that leptin can regulate somatotrophic axis activity in prepubertal female lambs under conditions of acute fasting.
 
CITATIONS (3):
1.
The effect of intracerebroventricular infusions of ghrelin and/or short fasting on the gene expression and immunoreactivity of somatostatin in the hypothalamic neurons and on pituitary growth hormone in prepubertal female lambs. Morphological arguments
Jolanta Polkowska, Marta Wańkowska, Katarzyna Romanowicz, Alina Gajewska, Tomasz Misztal, Anna Wójcik-Gładysz
Brain Research
 
2.
Obestatin stimulates the somatotrophic axis activity in sheep
Anna Wójcik-Gładysz, Michał Szlis, Anna Misztal, Bartosz Jarosław Przybył, Jolanta Polkowska
Brain Research
 
3.
Does obestatin modulate the hypothalamic appetite-regulating network in peripubertal sheep?
M. Szlis, J. Polkowska, E. Skrzeczyńska, B. J. Przybył, A. Wójcik-Gładysz
Journal of Animal Physiology and Animal Nutrition
 
ISSN:1230-1388