The effect of enzyme supplementation was studied in a balanced three-period (3 x 13 days) crossover trial with 16 male piglets weaned on day 11 post-natum. On days 15 and 16 of age the piglets were fitted with a simple T-cannula at the distal ileum. Starting on day 21 post-natum, piglets were fed the basal diet (22.1% CP and 14.5 MJ ME/kg as fed) without or with the addition of xylanase (endo-1,4-β-xylanase, EC 3.2.1.8) at a level of 5600 EXU/kg (as fed) or phospholipase (phospholipase A2, EC 3.1.1.4) at a level of 1000 IU/kg (as fed), or a combination of both. Activities of maltase, lactase, leucine aminopeptidase (LAP), lipase and α-amylase were measured in ileal digesta. The supplementation with exogenous feed enzymes had no significant (P>0.05) effect on the activities of maltase, LAP, lipase and α-amylase. The activity of lactase tended (P=0.1) to be higher in piglets fed the diet supplemented with the combination of both enzymes compared to the basal diet. In conclusion, the addition of exogenous feed enzymes had only minor effects on the activity of piglets’ own enzymes in the chyme entering the hindgut.