1.054
IF5
1.150
IF
Q3
JCR
1.7
CiteScore
0.396
SJR
Q2
SJR
40
MNiSW
148.75
ICV
ORIGINAL PAPER
 
CC-BY 4.0
 
 

Novel polymorphisms detected in the prolactin receptor gene of Yili horse (Equus caballus) by PCR-SSCP

R. Abula 1, 2,  
H.-L. Zhang 1,  
Y. Chen 1  ,  
 
1
Xinjiang Key Laboratory of Meat- and Milk-Production, Herbivore Nutrition, College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, P. R. China
2
Chemical and Environmental Science Department, Kashgar Teacher’s College, Kashgar 844006, P. R. China
J. Anim. Feed Sci. 2013;22(1):70–76
Publication date: 2013-03-18
KEYWORDS
ABSTRACT
The prolactin receptor (PRLR) is a receptor for a polypeptide hormone that stimulates mammary gland development and lactation in mammals. Associations between the PRLR genotype and cow and goat milk traits have been reported. To date, the nucleotide polymorphisms within the PRLR gene in equine species have not been investigated. Single-nucleotide polymorphism (SNP) is the most abundant form of genetic variation and a resource for useful genetic traits. In the present study, SNPs within the coding regions of the PRLR gene were detected by polymerase chain reaction-single-strand conformation polymorphism (SSCP) and DNA sequencing on the Yili horse of China. Ten novel SNPs were identified in the PRLR gene: one located within exon 4 (XM_001500104: c.156 C>T); one within exon 5 (c.312 T>C); and eight within exon 10 (c.1062 T>C, c.1083 G>A, c.1226 A>C, c.1233 C>T, c.1279 C>A, c.1749 A>T, c.1764 G>C, and c.1778 T>A). The mutation allele frequencies and effective allele numbers ranged from 0.10 to 0.30 and from 1.2195 to 1.7240, respectively. Four of the identified SNPs are non-synonymous and involve four amino acid substitutions, namely, p.H409P (c.1226 A>C), p.H427N (c.1279 C>A), p.Q588H (c.1764 G>C), and p.I593K (c.1778 T>A), in the cytoplasmic domain of the PRLR, which plays a fundamental role in signal transduction. Theoretically, the four nucleotide substitutions would result in altered spatial structure and functions of the PRLR molecules. Further study is required, however, to determine whether these mutations affect the milk yield of the Yili horse.
CORRESPONDING AUTHOR
Y. Chen   
Xinjiang Key Laboratory of Meat- and Milk-Production, Herbivore Nutrition, College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, P. R. China
ISSN:1230-1388