Immunoreactive properties of pea protein extract and
its trypsin hydrolysates
1
Faculty of Biology, Chair of Biochemistry,Warmia and Mazury University,
Oczapowskiego 1A, 10-957 Olsztyn, Poland
2
Institute of Animal Reproduction and Food Research,
Polish Academy of Sciences,
Tuwima 10, 10-747 Olsztyn, Poland
Publication date: 2007-09-06
Corresponding author
H. Kostyra
Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland
Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland
J. Anim. Feed Sci. 2007;16(3):472-484
KEYWORDS
ABSTRACT
The study was undertaken to examine the immunogenic potential of pea protein of Polish
variety Rodan and its trypsin hydrolysates differing in degree of hydrolysis. The physicochemical
characteristic of pea protein extract and its hydrolysates, DH 2.0 and 5.0, were determined by
SDS-PAGE electrophoresis, chromatofocusing, affinity chromatography, and sequential analysis.
The immunogenic properties of pea protein and its trypsin hydrolysates, DH 2.0 and 5.0, were
investigated by direct and competitive ELISA methods. The results confirmed that protein extract is
a stronger immunogen than hydrolysates, while hydrolysate DH 2.0 was a stronger immunogen than
DH 5.0. The dominant antigen isolated from pea protein extract and both trypsin hydrolysates had a
molecular weight of about 20 kDa and was in the glycoprotein fraction. The N-terminal sequence of
this antigen was determined to be: Thr-Glu-Thr-Thr-Ser-Phe-Leu-Ile-Thr-Lys-.
CITATIONS (2):
1.
Enzymatic Hydrolysis and Fermentation of Pea Protein Isolate and Its Effects on Antigenic Proteins, Functional Properties, and Sensory Profile
Arteaga García, Victoria Demand, Karolin Kern, Andrea Strube, Michael Szardenings, Isabel Muranyi, Peter Eisner, Ute Schweiggert-Weisz
Foods
Arteaga García, Victoria Demand, Karolin Kern, Andrea Strube, Michael Szardenings, Isabel Muranyi, Peter Eisner, Ute Schweiggert-Weisz
Foods
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Avanços conceituais em nutrição humana: ciência e tecnologia de alimentos
Valdemiro Sgarbieri
Valdemiro Sgarbieri
| ISSN: | 1230-1388 |
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