The influence of cellulose, starch and different sources of nitrogen on the growth of rumen ciliate protozoon Epidinium ecaudatum in vitro
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The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
Publication date: 1995-08-22
J. Anim. Feed Sci. 1995;4(3):255–261
The rumen ciliate Epidinium ecaudatum was grown in vitro in one species culture. The culture medium consisting of salt solution, powdered hay and wheat gluten provided appropriate conditions for maintenance of the protozoa. The population density was influenced by the proportion of cellulose, starch and urea in the culture medium as well as by the solubility of protein. The ciliates did not require rumen fluid for the growth.
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