0.906
IF5
0.875
IF
Q3
JCR
1.0
CiteScore
0.374
SJR
Q2
SJR
20
MNiSW
165.24
ICV
REVIEW PAPER
 
CC-BY 4.0
 
 

RT-PCR technique and its applications. State-of the-art

T. Malewski 1,  
A. Malewska 2,  
 
1
Institute of Genetic and Animal Breeding, Polish Academy of Sciences, Jastrzębiec, 05-552 Wólka Kosowska, Poland
2
Muzeum and Institute of Zoology, Polish Academy of Sciences, Wilcza 64, 00-679 Warsaw, Poland
J. Anim. Feed Sci. 2003;12(3):403–416
Publication date: 2003-07-15
KEYWORDS
ABSTRACT
The reverse transcription polymerase chain reaction (RT-PCR) is one of the most sensitive methods for the detection and quantitation of mRNA. It is widely used for quantification of mRNA levels and is useful tool for basic research, agriculture, medicine and biotechnology. Introduction of Real-time technique significantly improves rapidity, sensitivity, specificity and reproducibility of RT-PCR method. Real-time PCR detects and quantifies nucleic acids even from live and dead pathogens or cells. This review discusses the background, advantages and limitations of conventional and Real-time RT-PCR methods for quantitation of gene expression and its application in basic, agricultural and biomedical research.
 
CITATIONS (1):
1. Development of an Assay to Quantify Rumen Ciliate Protozoal Biomass in Cows Using Real-Time PCR
John T. Sylvester, Sanjay K. R. Karnati, Zhongtang Yu, Mark Morrison, Jeffrey L. Firkins
The Journal of Nutrition
ISSN:1230-1388