ORIGINAL PAPER
Effect of vitamin E supplementation on serum alpha tocopherol and immune status of Murrah buffalo (Bubalus bubalis) calves
 
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Indian Veterinary Research Institute, Mineral and Vitamin Nutrition Laboratory, Centre of Advanced Studies in Animal Nutrition, Izatnagar-243122 (UP), India
CORRESPONDING AUTHOR
R. S. Dass   

Indian Veterinary Research Institute, Mineral and Vitamin Nutrition Laboratory, Centre of Advanced Studies in Animal Nutrition, Izatnagar-243122 (UP), India
Publication date: 2008-01-15
 
J. Anim. Feed Sci. 2008;17(1):19–29
 
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ABSTRACT
The study evaluated the effect of graded levels of vitamin E supplementation on the serum α-tocopherol, retinol concentrations and immune response of buffalo calves. In a completely randomized design, 15 male Murrah buffalo calves (14-15 months old; 194.0 ± 3.5 kg average body weight) were divided into 3 equal groups on the basis of their body weights. All the calves were fed on wheat straw and concentrate mixture to meet their nutrients requirements. In addition, all the calves in group II and III were supplemented with 300 and 600 IU of feed grade DL- α-tocopheryl acetate/d, respectively. Blood samples were collected at zero day and subsequently at 30 days intervals up to 120 days to estimate serum α-tocopherol and retinol concentration. To assess humoral immune response, all the buffalo calves were sensitized with formalin inactivated Pasteurella multocida antigen and blood was collected on 0, 7, 14, 21 and 28 days post vaccination to study antibody production using indirect ELISA, whereas cell mediated immune response of buffalo calves was assessed by in vivo delayed type hypersensitivity (DTH) reaction against phytohaemaglutinin-P (PHA-P). Retinol concentration (μg/ml) in blood serum of three groups of calves was statistically similar (P>0.05), whereas the cumulative group mean serum α-tocopherol concentration (μg/dl) was significantly (P<0.01) higher in vitamin E supplemented groups as compared to control group. The pooled mean ELISA antibody titer against Pasteurella multocida was also significantly (P<0.05) higher in buffalo calves given vitamin E as compared to control group calves, but cell mediated immune response was similar ((P>0.05) in three groups. Results of the present study indicated that vitamin E supplementation increased the α-tocopherol concentration in serum and also enhanced the humoral immune response of buffalo calves.
 
CITATIONS (2):
1.
Effect of supplemental vitamin E on the peripheral blood leukocyte population in Japanese Black calves
Konosuke OTOMARU, Shun SAITO, Karura ENDO, Masayuki KOHIRUIMAKI, Hiromichi OHTSUKA
Journal of Veterinary Medical Science
 
2.
Effect of Supplemental Vitamin E on Antibody Titer in Japanese Black Calves Vaccinated against Bovine Herpesvirus-1
Konosuke OTOMARU, Shun SAITO, Karura ENDO, Masayuki KOHIRUIMAKI, Shin-ichi FUKUYAMA, Hiromichi OHTSUKA
Journal of Veterinary Medical Science
 
ISSN:1230-1388