ORIGINAL PAPER
Activity and stability of a fungal 1,4-beta-endo-xylanase preparation in the rumen of sheep
Á. Bata 1,   J. Kutasi 1,   V. Jurkovich 2,   H. Fébel 3,   P. Rafai 2  
 
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1
Dr. Bata Biotechnological Research and Development Ltd., H-2364 Ócsa, Hungary
2
Szent István University, Faculty of Veterinary Science, Department of Animal Hygiene, Herd Health and Veterinary Ethology, István u 2., H-1078 Budapest, Hungary
3
Research Institute for Animal Breeding and Nutrition, Gesztenyés u 1., H-2053 Herceghalom, Hungary
CORRESPONDING AUTHOR
P. Rafai   

Szent István University, Faculty of Veterinary Science, Department of Animal Hygiene, Herd Health and Veterinary Ethology, István u 2., H-1078 Budapest, Hungary
Publication date: 2002-10-11
 
J. Anim. Feed Sci. 2002;11(4):627–635
 
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ABSTRACT
Experiments were conducted with 1-3 years old, rumen cannulated Merino wethers to characterize the stability of the 1,4-β-endo-xylanase activity of a commercially available enzyme preparation (Rumino-Zyme, 250 FXU/g xylanase activity, Dr. Bata Ltd., Hungary). The Remazol blue technique used for determination of the xylanase activity of the rumen fluid and solid samples proved simple, quick and reliable. The xylanase activity in the rumen of fasting wethers was found to be 20-30 FXU/l. It was by about 2.5 times greater two and four hours after feeding than the value measured pre-feeding. Exogenous xylanase added directly into the rumen of fasted wethers caused an increase in the enzyme activity. After the peak activity at 5 min after treatment the enzymatic activity decreased with an estimated half-life of 45-50 min. This result of the present investigation did not support the earlier observations, which indicated lasting activity of external NSP-enzymes in vivo.
 
CITATIONS (1):
1.
Rumen fermentation response to a direct-fed xylanase enzyme preparation fromThermomyces lanuginosusin sheep
V. Jurkovich, J. Kutasi, Hedvig Fébel, J. Reiczigel, E. Brydl, L. Könyves, P. Rafai
Acta Veterinaria Hungarica
 
ISSN:1230-1388