Probe generated by Y chromosome microdissection is useful for analysing the sex chromosomes of the domestic horse
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Department of Genetics and Animal Breeding, August Cieszkowski Agricultural University, Wołyńska 33, 60-637 Poznań, Poland
Department of Immuno- and Cytogenetics, National Research Institute of Animal Production, 32-083 Balice, Poland
Swiss Federal Institute of Technology, Zurich and Vetsuisse-Faculty, University of Zurich, Department of Animal Sciences, Tannenstrasse 1, 8092 Zurich, Switzerland
Publication date: 2006-04-12
Corresponding author
M. Bungo   

Department of Immuno- and Cytogenetics, National Research Institute of Animal Production, 32-083 Balice, Poland
J. Anim. Feed Sci. 2006;15(2):173-178
Microdissection of chromosomes is an invaluable tool for physical isolation of single chromosomes, chromosome arms or chromosome bands. In the present study we have generated a fluorescence in situ hybridization (FISH) probe from the microdissected Y chromosome of the domestic horse. For the identification of the Y chromosome, we used the CBG technique. A biotinlabelled probe in the FISH technique gave a specific signal on all Y chromosomes and an additional signal in the interstitial part of the long arm of the X chromosome. This area showed an additional C band detected by the CBG method and characteristic for this chromosome. The probe obtained can therefore be used for analysis of the Y and X chromosomes and their copies in the genome, and, in the case of the X chromosome, also for gene mapping.
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