ORIGINAL PAPER
A note on sampling baled grass silage for fungal propagules
M. O’Brien 1, 2  
,   P. O’Kiely 1,   P. D. Forristal 3,   H. T. Fuller 2
 
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1
Teagasc, Grange Beef Research Centre, Dunsany, Co Meath, Ireland
2
UCD School of Biology and Environmental Science, College of Life Sciences, University College Dublin, Belfield, Dublin 4
3
Teagasc, Crops Research Centre, Oak Park, Co Carlow, Ireland
CORRESPONDING AUTHOR
M. O’Brien   

Teagasc, Grange Beef Research Centre, Dunsany, Co Meath, Ireland; UCD School of Biology and Environmental Science, College of Life Sciences, University College Dublin, Belfield, Dublin 4
Publication date: 2006-04-12
 
J. Anim. Feed Sci. 2006;15(2):305–311
 
KEYWORDS
ABSTRACT
Ten bales of grass silage were cored at eight pre-determined positions and the mould and yeast propagules were enumerated. Both mould and yeast numbers varied greatly between and within individual bales. Yeast colony forming units (cfu) showed greater variability between (s.e. 1.44 log10 cfu/g) than within bales (s.e. 0.53 log10 cfu/g). This variation between and within bales was statistically significant (P<0.05). When estimating the numbers of fungal propagules in bales that contain no visible mould or yeast growth, it is recommended that eight, or more, samples be taken per bale and these then composited to provide one representative sample of silage.
 
CITATIONS (5):
1.
Quantification and identification of fungal propagules in well-managed baled grass silage and in normal on-farm produced bales
Martin O’Brien, Padraig O’Kiely, Patrick D. Forristal, Hubert T. Fuller
Animal Feed Science and Technology
 
2.
Variation associated with sampling bale or pit silage for mycotoxins and conventional chemical characteristics
C. McElhinney, M. Danaher, J. Grant, C.T Elliott, P. O’Kiely
World Mycotoxin Journal
 
3.
Filamentous fungi in wrapped forages determined with different sampling and culturing methods
Jessica Schenck, Annika Djurle, Dan Funck Jensen, Cecilia Müller, Martin O'Brien, Rolf Spörndly
Grass and Forage Science
 
4.
Variations in bacterial communities in laboratory-scale and big bale silos assessed by fermentation products, colony counts and denaturing gradient gel electrophoresis profiles
N. Naoki, T. Yuji
Letters in Applied Microbiology
 
5.
Application of monoclonal antibodies in quantifying fungal growth dynamics during aerobic spoilage of silage
Cocq Le, Bethany Brown, Christopher Hodgson, Jamie McFadzean, Claire Horrocks, Michael Lee, David Davies
Microbial Biotechnology
 
ISSN:1230-1388