Current issue
Online first
Archive
About the Journal
Editorial Office
Editorial Board
Copy right and self-archiving policy
Peer review process
Instructions for Reviewers
Printed version subscription
Abstracting and indexing
Contact
Instructions for Authors
Policies
General information
Open Access, Licensing terms, Commercial use and Copyright terms policies
Self-archiving policy and Archive policies
Article Correction and Withdrawal policy
Manuscript Submission policy
Authorship policy
Conflict of Interest policy
Language considerations policy
Plagiarism and Duplicate publications policy
Ethics policy
Review process policy
Acceptance of manuscripts policy
Online First Articles and Special Issues policies
Generative artificial intelligence (AI) policy
Advertising policy
Article publication charges
Policies
General information
Open Access, Licensing terms, Commercial use and Copyright terms policies
Self-archiving policy and Archive policies
Article Correction and Withdrawal policy
Manuscript Submission policy
Authorship policy
Conflict of Interest policy
Language considerations policy
Plagiarism and Duplicate publications policy
Ethics policy
Review process policy
Acceptance of manuscripts policy
Online First Articles and Special Issues policies
Generative artificial intelligence (AI) policy
Advertising policy
ORIGINAL PAPER
Dietary glucose oxidase supplementation improves growth performance, antioxidative capacity, and meat quality in finishing pigs
1
University of Nebraska-Lincoln, Department of Animal Science , Lincoln, NE 68583, USA
2
Dankook University, Department of Animal Resource & Science, Anseodong, Cheonan, Chungnam, 330-714, South Korea
These authors had equal contribution to this work
Publication date: 2026-07-03
Corresponding author
I. H. Kim
Dankook University, Department of Animal Resource & Science, Anseodong, Cheonan, Chungnam, 330-714, South Korea
Dankook University, Department of Animal Resource & Science, Anseodong, Cheonan, Chungnam, 330-714, South Korea
KEYWORDS
TOPICS
ABSTRACT
The purpose of this study was to assess the effects of dietary supplementation with glucose oxidase (GOX) on growth performance, faecal microbial populations, meat quality, faecal gas emissions, apparent nutrient digestibility, and serum antioxidant enzyme parameters in finishing pigs. A total of 200 finishing pigs [(Landrace × Yorkshire) × Duroc] were used in a 70-day trial. The pigs were randomly assigned to 4 groups based on their initial body weight (52.69 ± 0.58 kg) and sex. Each treatment consisted of 10 replicate pens with five pigs (gilts and barrows) per pen. The dietary treatments included a control diet and the control diet supplemented with 0.01, 0.02, or 0.03% GOX. The results showed linear increases in average daily gain (P = 0.038), average daily feed intake (P = 0.039), serum glutathione peroxidase activity (P = 0.009), and serum glutathione concentration (P = 0.005) with increasing dietary GOX supplementation. In contrast, drip loss on day 7 decreased linearly (P = 0.014) with the lowest value observed in pigs receiving 0.03% GOX. In conclusion, dietary supplementation with 0.03% GOX improved growth performance and meat quality likely by increasing antioxidant enzyme levels in a dose-dependent manner.
FUNDING
This research was supported by the Basic Science Research Programme through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF‐RS‐2023‐00275307).
CONFLICT OF INTEREST
The Authors declare that there is no conflict of interest.
REFERENCES (56)
1.
AOAC, 2000. Official method of analysis. 17th ed. Assoc. Off. Anal. Chem., Arlington, VA.
2.
Bankar S.B., Bule M.V., Singhal R.S., Ananthanarayan, L., 2009. Glucose oxidase - An overview. Biotechnol. Adv. 27, 489-501, https://doi.org/10.1016/j.biot....
3.
Bidner B.S., Ellis M., Brewer M.S., Campion D., Wilson E.R., McKeith F.K., 2004. Effect of ultimate pH on the quality characteristics of pork. J. Muscle Foods. 15, 139-154, https://doi.org/10.1111/j.1745....
4.
Castellini C., Mugnai C., Dal Bosco C., 2002. Effect of organic production system on broiler carcass and meat quality. Meat Sci. 60, 219-225, https://doi.org/10.1016/S0309-....
5.
Chen Q.H., Chen F.M., Xiao J., Li. H.B., 2015. Effects of glucose oxidase on growth performance, nutrient digestibility, and intestinal microflora and morphology of weaned piglets. Anim Nutr. 27, 3218-3224.
6.
Cruz A.G., Castro W.F., Faria J.A.F., Jr S.B., Granato D., Celeguini R.M.S., LimaPallone J., Godoy H.T., 2012. Glucose oxidase: A potential option to decrease the oxidative stress in stirred probiotic yogurt. LWT - Food Sci. Tech. 47, 512-515, https://doi.org/10.1016/j.lwt.....
7.
Dang D.X., Hoque M.R., Liu Y., Chen N., Kim I.H., 2021b. Dietary glucose oxidase supplementation improves growth performance, apparent nutrient digestibility, and serum antioxidant enzyme parameters in growing pigs. Ital J. Anim. Sci. 20, 1568-1574, https://doi.org/10.1080/182805....
8.
Dang D.X., Kim I.H., 2021. The effects of road transportation with or without homeopathic remedy supplementation on growth performance, apparent nutrient digestibility, fecal microbiota, and serum cortisol and superoxide dismutase levels in growing pigs. J. Anim. Sci. 2021, skab077, https://doi.org/10.1093/jas/sk....
9.
Dang D.X., Li Y.J., Kim I.H., 2020. Effects of dietary supplementation of enzymatic bioconversion of Scutellaria baicalensis extract as an alternative to antibiotics on the growth performance, nutrient digestibility, fecal microbiota, fecal gas emission, blood hematology, and antioxidative indicators in growing pigs. Livest Sci. 23, 104307, https://doi.org/10.1016/j.livs....
10.
Dang D.X., Liu Y., Chen N., Kim, I.H., 2021a. Dietary supplementation of Aspergillus niger-expressed glucose oxidase ameliorates weaning stress and improves growth performance in weaning pigs. J. Anim. Physiol. Anim. Nutr. 106, 258-265, https://doi.org/10.1111/jpn.13....
11.
Ferket P.R., van Heugten E., van Kempen T.A.T.G., Angel R., 2002. Nutritional strategies to reduce environmental emissions from nonruminants. J. Anim. Sci. 80, E168-E182, https://doi.org/10.2527/animal....
12.
Gaines A.M., Mendoza O.F., Estrada J.E., 2018. Practical formulation using corn coproducts and other alternative ingredients – new research, industry trends, and research gaps. J. Anim. Sci. 96, 64-65, https://doi.org/10.1093/jas/sk....
13.
Hu Q., Li S., Zhang Y., Zhuo Z., Feng J., 2017. Phytosterols on growth performance, antioxidant enzymes and intestinal morphology in weaned piglets. J. Sci. Food Agric. 97, 4629-4634, https://doi.org/10.1002/jsfa.8....
14.
Hou Z., Jiang, G., Wu D., Li C., Liu Y., Wang H., Dai Q., 2017. Effects of glucose oxidase on growth performance, serum biochemical indexes and antioxidant function, and nutrient digestibility of weaned piglets. Anim. Nutr. 29, 3482-3488.
15.
Liu X., Yin J., Kim I.H., 2019. Effect of protease derived from Pseudoalteromonas arctica supplementation on growth performance, nutrient digestibility, meat quality, noxious gas emission and blood profiles in finishing pigs. J. Anim. Physiol. Anim. Nutr. 103, 1926-1933, https://doi.org/10.1111/jpn.13....
16.
Liu X., Lee S.I., Kim I.H., 2020. Achyranthes japonica extracts supplementation to growing pigs positively influences growth performance, nutrient digestibility, fecal microbial shedding, and fecal gas emission. Asian-Australas. J. Anim. Sci. 34, 427-433, https://doi.org/10.5713/ajas.2....
17.
Long S., Hu J., Mahfuz S., Ma H., Piao, X., 2021. Effects of dietary supplementation of compound enzymes on performance, nutrient digestibility, serum antioxidant status, immunoglobulins, intestinal morphology and microbiota community in weaned pigs. Arch Anim Nutr. 75, 31-47, https://doi.org/10.1080/174503....
18.
Ma X.Y., Jiang Z.Y., Lin Y.C., Zheng C.T., Zhou G.L., 2010. Dietary supplementation with carnosine improves antioxidant capacity and meat quality of finishing pigs. J. Anim. Physiol. Anim. Nutr. 94, e286-e295, https://doi.org/10.1111/j.1439....
19.
Mehrabi Z., Gill M.L., van Wijk M., Herrero M., Ramankutty N., 2020. Livestock policy for sustainable development. Nat. Food. 1, 160-165, https://doi.org/10.1038/s43016....
20.
Munezero O., Kim I.H., 2022. Effects of protease enzyme supplementation in weanling pigs’ diet with different crude protein levels on growth performance and nutrient digestibility. J. Anim. Sci. Tech. 64, 854-862, https://doi.org/10.5187/jast.2....
21.
Munezero O., Muhizi S., Kim I.H., 2022b. Influence of protected organic acids on growth performance, fecal microbial composition, gas emission, and apparent total tract digestibility in growing pigs. Can. J. Anim. Sci. 102, 554–560, https://doi.org/10.1139/cjas-2....
22.
Nahm K.H., 2002. Efficient feed nutrient utilization to reduce pollutants in poultry and swine manure. Crit. Rev. Env. Sci. Tec. 32, 1-16, https://doi.org/10.1080/106433....
23.
Ngapo T.M., Martin J.F., Dransfield E., 2007. International preferences for pork appearance: I. Consumer choices. Food Qual. Prefer. 18, 26-36, https://doi.org/10.1016/j.food....
24.
NPPC, 1991. Procedures to evaluate market hogs (3rd Ed.), National Pork Producers Council: Des Moines, IA, USA.
25.
NPPC, 2000. Composition and quality assessment procedures, National Pork Producers Council: Des Moines, IA, USA.
26.
NRC, 2012. Nutrient requirements of swine. 11th rev. ed. National Academies Press, Washington, DC.
27.
Oliver W.T., Wells J.E., 2015. Lysozyme as an alternative to growth promoting antibiotics in swine production. J. Anim. Sci. Biotechnol. 6, 35, https://doi.org/10.1186/s40104....
28.
Park S., Lee J.J., Yang B.M. et al., 2020. Dietary protease improves growth performance, nutrient digestibility, and intestinal morphology of weaned pigs. J. Anim. Sci. Technol. 62, 21, https://doi.org/10.5187/jast.2....
29.
Pluske J.R., Turpin D.L., Kim J.C., 2018. Gastrointestinal tract (gut) health in the young pig. Anim. Nutr. 4, 187-196, https://doi.org/10.1016/j.anin....
30.
Qu W., Liu J., 2021. Effects of glucose oxidase supplementation on the growth performance, antioxidative and inflammatory status, gut function, and microbiota composition of broilers fed moldy corn. Front. Physiol. 12, 646393, https://doi.org/10.3389/fphys.....
31.
Rosenvold K., Andersen H.J., 2003. Factors of significance for pork quality—a review. Meat Sci. 64, 219-237, https://doi.org/10.1016/S0309-....
32.
Silva-Guillen Y.V., Arellano C., Boyd R.D., Martinez G., Van Heugten E., 2020. Growth performance, oxidative stress and immune status of newly weaned pigs fed peroxidized lipids with or without supplemental vitamin E or polyphenols. J. Anim. Sci. Biotechnol. 11, 22, https://doi.org/10.1186/s40104....
33.
Shigeno Y., Zhang H., Banno T., Usuda K., Nochi T., Inoue, R., Watanabe G., Jin W., Benno Y., Nagaoka K., 2019. Gut microbiota development in mice is affected by hydrogen peroxide produced from amino acid metabolism during lactation. FASEB J. 33, 3343-3352, https://doi.org/10.1096/fj.201....
34.
Tang H., Gao X., Yao B., 2013. Study on feed effects of glucose oxidase applied in piglets. China Feed. 2013, 19.
35.
Tang H., Yao B., Gao X., Yang P., Wang Z., Zhang G., 2016. Effects of glucose oxidase on the growth performance, serum parameters and faecal microflora of piglets. S. Afr. J. Anim. Sci. 46, 14-20, https://doi.org/10.4314/sajas.....
36.
Tang H., Gao X., Yao, B., Zhang G., Wang Z., Li X., 2015. Effects of glucose oxidase on growth performance, serum parameters and slaughter performance of meat ducks and its antidotal effect on aflatoxin B. Anim. Nutr. 27, 2361-2367.
37.
Tian D., Zhang Y., Tian Q., 2012. Control of piglet early weaning diarrhea by adding glucose oxidase and phytohemagglutinin to diet. Anim. Husbandry Feed Sci. 2012, 10.
38.
Traore S., Aubry L., Gatellier P., Przybylski W., Jaworska D., Kajak-Siemaszko K., Santé-Lhoutellier V., 2012. Higher drip loss is associated with protein oxidation. Meat Sci. 90, 917-924, https://doi.org/10.1016/j.meat....
39.
Wang F., Chen X., Wang Y., Li X., Wan M., Zhang G., Leng F., Zhang H., 2022a. Insights into the structures, inhibitors, and improvement strategies of glucose oxidase. Int. J. Mol. Sci. 23, 9841, https://doi.org/10.3390/ijms23....
40.
Wang H., Kim K.P., Kim I.H., 2019. Influence of Bacillus subtilis GCB‐13‐001 on growth performance, nutrient digestibility, blood characteristics, faecal microbiota and faecal score in weanling pigs. J. Anim. Physiol. Anim. Nutr. 103, 1919-1925, https://doi.org/10.1111/jpn.13....
41.
Wang Y., Wang Y., Xu H., Mei X., Gong L., Wang B., Li W., Jiang S., 2018. Direct-fed glucose oxidase and its combination with B. amyloliquefaciens SC06 on growth performance, meat quality, intestinal barrier, antioxidative status, and immunity of yellow-feathered broilers. Poult. Sci. 97, 3510-3549, https://doi.org/10.3382/ps/pey....
42.
Wang W., Xie R., Cao Q., Ye H., Zhang C., Dong Z., Feng D., Zuo J., 2022b. Effects of glucose oxidase on growth performance, clinical symptoms, serum parameters, and intestinal health in piglets challenged by enterotoxigenic Escherichia coli. Front. Microbiol. 13, https://doi.org/10.3389/fmicb.....
43.
Wu D., Cheng C., Dai Q., 2015. Appropriate addition of glucose oxidase and effectiveness comparison with zinc oxide in diet for weaned piglets. Acta Ecol. Anim. Domast. 36, 31-35.
44.
Wu S., Chen X., Li T., Ren H., Zheng L., Yang X., 2020. Changes in the gut microbiota mediate the differential regulatory effects of two glucose oxidases produced by Aspergillus niger and Penicillium amagasakiense on the meat quality and growth performance of broilers. J. Anim. Sci. Biotechnol. 11, 73, https://doi.org/10.1186/s40104....
45.
Wu S., Li T., Niu H., Zhu Y., Liu Y., Duan Y., Sun Q., Yang X., 2019. Effects of glucose oxidase on growth performance, gut function, and cecal microbiota of broiler chickens. Poult. Sci. 98, 828-841, https://doi.org/10.3382/ps/pey....
46.
Yu J., Ahmedna M., Goktepe I., 2010. Potential of peanut skin phenolic extract as antioxidative and antibacterial agent in cooked and raw ground beef. Int. J. Food Sci. Tech. 45, 1337-1344, https://doi.org/10.1111/j.1365....
47.
Yu J., Qian C., Zhang Y., Cui Z., Zhu Y., Shen Q., Ligler F.S., Buse J.B., Gu Z., 2017. Hypoxia and H2O2 Dual-Sensitive Vesicles for Enhanced Glucose-Responsive Insulin Delivery. Nano Lett. 17, 733-739, https://doi.org/10.1021/acs.na....
48.
Yuan S., Chen D., Zhang K., Yu B., 2007. Effects of oxidative stress on growth performance, nutrient digestibilities and activities of antioxidative enzymes of weanling pigs. Asian-Austral. J. Anim. Sci. 20, 1600-1605, https://doi.org/10.5713/ajas.2....
49.
Zhao Y., Fu J., Li P., Chen N., Liu Y., Liu D., Guo Y., 2022. Effects of dietary glucose oxidase on growth performance and intestinal health of AA broilers challenged by Clostridium perfringens. Poultry Sci. 101, 101553, https://doi.org/10.1016/j.psj.....
50.
Zhang A.W., Ju G.C., Xue J., Zuo L.Y., Dong B., 2011. Effects of yeast (Saccharomyces cerevisiae) and yeast extracts on meat quality of broiler chickens (in Chinese). Anim. Nutr. 23, 299-306, https://doi.org/10.3969/j.issn....
51.
Zhang J., Liu Y., Yang Z., Yang W., Huang L., Xu C., Liu M., Ge J., Wang Y., Jiang S., 2020. Illicium verum extracts and probiotics with added glucose oxidase promote antioxidant capacity through upregulating hepatic and jejunal Nrf2/Keap1 of weaned piglets. J. Anim. Sci. 98, skaa077, https://doi.org/10.1093/jas/sk....
52.
Zhang G.G., Yang Z.B., Wang Y., Yang W.R., Zhou H.J., 2014. Effects of dietary supplementation of multi-enzyme on growth perfor-mance, nutrient digestibility, small intestinal digestive enzyme activities, and large intestinal selected microbiota in weanling pigs. J. Anim. Sci. 92, 2063-2069, https://doi.org/10.2527/jas.20....
53.
Zhao Y., Cui Y., Chen B., Sun H., Li S., Li T., 2014. Glucose oxidase: effects on body weight and intestinal flora of mice with moldy feed. Anim. Nutr. 26, 3531-3536.
54.
Zhao W., Huang Y., Cui N., Wang R., Xiao Z., Su X., 2023. Glucose oxidase as an alternative to antibiotic growth promoters improves the immunity function, antioxidative status, and cecal microbiota environment in white-feathered broilers. Front. Microbiol. 14, https://doi.org/10.3389/fmicb.....
55.
Zou L., Xiong X., Liu H., Zhou J., Liu Y., Yin Y., 2019. Effects of dietary lysozyme levels on growth performance, intestinal morphology, immunity response and microbiota community of growing pigs. J. Sci. Food. Agric. 99, 1643-1650, https://doi.org/10.1002/jsfa.9....
56.
Zou Y., Xiang Q., Wang J., Wei H., Peng J., 2016. Effects of oregano essential oil or quercetin supplementation on body weight loss, carcass characteristics, meat quality and antioxidant status in finishing pigs under transport stress. Livest. Sci. 192, 33-38, https://doi.org/10.1016/j.livs....
Share
RELATED ARTICLE
| ISSN: | 1230-1388 |
Assigning DOI numbers, introducing articles from supplements and recent publications to POL-index database, maintaining anti-plagiarism detection, electronic system to proceed manuscripts and webpage of the Journal with interactive pdf files, and language correction of manuscripts published in Journal of Animal and Feed Sciences are financed in the years 2018–2019 by the Ministry of Science and Higher Education from the funds for science popularization activities, Agreement No. 631/P-DUN/2018.
© 2006-2026 Journal hosting platform by Bentus
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
