0.857
IF5
0.900
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0.92
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MNiSW
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ICV
ORIGINAL PAPER
 
CC-BY 4.0
 
 

Probe generated by Y chromosome microdissection is useful for analysing the sex chromosomes of the domestic horse

M. Bungo 2  ,  
C. Schelling 3,  
 
1
Department of Genetics and Animal Breeding, August Cieszkowski Agricultural University, Wołyńska 33, 60-637 Poznań, Poland
2
Department of Immuno- and Cytogenetics, National Research Institute of Animal Production, 32-083 Balice, Poland
3
Swiss Federal Institute of Technology, Zurich and Vetsuisse-Faculty, University of Zurich, Department of Animal Sciences, Tannenstrasse 1, 8092 Zurich, Switzerland
J. Anim. Feed Sci. 2006;15(2):173–178
Publish date: 2006-04-12
KEYWORDS
ABSTRACT
Microdissection of chromosomes is an invaluable tool for physical isolation of single chromosomes, chromosome arms or chromosome bands. In the present study we have generated a fluorescence in situ hybridization (FISH) probe from the microdissected Y chromosome of the domestic horse. For the identification of the Y chromosome, we used the CBG technique. A biotinlabelled probe in the FISH technique gave a specific signal on all Y chromosomes and an additional signal in the interstitial part of the long arm of the X chromosome. This area showed an additional C band detected by the CBG method and characteristic for this chromosome. The probe obtained can therefore be used for analysis of the Y and X chromosomes and their copies in the genome, and, in the case of the X chromosome, also for gene mapping.
CORRESPONDING AUTHOR
M. Bungo   
Department of Immuno- and Cytogenetics, National Research Institute of Animal Production, 32-083 Balice, Poland
 
CITATIONS (2):
1. Sex reversal syndrome in the horse: Four new cases of feminization in individuals carrying a 64,XY SRY negative chromosomal complement
Gabriel Anaya, Miguel Moreno-Millán, Monika Bugno-Poniewierska, Klaudia Pawlina, Alberto Membrillo, Antonio Molina, Sebastián Demyda-Peyrás
Animal Reproduction Science
2. First case of sterility associated with sex chromosomal abnormalities in a jenny
J Dorado, G Anaya, M Bugno-Poniewierska, A Molina, A Mendez-Sanchez, I Ortiz, M Moreno-Millán, M Hidalgo, P Peral García, S Demyda-Peyrás
Reproduction in Domestic Animals
ISSN:1230-1388