ORIGINAL PAPER
In vivo study on the persistence of transformed β-glucanase-producing Lactobacillus strains in the gastrointestinal tract of chickens
C. C. Sieo 1, 2  
,   N. Abdullah 3, 2,   W. S. Tan 1, 2,   Y. W. Ho 3
 
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1
University Putra Malaysia, Department of Microbiology, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia
2
Institute of Bioscience, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia
3
University Putra Malaysia, Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia
CORRESPONDING AUTHOR
C. C. Sieo   

University Putra Malaysia, Department of Microbiology, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia; Institute of Bioscience, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia
Publication date: 2006-04-12
 
J. Anim. Feed Sci. 2006;15(2):261–274
 
KEYWORDS
ABSTRACT
The persistence and ability of transformed Lactobacillus strains to function and produce β-glucanase in the gastrointestinal tract of chickens was investigated. Chickens were fed diets supplemented with 109 cells/kg of transformed Lactobacillus strains which harboured plasmid pSA3 carrying a gene encoding β-glucanase. The transformed β-glucanase-producing Lactobacillus strains were L. brevis C10CpSA3b6, L.crispatus I12pSA3b6, L. brevis I23pSA3b6, L. fermentum I25pSA3b6, L. brevis I211pSA3b6 and L. brevis I218pSA3b6. At days 14 to 18, digesta samples from the gizzard, duodenum, jejunum, ileum and caecum were collected daily and the presence of the respective β-glucanase-producing Lactobacillus strains was determined by a PCR-based method. Results of the study showed that the β-glucanase-producing Lactobacillus strains could persist in the gastrointestinal tract of the chickens for about two days. The strains were found to be alive and active in vivo, in which β-glucanase activity was significantly (P<0.05) higher in chickens fed diets supplemented with β-glucanase-producing Lactobacillus strains. Higher enzyme activities were located in the jejunum and ileum, especially in birds fed β-glucanase-producing L. brevis C10CpSA3b6 and L. brevis I211pSA3b6. The results indicated that the Lactobacillus strains could be a potential vehicle to deliver enzymes into the gastrointestinal tract of chickens.
 
CITATIONS (3):
1.
Coexpression and Secretion of Endoglucanase and Phytase Genes in Lactobacillus reuteri
Lei Wang, Yuxin Yang, Bei Cai, Pinghua Cao, Mingming Yang, Yulin Chen
International Journal of Molecular Sciences
 
2.
Transformation of, and Heterologous Protein Expression in,Lactobacillus agilisandLactobacillus vaginalisIsolates from the Chicken Gastrointestinal Tract
David P. Stephenson, Robert J. Moore, Gwen E. Allison
Applied and Environmental Microbiology
 
3.
Lactobacillus Strain Ecology and Persistence within Broiler Chickens Fed Different Diets: Identification of Persistent Strains
David Stephenson, Robert Moore, Gwen Allison
Applied and Environmental Microbiology
 
ISSN:1230-1388