ORIGINAL PAPER
Preliminary study of the effects of Apilactobacillus kunkeei
EIR/BG-1 and Enterococcus hirae EIR/CM-2 supplementation
on in vitro rumen fermentation and microbial population
1
Burdur Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Physiology, 15030 Burdur, Turkey
2
Ankara University, Faculty of Veterinary Medicine, Department of Pharmacology and Toxicology, 06110 Ankara, Turkey
3
Ankara University, Faculty of Veterinary Medicine, Department of Physiology, 06110 Ankara, Turkey
4
Burdur Mehmet Akif Ersoy University, Scientific and Technology Application and Research Center, 15030 Burdur, Turkey
5
Ankara University, Faculty of Agriculture, Department of Animal Science, 06110 Ankara, Turkey
6
Ankara University, Faculty of Science, Department of Biology, Pharmabiotic Technologies Research Laboratory, 06100 Ankara, Turkey
Publication date: 2023-10-20
Corresponding author
A. Demirtas
Burdur Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Physiology, 15030 Burdur, Turkey
Burdur Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Physiology, 15030 Burdur, Turkey
J. Anim. Feed Sci. 2024;33(1):88-100
KEYWORDS
direct feed microorganismsfeed additiveslactic acid bacteriaprobioticsrumen bacteriaruminal fermentation
TOPICS
cattlefeed additivesmicrobiologymolecular biologynutritionprobiotics, prebiotics, symbiotics, eubiotics, antibioticsrumen fermentationphysiology
ABSTRACT
The aim of the study was to assess the impact of potential probiotic
strains of lactic acid bacteria (LAB), Apilactobacillus kunkeei EIR/BG-1 isolated
from the gut of honeybees, and Enterococcus hirae EIR/CM-2 isolated from
cow’s milk, on rumen fermentation parameters and microbial population in a high-concentrate
diet using a rumen simulation technique (Rusitec). The experiment
consisted of 7 days of adaptation and 7 days of data collection. The dietary
treatments were as follows: no additives (control), addition of 1 ml/fermenter (108
CFU) of Al. kunkeei EIR/BG-1, and addition of 1 ml/fermenter (108 CFU) of E.
hirae EIR/CM-2. Alongside rumen fermentation characteristics, rumen microbial
composition was investigated using real-time PCR. Supplementation with LAB
strains did not affect ruminal pH, production of methane, and total and individual
short chain fatty acids, ammonia-N concentration, dry matter digestibility
and total protozoa. However, the abundance of Ruminococcus flavefaciens
increased in the Al. kunkeei EIR/BG-1 treatment (P < 0.05). Moreover, the size
of R. flavefaciens population in E. hirae EIR/CM-2 was comparable to that in
the Al. kunkeei EIR/BG-1 treatment. Selenomonas ruminantium was more
abundant in the Al. kunkeei EIR/BG-1 treatment compared to the E. hirae
EIR/CM-2 treatment (P < 0.05). The abundance of Streptococcus bovis and
Megasphaera elsdenii decreased with both Al. kunkeei EIR/BG-1 and E. hirae
EIR/CM-2 supplementations (P < 0.05). In conclusion, Al. kunkeei EIR/BG-1
and E. hirae EIR/CM-2 exhibited beneficial effects on some members of the
rumen microbial population, although these effects did not manifest in significant
alterations in ruminal fermentation. Further research is required to clarify the
probiotic potentials of these LAB strains as feed additives for ruminant rations.
CONFLICT OF INTEREST
The Authors declare that there is no conflict of
interest.
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