ORIGINAL PAPER
Integrated proteomics and biological assays for
characterization of non-nutritional effects
of butyric acid on intestinal cells
1
University of Aarhus, Faculty of Agricultural Sciences,
Department of Animal Health, Welfare and Nutrition, P.O. Box 50, DK-8830 Tjele, Denmark
2
University of Aarhus, Faculty of Agricultural Sciences, Department of Food Science
P.O. Box 50, DK-8830 Tjele, Denmark
Publication date: 2007-01-26
Corresponding author
S. Purup
University of Aarhus, Faculty of Agricultural Sciences, Department of Animal Health, Welfare and Nutrition, P.O. Box 50, DK-8830 Tjele, Denmark
University of Aarhus, Faculty of Agricultural Sciences, Department of Animal Health, Welfare and Nutrition, P.O. Box 50, DK-8830 Tjele, Denmark
J. Anim. Feed Sci. 2007;16(Suppl. 1):163-171
KEYWORDS
ABSTRACT
The non-nutritional effects of butyric acid on human intestinal epithelial cells were characterized
by integration of biological assays and 2-dimensional gel-based proteomics for characterization
of cellular protein expression. Human colonic adenocarcinoma (Caco-2) cells were cultured with
sodium butyrate (NaB), foetal calf serum (fast-growing cells) or basal medium alone (control cells).
Biological assays showed that butyric acid decreased cell proliferation and induced apoptosis.
Principal component analysis of proteomic results showed that protein expression in NaB-treated
cells was different from both control and fast-growing cells. Significantly different expressed
proteins were characterized by mass spectrometry.
CITATIONS (2):
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Jette F Young, Lotte B Larsen, Anders Malmendal, Niels Chr Nielsen, Ida K Straadt, Niels Oksbjerg, Hanne C Bertram
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2.
Effect of growth factors, estradiol 17-β, and short chain fatty acids on the intestinal HT29-MTX cells
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Cell Biology and Toxicology
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