ORIGINAL PAPER
Effects of oocyte quality and semen donor on the efficiency of in vitro embryo production in cattle
 
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1
Rzeszow University, Department of Bioengineering, Branch Campus of the Faculty of Biotechnology, 36-100 Kolbuszowa, Poland
2
National Research Institute of Animal Production, Department of Biotechnology of Animal Reproduction, 32-083 Balice, Poland
CORRESPONDING AUTHOR
L. Kątska-Książkiewicz   

Rzeszow University, Department of Bioengineering, Branch Campus of the Faculty of Biotechnology, 36-100 Kolbuszowa, Poland
Publication date: 2009-03-20
 
J. Anim. Feed Sci. 2009;18(2):257–270
 
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ABSTRACT
The aim of the study was to determine whether immature bovine oocytes selected by morphology and BCB test (brilliant cresyl blue) and used for in vitro embryo production would increase the blastocyst rate when inseminated with sperm derived from bulls of reduced in vitro fertilizability. To relate oocyte quality and bull effect to blastocyst quality, morphology, ability for hatching and caspase-3 activity (marker of apoptosis) were evaluated. Both, oocytes originating from BCB+ and from Control COCs exhibited higher cleavage and blastocyst rates (P<0.001) compared with BCB- oocytes. Significant differences in cleavage and blastocyst rates between the two bulls were observed. The highest blastocyst rate was noticed for BCB+ oocytes, regardless the bull. Comparison of the caspase-3 activity shows a significant overbalance of blastocysts with high caspase-3 activity, regardless the stage of blastocyst development and their origin. Thus, using excellent quality oocytes and sperm with reduced in vitro fertilizability it was possible to some extent to overcome bull effect on embryo production.
 
CITATIONS (4):
1.
Effect of Hyaluronan on Developmental Competence and Quality of Oocytes and Obtained Blastocysts fromIn VitroMaturation of Bovine Oocytes
Jolanta Opiela, Joanna Romanek, Daniel Lipiński, Zdzisław Smorąg
BioMed Research International
 
2.
Evaluation of changes arising in the pig mesenchymal stromal cells transcriptome following cryopreservation and Trichostatin A treatment
Artur Gurgul, Joanna Romanek, Klaudia Pawlina-Tyszko, Tomasz Szmatoła, Jolanta Opiela, Zoran Ivanovic
PLOS ONE
 
3.
Varied Approach of Using MSCs for Bovine Embryo In Vitro Culture
Jolanta Opiela, Bülent Bülbül, Joanna Romanek
Animal Biotechnology
 
4.
Varied approach of using MSCs for bovine embryo in vitro culture
Jolanta Opiela, Bülent Bülbül, Joanna Romanek
Animal Biotechnology
 
ISSN:1230-1388